Analysis of Myc Bound Loci Identified by CpG Island Arrays Shows that Max Is Essential for Myc-Dependent Repression

Mao, Daniel Y.L.; Watson, John D.; Yan, Pearlly S.; Baršytė-Lovejoy, Dalia; Khosravi, Fereshteh; Wong, Wendy Wei-Lynn; Farnham, Peggy J.; Huang, Tim H.M.; Penn, Linda Z.

doi:10.1016/s0960-9822(03)00297-5

Cited by 165 publications

(154 citation statements)

References 22 publications

Supporting

Mentioning

142

Contrasting

Order By: Relevance

“…These findings suggested that gene repression contributes significantly to the phenotype of c-Myc transformed cells. Formation of Myc:Max heterodimers is essential for both Myc-dependent transcriptional activation and gene repression (Mao et al, 2003). Other combinations of c-Myc heterodimers have been described that recruit remodeling enzymes, such as HDACs, and exert a repressing effect.…”

Section: Discussionmentioning

confidence: 99%

Cyclin D1 Is Transcriptionally Down-Regulated by ZO-2 via an E Box and the Transcription Factor c-Myc

Huerta¹,

Muñoz²,

Tapia

et al. 2007

MBoC

View full text Add to dashboard Cite

Recent reports have indicated the participation of tight junction (TJ) proteins in the regulation of gene expression and cell proliferation. Here, we have studied the role of zona occludens (ZO)-2, a TJ peripheral protein, in the regulation of cyclin D1 transcription. We found that ZO-2 down-regulates cyclin D1 transcription in a dose-dependent manner. To understand how ZO-2 represses cyclin D1 promoter activity, we used deletion analyses and found that ZO-2 negatively regulates cyclin D1 transcription via an E box and that it diminishes cell proliferation. Because ZO-2 does not associate directly with DNA, electrophoretic mobility shift assay and chromatin immunoprecipitation (ChIP) assay were used to identify the transcription factors mediating the ZO-2-repressive effect. c-Myc was found to bind the E box present in the cyclin D1 promoter, and the overexpression of c-Myc augmented the inhibition generated by ZO-2 transfection. The presence of ZO-2 and c-Myc in the same complex was further demonstrated by immunoprecipitation. ChIP and reporter gene assays using histone deacetylases (HDACs) inhibitors demonstrated that HDACs are necessary for ZO-2 repression and that HDAC1 is recruited to the E box. We conclude that ZO-2 down-regulates cyclin D1 transcription by interacting with the c-Myc/E box element and by recruiting HDAC1.

show abstract

Section: Discussionmentioning

confidence: 99%

Cyclin D1 Is Transcriptionally Down-Regulated by ZO-2 via an E Box and the Transcription Factor c-Myc

Huerta¹,

Muñoz²,

Tapia

et al. 2007

MBoC

View full text Add to dashboard Cite

show abstract

“…The cast of targets is ponderous (with at least 647 targets already identified, see http://www.myc-cancer-gene.org). Furthermore, recent genomic screens for Myc binding sites by chromatin immunoprecipitation (ChIP) and other genome-wide assays indicate that this number of targets is underestimated by at least half, and (yet another scary thought) these assays have been biased for only those targets having E-boxes in their promoter-regulatory regions (Zeller et al, 2001Fernandez et al, 2003;Haggerty et al, 2003;Mao et al, 2003;O'Connell et al, 2003;Orian et al, 2003).…”

Section: Myc Response Genes In Apoptosis -Moving Targetsmentioning

confidence: 99%

Myc pathways provoking cell suicide and cancer

2003

View full text Add to dashboard Cite

“…Taking advantage of the strong association between CpG islands (CGIs) and gene regulatory regions, 11 one type of microarray constructed with CGIs has been established to identify transcription factor target genes. [12][13][14] To identify genes that are directly regulated by T-bet in Th1 cells, we employed combinatory analysis of chromatin immunoprecipitation (ChIP) and CGI microarray. We described here that ONECUT class of transcriptional activator OC2 is a target gene of T-bet.…”

Section: Introductionmentioning

confidence: 99%

Onecut transcription factor OC2 is a direct target of T-bet in type-1 T-helper cells

et al. 2008

View full text Add to dashboard Cite

T-box transcription factor, T-bet, has a central role in the differentiation of T-helper (Th) progenitor cells to Th1 or Th2 effector cells, partly by regulating the expression of genes such as interferon-g (IFN-g). However, the direct target genes, especially those mediating the transcriptional network initiated by T-bet, are not yet fully understood. By combining chromatin immunoprecipitation from Th1 cells with human cytosine-phosphate-guanine-island array analysis, Onecut 2 (OC2), which encodes a member of the ONECUT class of transcriptional activators, was identified as a direct target gene of T-bet. OC2 is expressed in Th1 but not Th2 cells and reporter assays showed that T-bet transactivates OC2 transcription through putative T-bet half-sites locating À451 to À347 of OC2 promoter region. Moreover, we found that OC2 binds and transactivates human T-bet promoter. These results suggest that not only cell-extrinsic regulation via the IFN-g/STAT1 pathway, but also cell-intrinsic transcriptional positive feedback loop between T-bet and OC2 could be involved in Th1 development.

show abstract

Analysis of Myc Bound Loci Identified by CpG Island Arrays Shows that Max Is Essential for Myc-Dependent Repression

Cited by 165 publications

References 22 publications

Cyclin D1 Is Transcriptionally Down-Regulated by ZO-2 via an E Box and the Transcription Factor c-Myc

Cyclin D1 Is Transcriptionally Down-Regulated by ZO-2 via an E Box and the Transcription Factor c-Myc

Myc pathways provoking cell suicide and cancer

Onecut transcription factor OC2 is a direct target of T-bet in type-1 T-helper cells

Contact Info

Product

Resources

About