Analysis of Mycobacterium avium Complex Serovars Isolated from AIDS Patients from Southeast Brazil

Evaluation of the GenoType Mycobacterium Assay for Identification of Mycobacterial Species from Cultures

“…For instance, M. kansasii sequevar I represents the most common clinical isolate from humans (1,25) and also in our laboratory the majority of the clinical strains identified belong to M. kansasii sequevar I. M. kansasii sequevars III, IV, and V have been isolated much less commonly from humans but have been found in environmental samples (1,12,32). Concerning M. intracellulare subspecies, frequencies of isolated strains differ markedly and M. intracellulare serovar 7 belongs to the rarer isolates found in human specimens (18). Thus, with the CM/AS assay, most of the subspecies were correctly identified through a single banding pattern so that further differentiation with specific probes is not necessary and does not impair the applicability of the test.…”

Isolation and identification of mycobacteria from livestock specimens and milk obtained in Brazil

“…In a national surveillance of mycobacteriosis, from 500 cultures of nontuberculous mycobacteria, M. avium was the major isolates with 44.1% followed by M. kansasii with 13.7% and M. fortuitum with 10.8% (Barreto & Campos 2002). Particularly in immunocompromised patients, the mycobacterial infection caused by M. avium is second only to tuberculosis in prevalence in Brazil (Saad et al 1997). Although it has been established that pasteurization kills M. tuberculosis in milk (Hosty & McDurmont 1975), survival of some nontuberculous Mycobacterium species after simulated laboratory pasteurization has been reported (Harrington & Karison 1965, Grant et al 1996, Stabel et al 1997.…”

Non-tuberculous mycobacteria I: one year clinical isolates identification in Tertiary Hospital Aids Reference Center, Rio de Janeiro, Brazil, in pre highly active antiretroviral therapy era

“…Fandinho et al (1997), in a six months survey, in two hospitals in Rio de Janeiro, showed a rate of 37% of MAC in the blood of Aids patients suspected of disseminated mycobacterial infection. Later, Saad et al (1997), evaluating the dis-tribution of mycobacterial mycosides in clinical isolates from different strain collections, showed that M. avium was the major common opportunistic mycobacterial species isolated from Brazilian Aids patients. In 1999, Conde et al reported 5.8% of NTM identified from inpatients attended at University Hospital (UFRJ) between 1993-94, of which 60% were HIV positive and 27% of the NTM were identified as belonging to the MAC.…”