Analysis of molecular markers as IL-12, IL-22 and IFN-γ in correlation with a clinical course in patients with psoriasis

Abstract

As a chronic, recurrent, immunologically mediated systemic disease and a common cause of dermatological problems, psoriasis is often a subject of scientific research. Skin changes located on the hands can cause difficulties and limitations in the performance of professional activities, especially manual ones. The main role in pathogenesis is played by immunological factors-improper functioning of the components of the immune system, among others, T lymphocytes and cytokines like interleukin-12 (IL-12), interleukin-22 (IL-22) and interferon gamma (IFN-γ). Material and Methods: The obtained tissue and blood were destined for RNA isolation. The RNA was then subjected to a reverse transcription reaction. The relative gene expression level was evaluated by the real-time polymerase chain reaction for IL-12B, IL-22 and IFN-γ genes, and presented as the relative quantification (RQ) value, relative to the reference gene GAPDH. In addition, a correlation analysis of the expression level of selected genes with the clinical course of the disease, as assessed by the Psoriasis Area and Severity Index (PASI), the Body Surface Area (BSA) and the Dermatology Life Quality Index (DLQI) scores was performed. Results: Statistical analysis confirmed a significant increase in RQ values for IL-12B, IL-22 and IFN-γ in the group of psoriatic patients vs. the control group. A positive correlation was also found between BSA and PASI and RQ for the IL-12B gene. Conclusions: Increased expression levels of IL-12B, IL-22 and IFN-γ genes in psoriatic skin confirm that selected cytokines play an important role in the initiation and sustenance of psoriasis.

An Analysis of IL-10, IL-17A, IL-17RA, IL-23A and IL-23R Expression and Their Correlation with Clinical Course in Patients with Psoriasis

“…Isolation of total RNA from T-regulatory cells and skin homogenates was performed using the mirVana™ miRNA Isolation Kit (Life Technologies, Carlsbad, CA, USA), which provides efficient isolation of small RNA-containing total RNA. This kit is compatible with virtually all cell and tissue types, and has been used previously to isolate RNA from body fluids and tissues [42][43][44]. Isolation of total RNA was carried out following the manufacturer's recommendations.…”

“…The evaluation of gene expression was performed analogously to the previously described procedure [44]. The reverse transcription (RT) reaction was performed using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Carlsbad, CA, USA) in a volume of 20 µL.…”

“…In the present study, IL-23A gene expression was decreased in the psoriasis group and the controls. This may be because the expression of p40 subunit, which is shared by IL-23 and IL-12, can be a key factor in the development and maintenance of psoriatic skin lesions: previous studies report elevated level of gene expression of IL-12B in diseased skin [44,109,110]. Different results were presented by Fitch et al [71], Chan et al [80], Lee et al [108] and Chamian et al [111], who reported an increase in p19 mRNA in psoriatic skin compared with healthy skin, as well as an elevated level of p40 in lesional tissues, but not p35, which suggests IL-23 plays a greater role in the pathogenesis of psoriasis than IL-12.…”

Cytokine Profiles and the Relationship of Disease Severity in Patients with Psoriasis

“…Related research showed that immune and inflammatory mechanisms play essential roles in psoriatic pathogenesis. [ 15 ] Especially, pathogenic T cell subsets such as Th1, Th17, Th22 and their characteristic cytokines including IFN-γ, IL-1β, IL-6, TNFα, IL-17, IL-23, chemokines and so on form the immune network of psoriasis through complex signal transduction.…”

Etiopathogenesis of Psoriasis from Genetic Perspective: An updated Review

“…The effect of IL-22 on keratinocytes was highlighted through overexpression of IL-22 receptor (IL-22R) in the epidermis of psoriatic skin compared to that of normal skin [ 120 ]. Studies revealed that upregulation of IL-22, along with IL-12B and IFN-γ in the psoriatic skin, is involved in the initiation and sustenance of the disease [ 121 ]. A GWAS identified the IL-22 association with psoriasis in the Japanese [ 122 ].…”