Analysis of multiplexed short tandem repeat (STR) systems using capillary array electrophoresis

Mansfield, Edwin; Robertson, James; Vainer, Marina; Isenberg, Alice R.; Frazier, R.; Ferguson, Karin; Chow, ShiTse; Harris, Dennis W.; Barker, David; Gill, Peter; Budowle, Bruce; McCord, Bruce

doi:10.1002/elps.1150190118

Cited by 48 publications

(30 citation statements)

References 36 publications

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“…To test the resolving power of the plastic channel system, allele 9.3 of the TH01 locus was spiked to the CTTv standard ladder sample and coelectrophoresed on the channels with the 32 alleles of the standard ladder sample. Resolving allele 9.3 from allele 10 for the TH01 locus has been a commonly used assessment of single-base resolution performance for numerous STR typing electrophoresis systems, as allele 9.3 (198 bp) is one base shorter than the less common allele 10 (199 bp) [6,18,19]. As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

High‐resolution single‐stranded DNA analysis on 4.5 cm plastic electrophoretic microchannels

Shi¹,

Anderson²

2003

Electrophoresis

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Plastic microchannels (4.5 cm long) fabricated from an etched glass master were tested for high-resolution single-stranded DNA analysis. Using replaceable denaturing linear polyacrylamide as sieving matrix, one-color separation of a fragment sizing standard with single-base resolution (R > 0.5) was achieved up to 275 bases. Two-color sizing analysis of four loci short tandem repeat (STR) allelic ladder (CSF1PO, TPOX, TH01, vWA) with single-base resolution (R = 0.62) on TH01 alleles 9.3 (198 bp) and 10 (199 bp) was demonstrated. An average standard deviation of +/- 0.06 bp and +/-0.11 bp in sizing 32 alleles of the CTTv ladder was attained between runs and between channels, respectively. Four-color sequencing separation of a terminator sequencing standard showed a base-calling accuracy of 99.1% out to 320 bases in 13 min.

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Section: Resultsmentioning

confidence: 99%

High‐resolution single‐stranded DNA analysis on 4.5 cm plastic electrophoretic microchannels

Shi¹,

Anderson²

2003

Electrophoresis

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“…Genotypes from DNA amplified products were analysed in capillary gel electrophoresis using an ABI Prism 310 Genetic Analyser [6].…”

Section: Methodsmentioning

confidence: 99%

Characterisation of three Amerindian populations from Hidalgo State (Mexico) by 15 STR-PCR polymorphisms

et al. 2004

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The purpose of this study is to report allele frequency data of three ethnic Amerindian population samples: the Otomi (Hna-hnu) from eastern Sierra Madre and Ixmiquilpan valley and the Huasteco from La Huasteca. These groups were characterised by 15 STR-PCR polymorphisms (HumTH01, HumvWA, D18S51, HumTPOX, D19S433, D16S539, D13S317, D8S1179, D7S820, D5S818, HumFGA, CSF1PO, D2S1338, D3S1358 and D21S11). No significant deviations in observed allelic frequencies from Hardy-Weinberg equilibrium were found for all the studied systems. From the forensic point of view, the heterozygosity value, power of discrimination and the a priori chance of exclusion were calculated.

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“…Although tetranucleotide and pentanucleotide repeat loci are commonly used, alleles with non-standard repeat numbers may emerge by mutation, complicating the analysis. For instance, there is a relatively common 9.3 allele at locus TH01 that differs by only one base from the rarer 10 allele (Mansfield et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Efficient human paternity testing with a panel of 40 short insertion-deletion polymorphisms

Pimenta¹,

Pena²

2010

Genet. Mol. Res.

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ABSTRACT. We developed a panel of 40 multiplexed short insertiondeletion (indel) polymorphic loci with widespread chromosomal locations and allele frequencies close to 0.50 in the European population. We genotyped these markers in 360 unrelated self-classified White Brazilians and 50 mother-child-probable father trios with proven paternity. The average heterozygosity (gene diversity) per locus was 0.48, and the combined probability of identity (matching probability) for the 40-locus set was 3.48 x 10 -17. The combined power of exclusion of the indel panel was 0.9997. The efficiency of the 40 indel set in the exclusion of falsely accused individuals in paternity casework was equivalent to the CODIS set of 13 microsatellites. The geometric mean of the paternity indices of the 50 mother-child-probable father trios was 17,607. This panel of 40 short indels was found to have excellent performance. Thus, especially because of its simplicity and low cost, and the fact that it is composed of genomic markers that have very low mutation rates, it represents a useful new tool for human paternity testing.

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Analysis of multiplexed short tandem repeat (STR) systems using capillary array electrophoresis

Cited by 48 publications

References 36 publications

High‐resolution single‐stranded DNA analysis on 4.5 cm plastic electrophoretic microchannels

High‐resolution single‐stranded DNA analysis on 4.5 cm plastic electrophoretic microchannels

Characterisation of three Amerindian populations from Hidalgo State (Mexico) by 15 STR-PCR polymorphisms

Efficient human paternity testing with a panel of 40 short insertion-deletion polymorphisms

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