Analysis of Molecular Variance and Population Structure of Sesame (<i>Sesamum indicum</i>L.) Genotypes Using Simple Sequence Repeat Markers

Asekova, Sovetgul; Kulkarni, Krishnanand P.; Oh, Ki Won; Lee, Myung‐Hee; Oh, Eunyoung; Kim, Jung-In; Yeo, Un-Sang; Pae, Suk-Bok; Ha, Tae Joung; Kim, Sung Up

doi:10.9787/pbb.2018.6.4.321

Cited by 12 publications

(24 citation statements)

References 34 publications

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“…The mean observed heterozygosity of 0.43 reported on the present study is lower than the value of 0.56 reported by [25] when assessing 25 sesame specimens using 21 SSR markers. The observed heterozygosity in this study was higher than values of 0.23, 0.01, and 0.12 reported by [19,21,22] when assessing 50, 129 and 36 sesame specimens using 10, 23 and 10 SSR markers, respectively. The mean expected heterozygosity (He = 0.30) recorded in the present study (Table 5) was lower than values of 0.72 and 0.34 reported by [21,22] when evaluating 129 and 36 sesame accessions using 23 and 10 SSR markers, respectively.…”

Section: Traits Associationscontrasting

confidence: 83%

“…The observed heterozygosity in this study was higher than values of 0.23, 0.01, and 0.12 reported by [19,21,22] when assessing 50, 129 and 36 sesame specimens using 10, 23 and 10 SSR markers, respectively. The mean expected heterozygosity (He = 0.30) recorded in the present study (Table 5) was lower than values of 0.72 and 0.34 reported by [21,22] when evaluating 129 and 36 sesame accessions using 23 and 10 SSR markers, respectively. The higher heterozygosity recorded in the present study suggested that Ethiopian sesame population have high level of genetic variation for selection.…”

Section: Traits Associationscontrasting

confidence: 83%

“…Most released entries (Humera-1, Setit-1 and Setit-3) were grouped in sub-population 4. [20,21] reported two and three populations among 94 and 129 sesame accessions sampled from China and Korea collections and when assessed with 44 and 23 SSR markers. The higher gene xation index of 0.39 for population I which comprised of accessions collected from Amhara, Tigray, Afar, and Oromia regions suggested higher genetic differentiation attributable to high gene ow among these regions.…”

Section: Traits Associationsmentioning

confidence: 99%

“…2.). [21] grouped 129 sesame specimens into two clusters using 23 SSR markers. In the present study, the clustering patterns of the specimens did not correspond to the prede ned population structure based on the regions of collection.…”

Section: Traits Associationsmentioning

confidence: 99%

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Genetic Variability and Population Structure of Ethiopian Sesame (Sesamum Indicum L.) Germplasm Assessed Through Phenotypic Traits and Simple Sequence Repeats Markers

Teklu

Shimelis

Tesfaye

et al. 2021

Preprint

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Background Ethiopia is one of the centres of genetic diversity of sesame (Sesamum indicum L.). The sesame genetic resources present in the country should be explored for local, regional and international sesame improvement programs to design high performing and market preferred varieties. This study's objectives were to determine the extent of genetic variation among 100 diverse cultivated sesame germplasm collections of Ethiopia using phenotypic traits and simple sequence repeat (SSR) markers to select distinct and complementary specimens for breeding. One-hundred sesame entries were field evaluated at two locations in Ethiopia for agro-morphological traits and seed oil content using a 10 × 10 lattice design with two replications. Test specimens were profiled using 27 selected polymorphic SSR markers. Results The analysis of variance revealed significant (P ≤ 0.05) entry by environment interaction for plant height, internode length, number of secondary branches, and seed yield. Genotypes such as Hirhir Kebabo Hairless-9, Setit-3, Orofalc ACC-2, Hirhir Humera Sel-6, ABX=2-01-2, and Setit-1 recorded higher grain yield of > 0.73 ton ha-1 with excellent performance in yield component such as oil and seed yield per hectare. Seed yield had positive and significant (p < 0.01) associations with oil yield (r = 0.99) useful for simultaneous selection for yield improvement in sesame. The SSR markers revealed gene diversity and polymorphic information content of 0.30 and 0.25, respectively, showing that the tested sesame accessions were genetically diverse. Cluster analysis resolved the accessions into two groups, while population structure analysis revealed four major heterotic groups, this enabling selection and subsequent crosses to develop breeding populations for cultivar development.Conclusions Based on phenotypic and genomic divergence, the following complementary specimens were selected: Hirhir Humera Sel-6, Setit-3, Hirhir Kebabo Hairless Sel-4, Hirhir Nigara 1st Sel-1, Humera-1 and Hirhir Kebabo Early Sel-1 (from cluster II-a), Hirhir kebabo hairless-9, NN-0029(2), NN0068-2 and Bawnji Fiyel Kolet, (from cluster II-b). The selected genetic resources are recommended for use in sesame production and breeding programs in Ethiopia.

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Section: Traits Associationscontrasting

confidence: 83%

Section: Traits Associationscontrasting

confidence: 83%

Section: Traits Associationsmentioning

confidence: 99%

Section: Traits Associationsmentioning

confidence: 99%

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Genetic Variability and Population Structure of Ethiopian Sesame (Sesamum Indicum L.) Germplasm Assessed Through Phenotypic Traits and Simple Sequence Repeats Markers

Teklu

Shimelis

Tesfaye

et al. 2021

Preprint

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“…Hence, there is a need to assess the genetic diversity among the present sesame cultivars and evaluate them for the resistance to P. nicotianae . The genetic diversity and population structure analysis may help identify the genetically divergent varieties which can be used for breeding purpose (Asekova et al, 2018). Such sources are used to identify the genetic loci (QTLs or markers linked to the trait) responsible for the resistance to P. nicotianae .…”

Section: Introductionmentioning

confidence: 99%

A Combinatorial Approach of Biparental QTL Mapping and Genome-Wide Association Analysis Identifies Candidate Genes for Phytophthora Blight Resistance in Sesame

Asekova

Kulkarni

et al. 2020

Preprint

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SummaryPhytophthora blight, caused by pathogen Phytophthora nicotianae, is responsible for a huge reduction in sesame (Sesamum indicum L.) crop yields. In this study, we utilized a combinatorial approach involving biparental QTL mapping and genome-wide association (GWAS) analysis to identify genes associated with Phytophthora blight resistance in sesame. Evaluation of resistant of the parental varieties (Goenbaek, Osan and Milsung) and the RILs of both the populations in greenhouse conditions suggested the qualitative nature of the trait.. The genetic map comprised thirteen LGs covering a total map length of 887.49 cM with an average inter-marker distance of 4.69 cM. Significant QTLs explaining phenotypic variation in the range of 2.25% to 69.24% were identified on chromosomes 10 and 13 (Chr10 and Chr13). A resistance locus detected on Chr10 was found to be highly significant. The association of this locus to PBR was also identified through BSA and single marker analysis in Goenbaek × Milsung cross and through genome-wide association mapping of 87 sesame accessions. The GWAS analysis identified 44 SNP loci significantly associated with Phytophthora disease-resistant traits on Chr10. Further, the haplotype block analysis conducted in order to find whether the SNPs associated with resistance in this study showed that the SNPs are in high LD with the resistance QTL. We obtained a total of 68 candidate genes, which included a number of defense-related R genes. One of the genes, SIN_1019016 (At1g58390) showed high expression in the resistant parent. The results from this study would be highly useful in identifying genetic and molecular factors associated with Phytophthora blight resistance in sesame.

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Research advances and prospects of molecular markers in sesame: a review

Weldemichael,

Gebremedhn

2023

Plant Biotechnol Rep

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Analysis of Molecular Variance and Population Structure of Sesame (Sesamum indicumL.) Genotypes Using Simple Sequence Repeat Markers

Cited by 12 publications

References 34 publications

Genetic Variability and Population Structure of Ethiopian Sesame (Sesamum Indicum L.) Germplasm Assessed Through Phenotypic Traits and Simple Sequence Repeats Markers

Genetic Variability and Population Structure of Ethiopian Sesame (Sesamum Indicum L.) Germplasm Assessed Through Phenotypic Traits and Simple Sequence Repeats Markers

A Combinatorial Approach of Biparental QTL Mapping and Genome-Wide Association Analysis Identifies Candidate Genes for Phytophthora Blight Resistance in Sesame

Research advances and prospects of molecular markers in sesame: a review

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