Analysis of molecular alterations in chromosome 8 associated with the development of uterine cervical carcinoma of Indian patients

Bhattacharya, Nilanjana; Singh, Ratnesh; Mondal, S. S.; Roy, Anup; Mondal, Ranajit Kumar; Roychowdhury, Susanta; Panda, Chinmay Kumar

doi:10.1016/j.ygyno.2004.07.036

Cited by 13 publications

(11 citation statements)

References 40 publications

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“…To confirm the HPV types, the PCR products in the agarose gels were transferred to a gene screen nylon membrane for Southern hybridization using 32 P-labeled HPV type-specific probes (13).…”

Section: Determination Of Hpv Typesmentioning

confidence: 99%

Prevalence of Human Papillomavirus in Women Without Cervical Cancer

Dutta

Begum

et al. 2012

International Journal of Gynecological Pathology

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Despite the high incidence of cervical cancer, population-based data on prevalence of human papillomavirus (HPV) are limited in India. This study aimed to evaluate the prevalence of any HPV type and type-specific prevalence of HPV 16/18 in women without cervical cancer. HPV viral load was measured and correlated with cytologic abnormalities of the cervix. A total of 2501 women between 25 and 65 years of age and without cervical cancer were screened by pap smear cytology. HPV DNA was detected from cervical scrapes by nested polymerase chain reaction. Detection of HPV 16/18 was carried out by polymerase chain reaction using type-specific primers and was confirmed by Southern hybridization. Viral load was determined by absolute real-time polymerase chain reaction. Population prevalence of any HPV was found to be 9.9%. The risk of HPV infection was higher in women aged 25 to 34 years (odds ratio, 1.11), in married women below 20 years of age (odds ratio, 1.80), and in women with parity ≥4 (odds ratio, 1.04). Prevalence of HPV 18 (1.4%) was greater than that of HPV 16 (0.6%) in the overall screened population. High-grade squamous intraepithelial lesion cytology was more frequent in women infected with HPV 16 than in those infected with HPV 18 and other types. A gradual increase in HPV copy numbers was associated with progressive cytologic severity. In this study, HPV prevalence is comparable to HPV prevalence reported by other studies among Indian and Asian women. Although the prevalence of HPV 18 was more than that of HPV 16, type 16 infection was associated with higher oncogenicity.

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Section: Determination Of Hpv Typesmentioning

confidence: 99%

Prevalence of Human Papillomavirus in Women Without Cervical Cancer

Dutta

Begum

et al. 2012

International Journal of Gynecological Pathology

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“…HindIII-digested genomic DNA of 52 cervical lesions and corresponding normal tissues was electrophoresed in 0.8% agarose gel, transferred to GeneScreen nylon membrane (NEN, USA), hybridized with the P 32 -labeled probes ITGA9, RBSP3, and CD3-gamma (Supporting Information Table S1) separately, and autoradiographed (Kashuba et al, 1999;Bhattacharya et al, 2004;Sulimova et al, 2005). The intensities of ITGA9/ RBSP3/CD3gamma bands were determined by densitometric scanning (Bio-Rad GS-800, USA) and allelic status was calculated there from (Ichimura et al, 2000) (Supporting Information).…”

Section: Physical Mapping Of Itga9 and Rbsp3 Loci By Southern Blot Anmentioning

confidence: 99%

RBSP3 is frequently altered in premalignant cervical lesions: Clinical and prognostic significance

Mitra

Indra

Bhattacharya

et al. 2009

Genes Chromosomes & Cancer

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To understand the importance of frequent deletion of 3p22.3 in cervical carcinogenesis, alterations (deletion/methylation/expression) of the candidate genes STAC, MLH1, ITGA9, and RBSP3, located in the region, were analyzed in 24 cervical intraepithelial neoplasia (CIN) and 137 uterine cervical carcinoma (CACX) samples. In CIN, RBSP3 deletion (48%) and methylation (26%) were high compared with the other genes (4-9%). In CACX, alterations of these genes were as follows: deletion: STAC (54%) > MLH1 (46%) > RBSP3 (45%) > ITGA9 (41%), methylation: RBSP3 (25%) > ITGA9 (24%) > STAC (19%) > MLH1 (13%). Overall, alterations of RBSP3 showed association with CIN, whereas for STAC and MLH1, this frequency increased significantly from CIN --> Stage I/II and for ITGA9 from CIN --> Stage I/II and also from Stage I/II --> Stage III/IV. Quantitative mRNA expression analysis showed differential reduced expression of these genes in CACX concordant to their molecular alterations. The more active RBSP3B splice variant was underexpressed in CACX. RB1 was infrequently deleted in CACX. Concordance was seen between (i) inactivation of RBSP3 and intense p-RB1 nuclear immunostaining and (ii) low/absence of MLH1 expression and its molecular alterations in CACX. In normal cervical epithelium, p-RB1 immunostaining was low in differentiated cells, whereas MLH1 staining was seen in both nucleus and cytoplasm irrespective of differentiation stage. Alterations of the genes were significantly associated with poor prognosis. High parity (>or=5)/early sexual debut (

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“…2 It is estimated that about 132,082 women die due to cervical cancer every year, accounting for 26.7% of the worldwide incidence. 1 Cervical cancer ICD-10 category C53 3 is one of the common neoplastic diseases affecting women.…”

Section: Introductionmentioning

confidence: 99%