Analysis of mRNA expression for interleukin-1 genes on human testicular cells

Janitz, Michał; Fiszer, Dorota; Lukaszyk, A; Skorupski, Witold; Kurpisz, Maciej

doi:10.1016/0165-2478(95)02458-1

Cited by 8 publications

(4 citation statements)

References 21 publications

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“…These data are in accordance with previous results obtained in the rat showing that these cytokines are produced by Sertoli cells (11,17,18) and Leydig cells (9,10,20,21) and the recent finding of IL-1 mRNA in crude human testicular cell preparations (41). These mRNAs are translated into proteins that can be detected in culture media by bioassays and immunoassays.…”

Section: Discussionsupporting

confidence: 93%

Human Leydig Cells and Sertoli Cells Are Producers of Interleukins-1 and -6¹

Cudicini

Lejeune

Gomez

et al. 1997

The Journal of Clinical Endocrinology & Metabolism

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Interleukins (IL)-1 and -6 have been shown to be produced by several categories of cells in the rat testis and involved in the paracrine control of testicular function. Evidence of high amounts of IL-1 have been shown in the human testis, but nothing is known about its cellular origin. Furthermore, to our knowledge, the presence of IL-6 in the human testis has not yet been investigated. Therefore, the present study was aimed at identifying IL-1 and -6 expression and production within the human testis, using RT-PCR, bioassays, and enzyme linked immunosorbent assays. We demonstrated that IL-1 and -6 messenger RNA and proteins were produced constitutively in vitro by human Leydig cell- and Sertoli cell-enriched preparations. FSH only stimulated IL-6 production by Sertoli cell-enriched preparations, but increased the release of both IL-1 and -6 in germ cell-depleted Sertoli cell cultures. In addition, lipopolysaccharides and latex beads enhanced the production of both cytokines by Sertoli cell cultures, whereas human chorionic gonadotropin and lipopolysaccharides enhanced the release of both cytokines by Leydig cells. Enzyme linked immunosorbent assays and neutralization experiments revealed that human Sertoli cells produce essentially the alpha form of IL-1, whereas both forms, alpha and beta, are present in Leydig cells. The demonstration that human Leydig and Sertoli cells produce IL-1 and -6 under the control of gonadotropin hormones and exogenous factors, opens the possibility to study the involvement of these cytokines in the control of testis function, in normal and pathological conditions in men.

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Section: Discussionsupporting

confidence: 93%

Human Leydig Cells and Sertoli Cells Are Producers of Interleukins-1 and -6¹

Cudicini

Lejeune

Gomez

et al. 1997

The Journal of Clinical Endocrinology & Metabolism

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“…In our study, we have shown the intense expression of IL-1b gene in the interstitial cells (Figure 1), earlier observed in our previous work. 16 Numerous studies, reported an inhibitory role of IL-1 on testosterone production, whereas in another scenario this cytokine appeared as a growth factor for immature Leydig cells. 17 It was also shown that Leydig cells express both Fas and FasL without markers of visible apoptosis whereas the other groups demonstrated that FasL induced production and secretion of IL-1b.…”

Section: Discussionmentioning

confidence: 99%

Interleukin-1 superfamily genes expression in normal or impaired human spermatogenesis

et al. 2007

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Interleukin-1 (IL-1) is a pleiotropic cytokine that may play a role in contributing to the specific immune environment of mammalian testis and in regulating cell differentiation. We have determined the transcription activity of the IL-1 gene family (using real-time polymerase chain reaction (PCR)) in two main functional testicular compartments (interstitial and intratubular ones), and in tissue homogenates obtained from patients with fertility disorders (spermatogenic arrest and testicular tumors). We observed the prominent expression of gene coding for IL-1 receptor antagonist (IL-1RA) in a purified fraction of gametogenic cells (normal gonad). Caspase-1 (ICE -IL-1b-converting enzyme) was highly expressed (on mRNA level) in interstitial compartments as well in testicular tumors (immune enhancement?). In addition we found, that the activity of IL-1RA gene decreased along spermatogenic alteration in an inversely related manner with IL-1a (from normal gonad through spermatogenic arrest to Sertoli cell only syndrome). Therefore, the quotient value of IL-1a/IL-1RA could potentially serve as the diagnostic molecular probe for spermatogenesis assessment. The precise level of mRNA for IL-1-IL-18 cytokines and their receptors, and specifically of the receptor antagonist in immune privileged gonad, could be one of the main factors responsible for maintaining testicular homeostasis, thus enabling generation of the mature spermatozoa.

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“…While the function of this variant in the testis has yet to be determined, the 24-kDa form is also capable of promoting thymocyte proliferation, but unlike the other isoforms, it fails to inhibit human chorionic gonadotropin-driven steroidogenesis in Leydig cells (interstitial cells that produce testosterone when stimulated by luteinizing hormone) [113]. In contrast, IL-1 β is absent and its transcripts are barely detectable in the testis under normal physiological conditions [38, 107, 114]. …”

Section: Role Of Il-1α In Spermatogenesismentioning

confidence: 99%

The biology of interleukin-1: emerging concepts in the regulation of the actin cytoskeleton and cell junction dynamics

Lie

Cheng

Mruk

2011

Cell. Mol. Life Sci.

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Interleukin (IL)-1 is a proinflammatory cytokine with important roles in innate immunity, as well as in normal tissue homeostasis. Interestingly, recent studies have also shown IL-1 to function in the dynamics of the actin cytoskeleton and cell junctions. For example, treatment of different epithelia with IL-1α often results in the restructuring of the actin network and cell junctions, thereby leading to junction disassembly. In this review, we highlight new and interesting findings that show IL-1 to be a critical player of restructuring events in the seminiferous epithelium of the testis during spermatogenesis.

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Analysis of mRNA expression for interleukin-1 genes on human testicular cells

Cited by 8 publications

References 21 publications

Human Leydig Cells and Sertoli Cells Are Producers of Interleukins-1 and -6¹

Human Leydig Cells and Sertoli Cells Are Producers of Interleukins-1 and -6¹

Interleukin-1 superfamily genes expression in normal or impaired human spermatogenesis

The biology of interleukin-1: emerging concepts in the regulation of the actin cytoskeleton and cell junction dynamics

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Analysis of mRNA expression for interleukin-1 genes on human testicular cells

Cited by 8 publications

References 21 publications

Human Leydig Cells and Sertoli Cells Are Producers of Interleukins-1 and -61

Human Leydig Cells and Sertoli Cells Are Producers of Interleukins-1 and -61

Interleukin-1 superfamily genes expression in normal or impaired human spermatogenesis

The biology of interleukin-1: emerging concepts in the regulation of the actin cytoskeleton and cell junction dynamics

Contact Info

Product

Resources

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Human Leydig Cells and Sertoli Cells Are Producers of Interleukins-1 and -6¹

Human Leydig Cells and Sertoli Cells Are Producers of Interleukins-1 and -6¹