Analysis of mixtures of polycyclic aromatic hydrocarbons in sea-water by synchronous fluorescence spectrometry in organized media

Abstract

The spectrofluorimetric characteristics of chrysene, perylene, dibenz [a,c]anthracene and coronene were studied in aqueous medium and in the presence of cationic, anionic and non-ionic surfactants. The great enhancement of the fluorescence of these polycyclic aromatic hydrocarbons (PAHs) i n hexadecyltrimethylammonium bromide and synchronous fluorescence spectrometry were used for the establishment of two methods for the analysis of binary and ternary mixtures of these compounds. The former allows the simultaneous determination of dibenz[a,c]anthracene and coronene using Ah = 89 nm, with detection limits of 0.20 and 0.22 ng ml-l, respectively. In the latter, three different Ah values are used for the simultaneous determination of chrysene, perylene and coronene, viz., 41,3 and 140 nm, respectively. Limits of detection of 0.17 ng ml-I for chrysene, 0.13 ng ml-I for perylene and 0.14 ng ml-I for coronene were obtained. Good recoveries were obtained when both methods were applied t o the analysis of binary and ternary mixtures of these PAHs added to sea-water.

EPR and Fluorescence Spectroscopy in the Photodegradation Study of Arabian and Colombian Crude Oils

“…The development of SFS-based methods for the analysis of PAHs (polycyclic aromatic hydrocarbons) in solution without a preseparation procedure has been a topic of interest in the past few years. The SFS characteristics of chrysene, perylene, dibenz[a,c]anthracene, and coronene were studied in aqueous and aqueous micellar media [33]. Synchronous fluorescence methods were used to determine selected polynuclear aromatic hydrocarbons in a coal liquid (solvent-refined coal) product without a preseparation step [34].…”

Applications and New Developments in Fluorescence Spectroscopic Techniques for the Analysis of Polycyclic Aromatic Hydrocarbons

“…A constant scan rate between two monochromators gives a constant wavelength interval (Á) between em and ex , which is known as constant wavelength SFS. Because of the simplicity and commercial availability of the required software for simultaneous scan, this technique is more popular for PAHs analysis in their complex mixture, [33] in micellar medium, [34][35] in petroleum products [36] and in aromatic fraction of low temperature tar from hard coal. [37] Derivative SFS technique is useful to resolve overlapping between PAHs spectra.…”

Screening Pyrene Metabolites in the Hemolymph of Dungeness Crabs (Cancer Magister) With Synchronous Fluorescence Spectrometry: Method Development and Application

“…Ariese et al [4] first used synchronous fluorescence spectrometry (SFS) as a biomonitoring tool to screen for pyrene-1-glucuronide in fish bile. In recent years, SFS also has been used increasingly to screen for PAHs in various sample matrices including seawater [5], fish bile [4,6,7], terrestrial isopods [8], and crab hemolymph [6,9]. A spectrofluorometric assay may be conducted either by scanning both the excitation and emission monochromator of a spectrofluorometer simultaneously with a constant wavelength difference (the SFS approach) or by scanning the emission monochromator and keeping the excitation wavelength fixed (the fixed-wavelength approach) [10].…”