Analysis of microbiota in the stomach and midgut of two penaeid shrimps during probiotic feeding

Imaizumi, Kentaro; Tinwongger, Sasiwipa; Kondo, Hidehiro; Hirono, Ikuo

doi:10.1038/s41598-021-89415-w

Cited by 31 publications

(16 citation statements)

References 55 publications

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“…Additionally, fish fed D3, D4 ,and D5 showed better digestibility of crude protein and crude lipid than the control D1. An investigation of the retention of probiotic bacteria BA in the digestive tract of penaeid shrimp (L. vannamei and Marsupenaeus japonicas) revealed their presence in all sections of the digestive tract, with the stomach content having a significantly high count [16], suggesting better digestive activity when combine with other strain (D2) as reported in the present study.…”

supporting

confidence: 82%

“…The supplementation of Bacilli probiotic (Bacillus subtilis and Bacillus licheniformis) in white leg shrimp, Litopenaeus vannamei, improved growth performance parameters and increased total protein, lysozyme, and hemocyte cell count [9]. The dietary inclusion of Bacillus amyloliquefaciens (BA) significantly improves condition factor and relative condition factor in amberjack, Seriola dumerili [10]; improves specific growth rate (SGR) and feed conversion ratio (FCR) in Nile tilapia, Oreochromis niloticus [11,12]; improves disease resistance against Aeromonas hydrophila infection in eels [13]; improves immune responses in catla fish (Catla catla) and channel catfish, Ictalurus punctatus [14,15]; and improves the survival rate of L. vannamei challenged with Vibrio parahaemolyticus [16]. The individual strain of probiotic bacteria such as Lactobacillus plantarum (LP) was reported to improve feed intake (FI), SGR, weight percent, oxidative status, and immune enzyme in koi carp (Carassius auratus) [17].…”

Section: Introductionmentioning

confidence: 99%

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Effects of Single and Mixture Probiotic Supplements on Growth, Digestive Activity, Antioxidative Status, Immune and Growth-Related Genes, and Stress Response of Juvenile Red Sea Bream (Pagrus Major)

Shadrack

Ishikawa

Koshio

et al. 2022

Aquaculture Nutrition

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A 50-day feeding trial was conducted to evaluate the efficiency of single and mixed strains of probiotic bacteria supplements on juvenile red sea bream (Pagrus major). The study investigated the growth, digestibility, hemato-biochemistry, antioxidant, immune, immune, and growth gene expression and stress responses of P. major. Three hundred juvenile P. major ( 21.56 ± 0.09 g) were randomly distributed into fifteen 200-L polyethylene tanks (20 fish per tank) in triplicate of 5 treatments designated as D1 = the basal diet; Streptococcus faecalis (SF) T − 110 5 × 106 cfu/g diet and Bacillus amyloliquefaciens (BA) TOA 5001 5 × 105 cfu/g diet in the second group (D2) at 0.2%; mix SF T − 110 1 × 106 cfu/g diet, Lactobacillus plantarum (LP) TO − A 4 × 105 cfu/g diet, Bacillus mesentericus (BM) TO − A 1 × 102 cfu/g diet, BA TOA 5001 5 × 105 cfu/g diet in the third group (D3) at 1%; mix SF T − 110 1 × 106 cfu/g diet, LP TO − A 4 × 105 cfu/g diet, and BM TO − A 1 × 102 cfu/g diet in fourth group (D4) at 0.5%; and single strain BA TOA5001 5×105 cfu/g diet in the fifth group (D5) at 0.5% of dietary proportion. Results showed that D2 and D3 fish groups exhibit better performance, followed by D4, D5, and control group D1. This finding demonstrated that the use of BA in mix strain probiotic bacteria diet (D2, D3) had improved immune response, antioxidant enzymes, immunity (TNF-a, IL-1b), and growth-related (IGF-1, IGF-2) mRNA expression of juvenile P. major compared to the mix strain D4, single strain D5, and the control D1. Furthermore, single strain D5 and mix strain D4 also exhibit relatively better immune responses in P. major than in control D1. Considering the overall fish performances, mix strain SF+BA (D2) and mix strain SF+BM+LP+BA (D3) were the recommended potential mix strain probiotic bacteria supplement for P. major and may be useful also for other related aquatic species.

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supporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Effects of Single and Mixture Probiotic Supplements on Growth, Digestive Activity, Antioxidative Status, Immune and Growth-Related Genes, and Stress Response of Juvenile Red Sea Bream (Pagrus Major)

Shadrack

Ishikawa

Koshio

et al. 2022

Aquaculture Nutrition

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“…The intestinal microbiome has been studied in a variety of aquatic animals and its importance in health is becoming more and more obvious (Danckert et al, 2021; Imaizumi et al, 2021; King et al, 2019; Steiner et al, 2021). However, to our knowledge, the study of microbial diversity of B. areolata has not been reported.…”

Section: Discussionmentioning

confidence: 99%

“…Currently, studies on intestinal microbiome of fish and shrimp were widely carried out and its importance in health is becoming more and more obvious (Imaizumi et al, 2021; Steiner et al, 2021). In molluscs, such as oyster and abalone, the roles of microbiome also were explored.…”

Section: Introductionmentioning

confidence: 99%

Gastrointestinal tract microbial community of Babylonia areolata and its diversity are closely correlated with the outbreak of disease

Zhao

Wang

et al. 2021

Aquaculture Research

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The intestinal microbiome is important for the health of aquatic animals. However, in Babylonia, the combination of gastrointestinal tract (GI) bacterial symbionts has barely been explored. In this study, from a farm with naturally occurring diseases, we collected Babylonia areolata in three different states, that is “diseased” (group D), “health” (group H) and “subhealth” (group SH) and aimed to reveal the relationship between disease occurrence and GI microbiota of B. areolata. Diversity analysis illustrated that GI microbial diversity of group D was obviously lower than that of group H on phylum level and genus level, and GI microbial diversity of group SH was between group D and H. KEGG analysis showed that the physiological activities of pathogenic microorganisms in group D increased significantly, compared with group H. Metagenomics analysis demonstrated the expressions of virulence genes in group D enhanced obviously, compared with group H. In addition, the diversity of culturable microorganisms from the GI contents of group D was dramatically lower than that of group H. A total of 22 species were obtained from the GI contents of group H. However, only four species were obtained from group D, and two important pathogens, Vibrio harveyi and Vibrio tubiashii, were dominant microbiota. These results indicate that the decline of GI microbial diversity in B. areolata is highly associated with the occurrence of disease. We speculated environmental factors led to the decline of GI microbial diversity, which could promote propagation of pathogenic microbes (such as V. harveyi and V. tubiashii). Our findings provide new insights into the disease outbreak of B. areolata and provide propose that the preservation of GI microbial diversity at the early phases can effectively prevent the occurrence of disease.

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“…Therefore, since the type of food taken by the shrimp is an important factor in determining gut microbial composition (Pedrosa‐Gerasmio et al, 2020), it is important to consider the impact of the compounds added to the diet on the microbial profile of the intestinal tract of the shrimp (Imaizumi et al, 2021), such as when supplementing QS inhibitors in feeds.…”

Section: Potential Limitations On the Use Of Quorum Sensing Inhibitorsmentioning

confidence: 99%

Targeting quorum sensing and biofilm formation in the control of Vibrio harveyi infections in Penaeus vannamei

Guzman

Yatip

Soowannayan

et al. 2022

Aquaculture Research

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Vibriosis, a disease caused by Vibrio spp., including V. harveyi, contributes to enormous economic loss to the shrimp aquaculture industry. With the worsening incidences of antimicrobial resistance in aquaculture, novel strategies for combating this bacterial infection are urgently needed. One of these is by targeting the bacterial communication system, called quorum sensing (QS), which is responsible for the expression of genes necessary for bacterial pathogenesis. Recently, bioactive compounds capable of inhibiting QS were identified. This review focuses on the discovery of bioactive substances from animal, plant, and microbial origins with the capability of inhibiting QS and biofilm formation, a mechanism important in the pathogenesis of vibriosis. Furthermore, recent developments in the use of these QS inhibitors (QSIs) as feed supplement for Penaeus vannamei shrimp were also discussed. These findings will provide the necessary information for the discovery, development, and eventual commercialization of these QSIs as tools in mitigating vibriosis in the shrimp industry.

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Analysis of microbiota in the stomach and midgut of two penaeid shrimps during probiotic feeding

Cited by 31 publications

References 55 publications

Effects of Single and Mixture Probiotic Supplements on Growth, Digestive Activity, Antioxidative Status, Immune and Growth-Related Genes, and Stress Response of Juvenile Red Sea Bream (Pagrus Major)

Effects of Single and Mixture Probiotic Supplements on Growth, Digestive Activity, Antioxidative Status, Immune and Growth-Related Genes, and Stress Response of Juvenile Red Sea Bream (Pagrus Major)

Gastrointestinal tract microbial community of Babylonia areolata and its diversity are closely correlated with the outbreak of disease

Targeting quorum sensing and biofilm formation in the control of Vibrio harveyi infections in Penaeus vannamei

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