Analysis of neurotransmitters in Daphnia magna affected by neuroactive pharmaceuticals using liquid chromatography-high resolution mass spectrometry

Gómez‐Canela, Cristian; García, Xavier Rovira; Martínez‐Jerónimo, Fernando; Marcé, Rosa María; Barata, Carlos

doi:10.1016/j.envpol.2019.113029

Cited by 20 publications

(5 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The main categories of pharmaceuticals are also indicated, showing that neurological drugs (N) are the most consumed, followed by cardiovascular (C), alimentary tract (A), neoplastic (L), respiratory (R) or anti-infective (J), depending on the residence. This is of relevance for neurotoxic compounds as they can have negative effects on biota [ 24 ], antibiotics can be absorbed by plants upon irrigation with river or reclaimed water [ 25 ] or anticancer drugs can have chronic effects on aquatic biota [ 26 ].…”

Section: Resultsmentioning

confidence: 99%

Pharmaceutical Residues in Senior Residences Wastewaters: High Loads, Emerging Risks

2021

Self Cite

View full text Add to dashboard Cite

Senior residences are health-care facilities that are socially-accepted for the assistance of elderly people. Since the elderly account for the foremost pharmaceutical-consuming age-group, senior residences become a hot-spot for pharmaceuticals discharge to the sewage grid. The objectives of the present study were to identify the bioactive pharmaceuticals in sewage waters from senior residences and to propose an on-site monitoring strategy for their control. In this study, we have studied the presence of 43 pharmaceuticals highly consumed by the elderly population in six senior residences located in Spain, France and Portugal. Wastewater was sampled directly from the water-chest in each residence during different times of the day throughout one week. Main compounds detected at the high µg L−1 level were analgesic and antipyretic drugs such as acetylsalicylic acid, paracetamol, ibuprofen; antibiotics such as amoxicillin and sulfamethoxazole; compounds for the treatment of neuropathies as gabapentin, trazodone and valsartan; pharmaceuticals for the treatment of diabetes (vildagliptin) and anticancer drugs. The daily loads discharged were estimated and their fate was evaluated. The final objective of this study is to highlight the need to implement at-source waste water treatment procedures in senior residences, which have been identified as a point source pollution of pharmaceuticals.

show abstract

Section: Resultsmentioning

confidence: 99%

Pharmaceutical Residues in Senior Residences Wastewaters: High Loads, Emerging Risks

2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Finally, the supernatant was filtered using 0.20 μM PTFE filters (DISMIC‐13 JP, Advantec®) and kept in amber chromatographic vials at −80°C until further chromatographic analysis. Analysis of up to 40 neurotransmitters in D. magna whole body extracts was performed by liquid chromatography‐high resolution mass spectrometry following a previously established method (Gómez‐Canela, Rovira, Martínez‐Jerónimo, Marce, & Barata, 2019).…”

Section: Methodsmentioning

confidence: 99%

Reduction of histamine and enhanced spinning behavior of Daphnia magna caused by scarlet mutant

Ismail

Kato

Matsuura

et al. 2020

Genesis

Self Cite

View full text Add to dashboard Cite

The ABC transporter, Scarlet, and its binding partner, White are involved in pigment synthesis in the insect eye and mutations in these genes are used as genetic markers. Recent studies have suggested that these transporters also have additional functions in the neuronal system. In our previous study, we generated scarlet mutant in the small crustacean, Daphnia magna and showed that the mutant lacked the eye pigment in the mutant. Here, we show that the scarlet mutant exhibits spinning behavior. This phenotype is partly associated with the presence of light. Metabolomic analysis of a juvenile mutant revealed that the scarlet mutant has approximately one-tenth of the histamine content of the wild type. Application of histamine to the scarlet mutant rescued the spinning behavior in juveniles, suggesting that the spinning behavior of the mutant is caused by the reduction of histamine. However, the altered behavior was not rescued in the adult mutant by the addition of histamine, suggesting that Scarlet plays an irreversible role in the development of histaminergic neurons. These results suggest that Scarlet plays an important role in histaminergic signaling, which might be related to control the spinning behavior, in addition to its role in eye pigmentation.

show abstract

“…serotonin and norepinephrine reuptake inhibitors) could therefore affect these key plant processes. The cross-species effect of four different neuroactive pharmaceuticals (sertraline, venlafaxine, duloxetine and fluoxetine) has already been shown on the common water flea (D. magna) neurotramsitters (Goḿez-Canela et al, 2019), and the fact that these chemical pathways are conserved in plants suggests the need to better understand neuro-active pharmaceutical interacts in plants too.…”

Section: Neurotransmitters and Neuromotorsmentioning

confidence: 99%

Insights into mode of action mediated responses following pharmaceutical uptake and accumulation in plants

Garduño-Jiménez,

Carter

2024

Front. Agron.

View full text Add to dashboard Cite

The reuse of wastewater to meet irrigation requirements and slurries, sludges and manures as fertilisers to meet crop nutrient demands inadvertently introduces human and veterinary-use pharmaceuticals into the agro-ecosystem. This review synthesises recent research, which has observed sub-lethal effects, following pharmaceutical uptake by plants. Potential links between pharmaceutical mode of action and observed sub-lethal effects in the plant were then examined. Common receptors and biological pathways across species suggests a clear need to integrate plant cellular biology into our understanding of the impacts of pharmaceuticals on important plant functions and processes. To help prioritise future research efforts an analysis of shared mammalian and plant biochemical pathways was undertaken to identify classes of pharmaceuticals which may present a greater risk to key plant functions. These included sulfonylurea antihyperglycemics, steroids, opiods, antipsychotic phenothiazines and pharmaceuticals targeting several neurotransmitters shared between mammals and plants (including beta-blockers, antihistamines and benzodiazepines). Whilst a number of pharmaceutical induced sub-lethal effects have been observed, this review highlights the clear need to study a wider range of pharmaceuticals on a broader range of plant species, including cover crops and wild plants, under realistic exposure scenarios, to fully understand the wider implications of pharmaceutical exposure in agro-ecosystems. State-of-the art omics-techniques offer great potential to understand the mode of action of pharmaceuticals in plants and elucidate links between the pharmaceutical intended mode of action and observed plant effects. In addition, studies under co-stress from pharmaceutical exposure and other stressors such as increased temperatures, drought or pests are lacking and present an urgent research need in the face of feeding a growing population under the threats of climate change.

show abstract

Analysis of neurotransmitters in Daphnia magna affected by neuroactive pharmaceuticals using liquid chromatography-high resolution mass spectrometry

Cited by 20 publications

References 46 publications

Pharmaceutical Residues in Senior Residences Wastewaters: High Loads, Emerging Risks

Pharmaceutical Residues in Senior Residences Wastewaters: High Loads, Emerging Risks

Reduction of histamine and enhanced spinning behavior of Daphnia magna caused by scarlet mutant

Insights into mode of action mediated responses following pharmaceutical uptake and accumulation in plants

Contact Info

Product

Resources

About