ANALYSIS OF nrLSU GENE TO SUPPORT IDENTIFICATION OF FUNGUS BELONGING TO Cordyceps GENUS AND CLAVICIPITACEAE FAMILY

Abstract

Nucleotide sequences of the nuclear large ribosomal subunit (nrLSU) have been used in fungal systematics for a long time. nrLSU was also used in Cordyceps and related genera within the Clavicipitaceae family. A previously identified sample by morphology and ITS was used in this research to analyze the ability of nrLSU to support the identification of entomopathogenic fungi. Our results show that we successfully amplified nrLSU gene using the primer pair LR0R and LR5. The PCR product on agarose gel showed a clear band at 950 bp. Sequencing method was then adopted and proofread before molecular phylogenetic analysis was applied with reference sequences obtained from the publication of Sung et al. Once again, this analysis confirms the DL0004 specimen as Cordyceps neovolkiana.

Observations of the host habitat of Ophiocordyceps nutans in the Bidoup Nui Ba National Park, Lam Dong, Vietnam.

“…Results of Vu Tien Luyen et al shows that, using the primer pair LR0R and LR5 to analyze the nrLSU gene to support identification of fungus belonging to Cordyceps genus and Clavicipitaceae family. The final analysis confirms the DL0004 specimen as Cordyceps neovolkiana [22].…”