“…The data-intensive scientific discoveries rely on three paradigms-theory, experimentation, and simulation modeling (Tolle et al, 2011). As big data is described with three characteristics (volume, velocity, and variety) (Stephens et al, 2015;Adil et al, 2016), data generated by SC-RNA-seq are tantamount to these three quantitative characteristics (Ivanov et al, 2013). With the introduction of new methods in microfluidics (Zare and Kim, 2010), combinatorial indexing procedures (Fan et al, 2015), and rapid drop in the sequencing cost, SC assay profiling has widely become a routine practice among biologists for analyzing millions of cells in hours, paving the way for the accumulation of a large amount of data.…”