Analysis of Neutrophil Proteins of Patients with Behcet's Disease by Two-Dimensional Gel Electrophoresis.

Yamashita, Shinji; Suzuki, Akiko; Yanagita, Tamiko; Hirohata, Shunsei; Kamada, Masafumi; Toyoshima, Satoshi

doi:10.1248/bpb.23.519

Cited by 7 publications

(2 citation statements)

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“…Morphologically, enlarged microtubules [18] and truncated actin filament have been observed in PMNs of BD patients [29]. Functionally, increased chemotactic activity [16,22] as well as production of phagocytic enzymes [1,24,27] and superoxides [30] have been reported.…”

Section: Introductionmentioning

confidence: 99%

Circulating neutrophils in Behçet disease is resistant for apoptotic cell death in the remission phase of uveitis

Fujimori

Ohi

Takeuchi

et al. 2007

Graefes Arch Clin Exp Ophthalmol

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Background Behçet disease (BD) is manifested by recurrent acute iridocyclitis with hypopyon in the active phase, which regresses spontaneously. Hypopyon consists of inflammatory cells infiltrating the eye, with polymorphonuclear cells (PMNs) as the main component. The present study was conducted to investigate the apoptosis property of PMNs in BD patients with uveitis. Methods PMNs were purified from peripheral blood cells of BD patients with uveitis in the active or remission phase and were cultured for 12 hours. In some cultures, lipopolysaccharide (LPS), antagonistic anti-TNFα antibody, agonistic anti-Fas antibody, or Fas:Fc fusion protein was added. At the end of cultures, apoptotic cells were evaluated by Annexin V expression using flow cytometry. Results Spontaneous apoptosis of PMNs showed lower levels in the remission phase of BD-related uveitis compared with the active phase or healthy controls. The lower level of PMN apoptosis in the remission phase of uveitis in BD remained even by stimulation with LPS, anti-TNFα antibody, or Fas:Fc fusion protein, which was abolished in the presence of agonistic anti-Fas antibody. Conclusions In BD patients, the apoptosis of PMNs was reduced in the remission phase of uveitis and restored in the active phase, which arose from the apoptotic cell death in part via Fas-Fas ligand interaction.

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Section: Introductionmentioning

confidence: 99%

Circulating neutrophils in Behçet disease is resistant for apoptotic cell death in the remission phase of uveitis

Fujimori

Ohi

Takeuchi

et al. 2007

Graefes Arch Clin Exp Ophthalmol

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“…It is possible that the inflammatory symptoms are related to the fact that patients with Behçet's disease have hyperfunctional neutrophils. [1][2][3][4] In a previous study, 5) we applied high resolution two-dimensional gel electrophoresis in order to compare the neutrophil proteins of patients with those of healthy volunteers and reported an increase of a truncated actin with an N-terminus of Met-44 in neutrophils of patients with Behçet's disease. Furthermore, we have found that polymorphonuclear (PMN)-elastase specifically cleaves actin at the site between Val-43 and Met-44 in neutrophils of patients with Behçet's disease.…”

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confidence: 99%