Analysis of mixed lipid extracts using <sup>1</sup>H NMR spectra

Sparling, Mary Lee

doi:10.1093/bioinformatics/6.1.29

Cited by 6 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In order to determine experimentally the final composition of the nanoparticles with and without Miglyol, we dried the samples of isolated nanoparticles and dissolved them in d-chloroform for further 1 H NMR analysis. 1 H NMR has already been used for the quantification of lipid mixtures different from those presented here [22,23]. However, in the present study, a special intricacy was the quantification of the ratio of tripalmitin and Miglyol, due to the overlapping signals of these triglycerides which only differ in their aliphatic chain length.…”

Section: Analysis Of the Nanoparticle Compositionmentioning

confidence: 76%

Design and characterization of a new drug nanocarrier made from solid–liquid lipid mixtures

García-Fuentes

Alonso

Torres

2005

Journal of Colloid and Interface Science

View full text Add to dashboard Cite

Section: Analysis Of the Nanoparticle Compositionmentioning

confidence: 76%

Design and characterization of a new drug nanocarrier made from solid–liquid lipid mixtures

García-Fuentes

Alonso

Torres

2005

Journal of Colloid and Interface Science

View full text Add to dashboard Cite

“…Using a representative set of spectra, a pattern file defining bin boundaries was generated for all peak positions. Where possible peaks were identified using and in-house Avanti Library or annotated according to published identities [23,24]. The pattern file was validated using the TameNMR Galaxy toolkit (https://github.com/PGB-LIV/tameNMR) and then used to integrate the spectral peaks data into numerous 'bins' for statistical analysis.…”

Section: Spectra Inclusion Criteriamentioning

confidence: 99%

A robust method for performing 1H-NMR lipidomics in embryonic zebrafish

et al. 2021

Preprint

View full text Add to dashboard Cite

The embryonic zebrafish is an ideal system for lipid analyses with relevance to many areas of bioscience research, including biomarkers and therapeutics. Research in this area has been hampered by difficulties in extracting, identifying and quantifying lipids. We employed 1H-NMR at 700MHz to profile lipids in developing zebrafish embryos. The optimal method for lipidomics in embryonic zebrafish incorporated rapid lipid extraction using chloroform and an environment without oxygen depletion. Pools of 10 embryos gave the most acceptable signal-to-noise ratio, and the inclusion of chorions in the sample had no significant effect on lipid abundances. Embryos, bisected into cranial (head and yolk sac) and caudal (tail) regions, were compared by principal component analysis and analysis of variance. The lipid spectra (including lipid annotation) are available in the public repository MetaboLights (MTBLS2396)

show abstract

“…FFA accumulation could adversely affect the cell–cell contact and promotes tissue invasion and positively correlated with the extend of metastasis [ 10 ]. Inhibition of the lipogenesis either by pharmacological drugs or using antisense shRNA resulted in the inhibition of tumor growth and metastasis after anti-angiogenic treatment [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Proton NMR characterization of intact primary and metastatic melanoma cells in 2D & 3D cultures

Ramachandran

Yeow

2017

Biol Res

View full text Add to dashboard Cite

ObjectiveTo characterize the differences between the primary and metastatic melanoma cell lines grown in 2D cultures and 3D cultures.MethodsPrimary melanoma cells (WM115) and metastatic melanoma cells (WM266) extracted from a single donor was cultured in 2D as well as 3D cultures. These cells were characterized using proton NMR spectrometry, and the qualitative chemical shifts markers were identified and discussed.ResultsIn monolayer culture (2D), we observed one qualitative chemical shift marker for primary melanoma cells. In spheroid cultures (3D), we observed nine significant chemical shifts, of which eight markers were specific for primary melanoma spheroids, whereas the other one marker was specific to metastatic melanoma spheroids. This study suggests that the glucose accumulation and phospholipid composition vary significantly between the primary and metastatic cells lines that are obtained from a single donor and also with the cell culturing methods. 14 qualitative chemical shift markers were obtained in the comparison between monolayer culture and spheroids cultures irrespective of the differences in the cell lines. Among which 4 were unique to monolayer cultures whereas 10 chemical shifts were unique to the spheroid cultures. This study also shows that the method of cell culture would drastically affect the phospholipid composition of the cells and also depicts that the cells in spheroid culture closely resembles the cells in vivo.ConclusionThis study shows the high specificity of proton NMR spectrometry in characterizing cancer cell lines and also shows the variations in the glucose accumulation and phospholipid composition between the primary and metastatic melanoma cell lines from the same donor. Differences in the cell culture method does plays an important role in phospholipid composition of the cells.Electronic supplementary materialThe online version of this article (doi:10.1186/s40659-017-0117-8) contains supplementary material, which is available to authorized users.

show abstract

Analysis of mixed lipid extracts using ¹H NMR spectra

Cited by 6 publications

References 0 publications

Design and characterization of a new drug nanocarrier made from solid–liquid lipid mixtures

Design and characterization of a new drug nanocarrier made from solid–liquid lipid mixtures

A robust method for performing 1H-NMR lipidomics in embryonic zebrafish

Proton NMR characterization of intact primary and metastatic melanoma cells in 2D & 3D cultures

Contact Info

Product

Resources

About

Analysis of mixed lipid extracts using 1H NMR spectra

Cited by 6 publications

References 0 publications

Design and characterization of a new drug nanocarrier made from solid–liquid lipid mixtures

Design and characterization of a new drug nanocarrier made from solid–liquid lipid mixtures

A robust method for performing 1H-NMR lipidomics in embryonic zebrafish

Proton NMR characterization of intact primary and metastatic melanoma cells in 2D & 3D cultures

Contact Info

Product

Resources

About

Analysis of mixed lipid extracts using ¹H NMR spectra