Analysis of microsamples by miniaturized magnetic-based pipette tip microextraction: determination of free cortisol in serum and urine from very low birth weight preterm newborns

Grau, José; Moreno‐Guzmán, María; Arruza, Luis; López, Miguel Ángel; Escarpa, Alberto; Chisvert, Alberto

doi:10.1039/d2an02085h

Cited by 8 publications

(3 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Extraction device of miniaturized magnetic‐based pipette tip microextraction. Adapted with permission [69]. …”

Section: Miniaturization Of Sorbent‐based Approachesmentioning

confidence: 99%

“…Thus, PT-μSPE has also adopted the dispersive modality either by aspiratingdispensing cycles in disposable-pipette extraction (DPX) [66] or by effervescence [67]. Moreover, more recently, Grau et al incorporated the benefits of dispersion of magnetic nanomaterials into the pipette tip format and applied them to the analysis of 400 μl of saliva [68] or 10 μl of serum in its most miniaturized version [69] (Figure 4). Briefly, it involves quick dispersion of a magnetic sorbent material in the sample to entrap the target analytes, and its subsequent aspiration using a (semi)automatic pipette through a pipette tip with a small cubic neodymium magnet inside, which retrieves the magnetic sorbent containing the analytes.…”

Section: Micro-solid Phase Extractionmentioning

confidence: 99%

See 1 more Smart Citation

Ultramicroextraction as a miniaturization of the already miniaturized. A step toward nanoextraction and beyond

Azorín

Benedé

Chisvert

2023

J of Separation Science

Self Cite

View full text Add to dashboard Cite

Miniaturization of the analytical process has been a widespread trend, and the sample preparation stage is not exempted from this downscaling. Since the introduction of microextraction techniques as miniaturization of classical extraction techniques, they have become one of the strengths in this field. However, some of the original approaches to these techniques did not fully cover all the current principles of Green Analytical Chemistry. For this reason, during the last years, much emphasis has been placed on reducing/eliminating toxic reagents, reducing the amount of the extraction phase, and searching for new greener, and more selective extractant materials. On the other hand, even though high accomplishments have been achieved, the same attention has not always been paid to reducing the amount of sample, which is essential when treating low‐availability samples such as biological samples, or in developing portable devices. In this review, we intend to give the readership an overview of the advances toward further miniaturization of microextraction techniques. Finally, a brief reflection is made on the terminology used, or that should, in our opinion, be used to term these new generation of miniaturized microextraction approaches. To this regard, the term, ‘ultramicroextraction’ is proposed to refer to those approaches beyond microextraction.

show abstract

“…Extraction device of miniaturized magnetic‐based pipette tip microextraction. Adapted with permission [69]. …”

Section: Miniaturization Of Sorbent‐based Approachesmentioning

confidence: 99%

Section: Micro-solid Phase Extractionmentioning

confidence: 99%

Ultramicroextraction as a miniaturization of the already miniaturized. A step toward nanoextraction and beyond

Azorín

Benedé

Chisvert

2023

J of Separation Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…Magnetic-based systems have been proposed as promising alternatives for highly sensitive biological analyte detection, as they are not influenced by the magnetic background of biological samples [7]. Magnetic biosensors detect changes in the biological sensing element through alterations in magnetic field or susceptibility.…”

Section: Introductionmentioning

confidence: 99%

Modeling and Simulation of a High-Performance Magnetic Biosensor for Biomedical Sensing

2024

EOIJ

View full text Add to dashboard Cite

The current magnetic-based biosensor technologies are expensive and intricate, making them unsuitable for meeting the requirements of point-of-care medical diagnosis. This research introduces a straightforward magnetic biosensor architecture that includes an L-shaped ferromagnetic core with UL dimensions. The design involves an air gap being replaced with highly porous aluminum or copper foam, offering a potentially cost-effective and uncomplicated solution for point-of-care diagnosis based on the magnetic field effect. The foam serves as a medium for hosting biological samples, such as proteins and DNA, which are labeled with high-permeability ferromagnetic nanoparticles. The biosensor operates by detecting labeled biological molecules through magnetic field interactions. The electrical parameters of the system underwent methodical optimization to enhance overall performance. The investigation delved into the influence of various materials on the magnetic properties of the air gap. It also examined the relationships between permeability, output-induced voltage, input voltage, and input frequency. The findings reveal that utilizing materials with elevated magnetic permeability, such as Magnetite (Fe3 O4 ) or Cobalt ferrite (CoFe2 O4 ) ferrofluids, significantly enhances the biosensor's performance by optimizing magnetic coupling between primary and secondary windings. This innovative magnetic biosensor exhibits potential applications in diverse fields, including molecular biology and medical diagnostics. The study contributes valuable insights into the design and optimization of magnetic biosensors, offering opportunities for heightened sensitivity and selectivity in the detection of ferromagnetic nanoparticles labeled biomolecules such as DNA or proteins.

show abstract

Single-drop microextraction

Gupta,

Jain,

Verma

2025

Green Analytical Methods and Miniaturized Sample Preparation Techniques for Forensic Drug Analysis

View full text Add to dashboard Cite

Analysis of microsamples by miniaturized magnetic-based pipette tip microextraction: determination of free cortisol in serum and urine from very low birth weight preterm newborns

Abstract: Miniaturized magnetic-based pipette tip microextraction is presented as a sample preparation approach for microsamples. It consists of the quick dispersion of a diminutive amount of a magnetic sorbent material in...

Cited by 8 publications

References 32 publications

Ultramicroextraction as a miniaturization of the already miniaturized. A step toward nanoextraction and beyond

Ultramicroextraction as a miniaturization of the already miniaturized. A step toward nanoextraction and beyond

Modeling and Simulation of a High-Performance Magnetic Biosensor for Biomedical Sensing

Single-drop microextraction

Contact Info

Product

Resources

About