Analysis of Neural Stem Cells from Krushinskii–Molodkina Rats, Which Have a Genetic Predisposition to Audiogenic Seizures

Saparova, V. B.; Zosen, Denis; Nasluzova, E. V.; Berezovskaya, Anna; Bakhteeva, V. T.; Gorbacheva, E. L.; Chernigovskaya, E. V.; Глазова, М. В.

doi:10.1007/s11055-019-00799-9

Cited by 2 publications

(1 citation statement)

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“…Thus, we revealed the susceptibility of KM NPC to differentiation into excitatory glutamatergic neurons along with the impaired formation of inhibitory GABAergic neurons. These results confirm that recent data were obtained with cultures of NPC isolated from hippocampus of KM rats at the early stage of postnatal development [38]. In particular, it was shown that stimulation by the retinoic acid of KM NPC, but not Wistar NPC, led to predominant differentiation into excitatory glutamate-and dopaminergic neurons [38].…”

Section: Discussionsupporting

confidence: 91%

Glutamatergic Fate of Neural Progenitor Cells of Rats with Inherited Audiogenic Epilepsy

et al. 2020

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Epilepsy is associated with aberrant neurogenesis in the hippocampus and may underlie the development of hereditary epilepsy. In the present study, we analyzed the differentiation fate of neural progenitor cells (NPC), which were isolated from the hippocampus of embryos of Krushinsky-Molodkina (KM) rats genetically prone to audiogenic epilepsy. NPCs from embryos of Wistar rats were used as the control. We found principal differences between Wistar and KM NPC in unstimulated controls: Wistar NPC culture contained both gamma-aminobutyric acid (GABA) and glutamatergic neurons; KM NPC culture was mainly represented by glutamatergic cells. The stimulation of glutamatergic differentiation of Wistar NPC resulted in a significant increase in glutamatergic cell number that was accompanied by the activation of protein kinase A. The stimulation of KM NPC led to a decrease in immature glutamatergic cell number and was associated with the activation of extracellular signal-regulated kinases 1 and 2 (ERK1/2) and protein kinase B/ glycogen synthase kinase 3 beta (Akt/GSK3β), which indicates the activation of glutamatergic cell maturation. These results suggest genetically programmed abnormalities in KM rats that determine the glutamatergic fate of NPC and contribute to the development of audiogenic epilepsy.

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Section: Discussionsupporting

confidence: 91%