Analysis of noncovalent bonding in Mycoplasma membranes. Kinetics of solubilization in sodium dodecyl sulfate and lithium diiodosalicylate solutions

Choules, G.L.; Sandberg, R. G.; Steggall, Mary; Eyring, Edward M.

doi:10.1021/bi00746a038

Cited by 9 publications

References 16 publications

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The importance of coulombic interactions for the induction of β structure in lysine oligomers by sodium dodecyl sulfate

Mattice

Harrison

1976

Biopolymers

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SynopsisCircular dichroism spectra have been obtained for tri(L-lysine), tetra(L-lysine), and penta(i-lysine) in aqueous sodium dodecyl sulfate a t 25OC. None of the oligomers are affected significantly by sodium dodecyl sulfate a t detergent concentrations exceeding 0.01 M .Literature results show that the high-molecular-weight polymer forms a fi structure under these conditions. M the penta(L-lysine), but not the smaller oligomers, undergoes a conformational change. Its circular dichroism under these conditions is essentially identical to that observed with poly(L-lysine) when i t forms a fi structure in sodium dodecyl sulfate. Solutions of the penta(t,-lysine), which exhibit this modified circular dichroism, are also turbid, leading to the conclusion that the oligomer has formed an intermolecular fi structure. When these experiments are conducted in the presence of 0.1 M sodium hydroxide, the sodium dodecyl sulfate produces neither turbidity nor a modified circular dichroism spectrum. These observations provide compelling evidence that Coulombic interaction between the anionic detergent head and the cationic lysyl amino groups is essential for the conformational change induced in penta(L-lysine) by sodium dodecyl sulfate.A t detergent concentrations near 3.5 X 559:

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