Analysis of molecular variation among diverse background wheat (Triticum aestivum L) genotypes with the help of ISSR markers

Abstract

Inter Simple Sequence Repeat (ISSR) provide adequate information about polymorphism and reproducible fingerprinting profiles for evaluating heritable diversity in different crops including wheat. The main objective of this study was to reveal the genetic diversity and relationships among different wheat genotypes. Genomic DNA from twenty wheat genotypes were analyzed using 25 inter simple sequence repeats (ISSR) markers. Out of 25 ISSR primers, 16 showed high level of polymorphism and 9 primers were monomorphic in nature. 1.66 bands per primer were amplified on an average. The PIC value varied from 0.048 to 0.747 for 25 primers. RP value of 25 ISSR primers ranged from 0.95-1.96 with an average of 1.57. The primer UBC 814 was reported as best one in distinguishing the 20 durum varieties. Similarity values for all the 20 genotypes ranges from 0.087 to 0.537 showing substantial differences among the wheat varieties. The 25 ISSR markers were used to construct the dendrogram based on UPGMA cluster analysis. The dendrogram indicates that the ISSR markers succeeded in distinguishing most of 20 varieties in relation to their genetic background and their geographical origin.

Revealing Genetic Diversity and Population Structure in Türkiye’s Wheat Germplasm Using iPBS-Retrotransposon Markers

“…In this investigation, the mean PIC value was determined to be 0.22. While this value is comparatively lower than those reported in other studies utilizing distinct marker systems [11,[43][44][45]47], the mean PIC value obtained in our research exceeds the value published by Khaled et al [48]. The observed variance in PIC values could potentially be attributed to the use of different marker systems or the presence of a variable number of genotypes and markers in the study [49].…”

“…In this study, an average of 16.3 polymorphic bands were obtained using 10 iPBS markers. On the other hand, the mean polymorphic bands that were reported in this study were higher than those that were reported by Nazarzadeh et al [22] using RAPD and ISSR markers, Kumar et al [43] using ISSR markers, Alshehri et al [44] using SCoT and ISSR primers, Çifçi and Ya gdi [45] using RAPD markers, Nadeem [11] using iPBS marker, and Abbasi Holasou et al [41] using IRAP and REMAP markers.…”