Railway communication is undergoing a transitional period worldwide, and 5G for railway (5G-R), which has been adopted by the Railway International Union (UIC) in China and in many other countries, which is a common concern all over the world. The 2100 MHz frequency band, which ranks among the top three mainstream frequency bands for public 5G network deployment globally, has recently been issued by MIIT for a 5G-R trial in China, and the frequency is fairly close to 5G-R frequency band of 1900 MHz in Europe. Propagation study for railway communication, especially for mainline, which is the most common and widely used scenario, is of great importance, because it is closely related to passengers’ lives and properties. In this article, based on the 5G-R dedicated network testing environment constructed along the test line of loop railway of National Railway Track Test Center of China, the 2100 MHz 5G-R propagation measurement campaign is conducted, with an in-service SS-RSRP signal-based passive 2100 MHz large-scale channel testing system, as well as a SDR-based 2100 MHz 5G-R channel sounding system, based on which the large-scale as well as small-scale channel characteristics are derived and extensively analyzed. With data derived from the passive testing system, the classical FI, CI as well as TR 38.901 large-scale channel models are properly fitted and compared by calculating RMSE as well as MAE values. Moreover, based on the SDR-based channel sounding system, conclusions on the relationship between the parameters of multi-path component numbers, RMS time spread, received power and distance between the transmitter and the receiver are drawn. The 5G-R system studied in this letter is symmetric FDD communication system, apart from the 2 aspects of channel modeling characteristics, which are large-scale and small-scale complementing each other, the research methodology adopted of the letter also has two aspects, passive measurement, as well as active measurement, and both methods have their own advantages and different focuses, which can also complements each other. Relevant research results of this letter will be helpful for facilitating the R&D, deployment, as well as network optimization of a future mobile communication system under railway mainline scenario.