Analysis of mRNA and MicroRNA Expression Profiles of Nervous Tissues and Reproductive Tissues in Male Procambarus clarkii After Silencing IAG

Sun, Rong; Yi, Shaokui; Shi, Linlin; Tan, Kianann; Shi, Ruixue; Yang, Siqi; Li, Yanhe

doi:10.1007/s10126-023-10254-z

Cited by 2 publications

(1 citation statement)

References 48 publications

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“…In M. rosenbergii, MrDsx expression was significantly induced in the testis and AG following eyestalk ablation, and gene knockdown of MrDsx resulted in a significant decrease in MrIAG transcript levels in the AG, suggesting that eyestalk elements negatively control MrDsx, which regulates the activation of MrIAG [77]. As part of the extensive research aimed at elucidating the IAG signaling cascade in decapod species, IAG loss-of-function studies have played a significant role in the understanding of the IAG downstream factors [78][79][80][81][82][83]. Still, in most of the studies on candidates upstream of the IAG-switch, it seems that the intervention (usually RNAi) is too late to effectively influence sexual differentiation.…”

Section: Controlling Elements Of the Iag-switchmentioning

confidence: 99%

Monosex Populations of the Giant Freshwater Prawn Macrobrachium rosenbergii—From a Pre-Molecular Start to the Next Generation Era

Wahl,

Levy,

Ventura

et al. 2023

IJMS

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Sexual manipulation in the giant freshwater prawn Macrobrachium rosenbergii has proven successful in generating monosex (both all-male and all-female) populations for aquaculture using a crustacean-specific endocrine gland, the androgenic gland (AG), which serves as a key masculinizing factor by producing and secreting an insulin-like AG hormone (IAG). Here, we provide a summary of the advancements from the discovery of the AG and IAG in decapods through to the development of monosex populations in M. rosenbergii. We discuss the broader sexual development pathway, which is highly divergent across decapods, and provide our future perspective on the utility of novel genetic and genomic tools in promoting refined approaches towards monosex biotechnology. Finally, the future potential benefits of deploying monosex prawn populations for environmental management are discussed.

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Section: Controlling Elements Of the Iag-switchmentioning

confidence: 99%

Monosex Populations of the Giant Freshwater Prawn Macrobrachium rosenbergii—From a Pre-Molecular Start to the Next Generation Era

Wahl,

Levy,

Ventura

et al. 2023

IJMS

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A study on the functional role of the DHCR24 gene in gonadal differentiation and development of Macrobrachium nipponense

Cai,

Zhang,

Jiang

et al. 2024

Sci Rep

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Sex differentiation in crustaceans is a complex process influenced by various factors, including the androgenic gland and sex-related genes. This study characterized the role of the Mn-DHCR24 gene in the oriental river prawn ( Macrobrachium nipponense ). We used bioinformatics to analyze sequence features and phylogenetic relationships of a single Mn-DHCR24 gene. The expression patterns of Mn-DHCR24 across different tissues and developmental stages were determined by real-time PCR, and its localization in testis was determined by in situ hybridization. RNA interference was used to knock down Mn-DHCR24 expression, followed by examining changes in sex ratio and gonadal development at the PL10 stage. Additionally, an enzyme-linked immunosorbent assay measured 17α-methyltestosterone levels, and tissue sections were used to characterize gonadal development. The results indicated that Mn-DHCR24 was high expression in testis, which was critical for sperm maturation and gonadal differentiation. RNAi experiments showed the role of Mn-DHCR24 during reproductive regulation rather than as a master gene for sex differentiation. This study further showed that Mn-DHCR24 regulated sex and hormone-related genes, influencing steroid biosynthesis pathways. Together, these findings provided valuable insights into the genetic and hormonal mechanisms of gonadal differentiation in M. nipponense , and supported the development of monosex culture technology.

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Analysis of mRNA and MicroRNA Expression Profiles of Nervous Tissues and Reproductive Tissues in Male Procambarus clarkii After Silencing IAG

Cited by 2 publications

References 48 publications

Monosex Populations of the Giant Freshwater Prawn Macrobrachium rosenbergii—From a Pre-Molecular Start to the Next Generation Era

Monosex Populations of the Giant Freshwater Prawn Macrobrachium rosenbergii—From a Pre-Molecular Start to the Next Generation Era

A study on the functional role of the DHCR24 gene in gonadal differentiation and development of Macrobrachium nipponense

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