Analysis of microRNAs expressions in chondrosarcoma

Abstract

MicroRNAs (miRNAs) are small noncoding RNAs capable of inhibiting gene expression post-transcriptionally and expression profiling can provide therapeutic targets and tools for cancer diagnosis. Chondrosarcoma is a mesenchymal tumor with unknown cause and differentiation status. Here, we profiled miRNA expression of chondrosarcoma, namely clinical samples from human conventional chondrosarcoma tissue, established chondrosarcoma cell lines, and primary non-tumorous adult articular chondrocytes, by miRNA array and quantitative real-time PCR. A wide variety of miRNAs were differently downregulated in chondrosarcoma compared to non-tumorous articular chondrocytes; 27 miRNAs: miR-10b, 23b, 24-1*, 27b, 100, 134, 136, 136*, 138, 181d, 186, 193b, 221*, 222, 335, 337-5p, 376a, 376a*, 376b, 376c, 377, 454, 495, 497, 505, 574-3p, and 660, were significantly downregulated in chondrosarcoma and only 2: miR-96 and 183, were upregulated. We further validated the expression levels of miRNAs by quantitative real-time PCR for miR-181a, let-7a, 100, 222, 136, 376a, and 335 in extended number of chondrosarcoma clinical samples. Among them, all except miR-181a were found to be significantly downregulated in chondrosarcoma derived samples. The findings provide potential diagnostic value and new molecular understanding of chondrosarcoma.

CORR Insights®: MicroRNA Regulates Vascular Endothelial Growth Factor Expression in Chondrosarcoma Cells

“…Normal chondrocytes, chondrosarcoma, and osteosarcoma have been shown to express miRNA-181a [1,2,6,9]. The current study has made an important contribution in terms of how it demonstrates a pathogenic role for miR181a, particularly in terms of the upregulation of VEGF, and how it may respond to hypoxia in chondrosarcoma cells.…”

“…Researchers must confirm the roles of miRNA-181a in a larger series of tumors, as well as check the correlation between vascularity with VEGF and miRNA-181a in the tissue specimen. The effects of hypoxia and miRNAs seemed more synergistic in an earlier report [9]. If this was indeed the case, then did the effects of hypoxia and miRNA-181a for VEGF overexpression work through different mechanisms or pathways?…”

“…Previous studies [1,2,6,9] demonstrated that miRNA-181a is neither cell nor tumor specific. Normal chondrocytes, chondrosarcoma, and osteosarcoma have been shown to express miRNA-181a [1,2,6,9].…”

“…A wide variety of miRNAs were downregulated differently in chondrosar -coma compared to nontumorous articular chondrocytes. The results from the study by Yoshitaka and colleagues [9] showed 27 downregulated miRNAs with only miR-96 and 183 upregulated.…”

“…Previous research [5] demonstrated the roles of miRNAs in tumor initiation, invasion, angiogenesis, apoptosis, and metastasis, among other areas. A number of articles [1][2][3][4][5][6][7][8][9] have investigated miRNAs in sarcomas. Yoshitaka and colleagues [9] evaluated the miRNA expressions in clinical samples from human conventional chondrosarcoma tissue, cell lines, and nontumorous articular chondrocytes.…”