Analysis of mixtures of fatty acids and fatty alcohols in fermentation broth

Liu, Yilan; Cui, Ting; Yang, Maohua; Wang, Caixia; Huo, Weiyan; Yan, Daojiang; Chen, Jinjin; Zhou, Jiemin; Xing, Jianmin

doi:10.1016/j.chroma.2013.10.094

Cited by 9 publications

(3 citation statements)

References 21 publications

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“…The analysis of fatty acids and fatty alcohols was performed via HPLC with an Agilent 1200 (Agilent, Co. Ltd. USA) equipped with RID and a SilGreen ODS C18 column (4.6 mm × 250 mm, 5 μm) according to the reported research [ 34 ]. The mobile phase was methanol: water: acetic acid (90:9.9:0.1, v/v/v).…”

Section: Methodsmentioning

confidence: 99%

High production of fatty alcohols in Escherichia coli with fatty acid starvation

Liu

Chen

et al. 2016

Microb Cell Fact

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BackgroundMicrobial biofuel synthesis attracting increasing attention. Great advances have been made in producing fatty alcohols from fatty acyl-CoAs and fatty acids in Escherichia coli. However, the low titers and limited knowledge regarding the basic characteristics of fatty alcohols, such as location and toxicity, have hampered large-scale industrialization. Further research is still needed.ResultsIn this study, we designed a novel and efficient strategy to enhance fatty alcohol production by inducing fatty acid starvation. We report the first use of deletions of acyl-ACP thioesterases to enhance fatty alcohol production. Transcriptional analysis was conducted to investigate the mechanism of the designed strategy. Then, fatty alcohol production was further enhanced by deletion of genes from competing pathways. Fatty alcohols were shown to be extracellular products with low toxicity. The final strain, E. coli MGL2, produced fatty alcohols at the remarkable level of 6.33 g/L under fed-batch fermentation, representing the highest reported titer of fatty alcohols produced by microorganisms.ConclusionsDeletions of genes responsible for synthesis of fatty acids and competing products are promising strategies for fatty alcohol production. Our investigation of the location and toxicity of fatty alcohols suggest bright future for fatty alcohol production in E. coli.Electronic supplementary materialThe online version of this article (doi:10.1186/s12934-016-0524-5) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

High production of fatty alcohols in Escherichia coli with fatty acid starvation

Liu

Chen

et al. 2016

Microb Cell Fact

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“…The resulting PCR products were confirmed by sequencing. In addition, HPLC analysis was conducted in MGKFSFS and MGFARINS according to the published method [ 36 ]. As shown in Fig 3B , fatty alcohol production was increased from 10 mg/L to 61 and 72 mg/L, respectively.…”

Section: Resultsmentioning

confidence: 99%

PCR-Based Seamless Genome Editing with High Efficiency and Fidelity in Escherichia coli

et al. 2016

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Efficiency and fidelity are the key obstacles for genome editing toolboxes. In the present study, a PCR-based tandem repeat assisted genome editing (TRAGE) method with high efficiency and fidelity was developed. The design of TRAGE is based on the mechanism of repair of spontaneous double-strand breakage (DSB) via replication fork reactivation. First, cat-sacB cassette flanked by tandem repeat sequence was integrated into target site in chromosome assisted by Red enzymes. Then, for the excision of the cat-sacB cassette, only subculturing is needed. The developed method was successfully applied for seamlessly deleting, substituting and inserting targeted genes using PCR products. The effects of different manipulations including sucrose addition time, subculture times in LB with sucrose and stages of inoculation on the efficiency were investigated. With our recommended procedure, seamless excision of cat-sacB cassette can be realized in 48 h efficiently. We believe that the developed method has great potential for seamless genome editing in E. coli.

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“…Excellent medicinal properties of Ganoderma are therefore enhanced with the low-molecular components of fatty-acid nature in mycelium (Lin et al, 2009). Microbial production of fatty acids and fatty alcohols has attracted increasing concern because of the energy crisis and environmental impact of fossil fuels (Liu et al, 2014). Therefore, efficient and simple methods for the producers' (mushroom) culture and these compounds' extraction and quantification become necessary.…”

Section: Evaluation Of Fatty Acids In Submerged Myceliamentioning

confidence: 99%

Vietnamese Ganoderma: growth, peculiarities, and low-molecular composition compared to European and Siberian strains

Tsivileva¹,

Nguyen²,

Ву³

et al. 2016

Turk J Bot

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The species from the genus Ganoderma are known to be extremely important macrofungi in fundamental biological, medicinal, and socioeconomic aspects. The present communication describes the brief morphological description, macro-and microscopic details, and chemical constituents of the representatives of five species of Ganoderma mushroom collected from different sites in Vietnamese National Parks. These species were Ganoderma colossus, G. neojaponicum, G. cattienensis, G. lucidum, and G. applanatum. Three additional herbarium strains of Ganoderma from European and Siberian regions have been implemented in the present framework for the purposes of comparison. Cultural characterization on solid and liquid fermentation, and scanning electron microscopy of morphology along with chemical analysis served as the supporting identification and comparison factors. Low-molecular-weight chemical constituents were evaluated by gas chromatography-mass spectrometry and gas-liquid chromatography techniques. Valuable substances (2-monolinolein, 2,3-dihydroxypropyl elaidate, fatty alcohol, fatty acid alkyl esters, and free fatty acids) detected in pigmented mycelia and submerged cultures have promising biotechnological applications including in food supplements, lipid-based drug delivery systems, and biodieselrelated items. The representative voucher specimens were deposited at the Herbarium of the Southern Institute of Ecology (Ho Chi Minh City, Vietnam) and were assigned accession numbers.

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Analysis of mixtures of fatty acids and fatty alcohols in fermentation broth

Cited by 9 publications

References 21 publications

High production of fatty alcohols in Escherichia coli with fatty acid starvation

High production of fatty alcohols in Escherichia coli with fatty acid starvation

PCR-Based Seamless Genome Editing with High Efficiency and Fidelity in Escherichia coli

Vietnamese Ganoderma: growth, peculiarities, and low-molecular composition compared to European and Siberian strains

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