Analysis of Microsatellite DNA Markers Reveals no Genetic Differentiation between Wild and Hatchery Populations of Pacific Threadfin in Hawaii

Pan, Gang; Yang, Jinzeng

doi:10.7150/ijbs.6.827

Cited by 19 publications

(10 citation statements)

References 26 publications

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“…Briefly, tissue samples were digested with 0.5 g/l proteinase K at 55°C overnight. The resulting solution was centrifuged; the supernatant was extracted by phenol-chloroform and precipitated in ethanol and dissolved in 1X TE buffer [15]. The quality and concentration of DNA were assessed by spectrophotometer and agarose gel electrophoresis, and DNA samples were stored at 4°C until use.…”

Section: Methodsmentioning

confidence: 99%

Identifications of Captive and Wild Tilapia Species Existing in Hawaii by Mitochondrial DNA Control Region Sequence

Yang

2012

PLoS ONE

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BackgroundThe tilapia family of the Cichlidae includes many fish species, which live in freshwater and saltwater environments. Several species, such as O. niloticus, O. aureus, and O. mossambicus, are excellent for aquaculture because these fish are easily reproduced and readily adapt to diverse environments. Historically, tilapia species, including O. mossambicus, S. melanotheron, and O. aureus, were introduced to Hawaii many decades ago, and the state of Hawaii uses the import permit policy to prevent O. niloticus from coming into the islands. However, hybrids produced from O. niloticus may already be present in the freshwater and marine environments of the islands. The purpose of this study was to identify tilapia species that exist in Hawaii using mitochondrial DNA analysis.Methodology/Principal FindingsIn this study, we analyzed 382 samples collected from 13 farm (captive) and wild tilapia populations in Oahu and the Hawaii Islands. Comparison of intraspecies variation between the mitochondrial DNA control region (mtDNA CR) and cytochrome c oxidase I (COI) gene from five populations indicated that mtDNA CR had higher nucleotide diversity than COI. A phylogenetic tree of all sampled tilapia was generated using mtDNA CR sequences. The neighbor-joining tree analysis identified seven distinctive tilapia species: O. aureus, O. mossambicus, O. niloticus, S. melanotheron, O. urolepies, T. redalli, and a hybrid of O. massambicus and O. niloticus. Of all the populations examined, 10 populations consisting of O. aureus, O. mossambicus, O. urolepis, and O. niloticus from the farmed sites were relatively pure, whereas three wild populations showed some degree of introgression and hybridization.Conclusions/SignificanceThis DNA-based tilapia species identification is the first report that confirmed tilapia species identities in the wild and captive populations in Hawaii. The DNA sequence comparisons of mtDNA CR appear to be a valid method for tilapia species identification. The suspected tilapia hybrids that consist of O. niloticus are present in captive and wild populations in Hawaii.

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Section: Methodsmentioning

confidence: 99%

Identifications of Captive and Wild Tilapia Species Existing in Hawaii by Mitochondrial DNA Control Region Sequence

Yang

2012

PLoS ONE

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“…Such adverse genetic effects on natural population restoration have been previously reported by Araki et al 2007;Cheng et al 2011 andLi et al 2016. Nevertheless, there have also been reports on maintaining genetic variability in hatchery populations of other fish species (Pan and Yang 2010;.…”

Section: Introductionmentioning

confidence: 68%

Genetic variability in wild and hatchery populations of commercially important fish: The common carp (Cyprinus carpio)

et al. 2018

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Ahmadi M, Kashiri H, Shabani A, Moghadam AA. 2018. Genetic variability in wild and hatchery populations of commerciallyimportant fish: The common carp (Cyprinus carpio). Biodiversitas 19: 1468-1474. Common carp, Cyprinus carpio, is considered as oneof the most important bony fish with high economic value in the Caspian sea. Since the population size of common carp has decreasedduring recent decades, restocking of the populations is done through releasing the hatchery-produced larvae into the Caspian Sea. In thepresent study, the genetic diversity of hatchery and wild populations of C. carpio was investigated using eight microsatellite loci(MFW7, MFW9, MFW13, MFW16, MFW17, MFW20, MFW26 and MFW28). A total of 145 different alleles were observed across allloci, with some of them being unique to each population. Although the allelic and gene diversity of hatchery populations tended to belower compared to the wild populations, no significant differences (P>0.05) in genetic diversity parameters were observed among thewild and hatchery populations. In most cases, significant departure from Hardy-Weinberg equilibrium was observed, mainly because ofthe heterozygosity deficiency. Results from FST, RST and UPGMA analysis showed that the hatchery-reared population was the mostdifferentiated and distant group. The results from this study are anticipated to provide important information for setting up moreefficient strategies in conservation and restocking of C. carpio.

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“…Similar to the present study, a low level of population differentiation (FST)was also observed among populations and significant differentiation (P<0.05) was evident only between the Halda riverine and hatchery populations in Bangladesh in Catla catla . Another recent study found that there is no genetic differentiation (FST = 0.001) between wild and hatchery population of Paciifc threadfin in Hawaii (Pan & Yang, 2010). Homogeneity tests for pair-wise comparisons of different stocks showed that the HatcheryHatchery♂ × Padma♀ populations are more homogeneous compared to the Padma-Hatchery♂ × Padma♀ and Padma-Hatchery population pairs (Table 5).…”

Section: Discussionmentioning

confidence: 99%

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Shah¹,

Sutapa²,

Islam³

et al. 2017

Turk. J. Fish. Aquat. Sci.

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Labeo rohita is an important commercial aquaculture species in Bangladesh, but it needs improved growth performance to meet the increasing demand for fish. Genetic improvement through inter-strain crossing, generating heterosis, is quicker than a selection strategy. Therefore, crosses were made between two strains of rohu: Padma River strain and a hatchery strain. Growth performance analyis showed the highest growth rate in the Padma × Padma cross followed by one of the reciprocal crosses Hatchery♂ × Padma♀ (hybrid). The lowest growth rate was observed in Hatchery × Hatchery cross. DNA variability study using three microsatellite markers showed polymorphism (P95) in these crosses. Variations were observed in number and frequencies of alleles and mean heterozygosity in different loci; the mean number of alleles varied from 4.667±0.882, 3.333±1.333 and 2.667±1.202 in Padma, hybrid and Hatchery strains respectively. Relatively high levels of gene flow and low Strain differentiation (FST ) values were found between the Padma and hybrid strains. The UPGMA dendrogram based on genetic distance placed the Padma population in one cluster and the hybrid and Hatchery populations in another. The results revealed that there was relatively low level of genetic variability in the riverine and hatchery reared populations of L. rohita in Bangladesh.

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Analysis of Microsatellite DNA Markers Reveals no Genetic Differentiation between Wild and Hatchery Populations of Pacific Threadfin in Hawaii

Cited by 19 publications

References 26 publications

Identifications of Captive and Wild Tilapia Species Existing in Hawaii by Mitochondrial DNA Control Region Sequence

Identifications of Captive and Wild Tilapia Species Existing in Hawaii by Mitochondrial DNA Control Region Sequence

Genetic variability in wild and hatchery populations of commercially important fish: The common carp (Cyprinus carpio)

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