Analysis of neomycins A, B and C by high-performance liquid chromatography with post-column reaction detection

Electrochemical behavior of neomycin at DNA-modified gold electrodes

“…This method overcomes the limitations of other methods [2,3,[23][24][25][26][27][28][29] in its sensitivity and demand for the DNA samples. The experiments show the modified surfaces exhibit more useful electrochemical properties than unmodified electrode, and so DNA-modified electrodes are used for the quantitative determination of neomycin.…”

“…This method is verified to be more applicable to quantify the binding of the electroactive metal complexes to DNA than those conventional methods [2,3,[23][24][25][26][27][28][29] such as HPLC. On absorption of different amounts of DNA at the electrode, the peak currents begin to decrease and the formal potential shifts to more negative values (see Fig.…”

Development of a liquid chromatographic method for ear drops containing neomycin sulphate, polymyxin B sulphate and dexamethasone sodium phosphate

“…Apffel et al described the analysis of neomycins A-C using reversed-phase ion-pair LC. Detec- tion was performed by post column derivatization of the analytes with ortho-phthaladehyde (OPA) in the presence of mercaptoethanol [11]. Since neomycin has no UV absorbing chromophore, most of the described methods use pre or post column derivatization.…”

Analysis of neomycin using an improved liquid chromatographic method combined with pulsed electrochemical detection

“…Several chromatographic methods combined with different detection techniques have been reported for the determination of neomycin: thin-layer chromatography with detection after derivatization [4,5], LC combined with PED [6][7][8] or evaporative light scattering detection (ELSD) [9,10], pre-column derivatization prior to reversed-phase LC [11][12][13], reversed-phase LC with post-column derivatization [14,15], LC-tandem mass spectrometry using hydrophilic interaction chromatography [16], ion exchange chromatography using refractometric detection [17] and capillary zone electrophoresis with indirect UV detection [18].…”