Analysis of Nitrites and Nitrates in Hams and Sausages by Open-Tubular Capillary Electrochromatography with a Nanolatex-Coated Capillary Column

Abstract

In this work, a new open-tubular capillary electrochromatography (OT-CEC) method with the nanolatex-coated column was proposed for the determination of nitrites and nitrates in foodstuffs. The method was simple and repeatable as a result of avoiding the introduction of an electroosmotic flow reverse additive (such as cetyltrimethylammonium chloride) in electrophoretic buffer. The limits of quantitation were 0.89 and 1.05 mg kg⁻¹ for nitrate and nitrite, respectively, whereas the overall recoveries ranged from 94 to 103%. The developed OT-CEC method was successfully applied for 12 samples, and the residue profiles of nitrites and nitrates in hams and sausages were obtained and evaluated.

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

“…In general, these additives can be obtained from nature or manufactured by the chemical industry. The main food additives analyzed by CE in the period covered by this review (2013–2015) were sweeteners , preservatives , and colorants .…”

“…Zhan et al. developed an open‐tubular (OT) CEC for the analysis of nitrites and nitrates in hams and sausages . Authors highlighted the high stability of the OT‐CEC system as well as the suitability of the method for its application in the analysis of trace nitrites and nitrates in food samples.…”

Recent advances in microscale separation

“…A number of NPs‐based materials have also been used as stationary phase coatings, including nanolatex , nanochitosan , and magnetic NPs , gold NPs , carbon NPs , silica NPs (see Table ). For example, Gao et al.…”

Open tubular‐capillary electrochromatography: Developments and applications from 2013 to 2015

“…A similar functional group, trimethylamine‐aminated polychloro‐methylstyrene nano‐latex (TMAPL), was also used for bromate analysis . Zhang and coworkers have used TMAPL SP materials to determine nitrites and nitrates in ham and sausages , human urine, and plasma samples . Zhang and coworkers utilized the positively charged free side chain amino groups of prepared nano‐chitosan particles to electrostatically adsorb to negatively charged silanol groups of the inner capillary wall .…”

“…Guo and coworkers have determined the presence of tetracycline antibiotic residues in pig plasma using DMEAPL nanolatex particles , while Lü and coworkers have detected four cephalosporins in milk samples using a chitosan‐based capillary . Qu and coworkers have separated five proteins present in chicken egg whites using a GO‐PDDA nanomaterial , while Zhang and coworkers have determined nitrite and nitrate levels in hams and sausages using TMAPL nanolatex particles . Al‐Hussin and coworkers have separated amino acid enantiomers in orange juice sample using a chiral CD‐PLOT column while Aydoğan and coworkers have determined malic acid enantiomers in apple juice samples using a tentacle‐type polymer functionalized with the chiral selector l ‐histidine .…”