Analysis of nucleus activity in malpighian tubules of Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae) larvae by critical electrolyte concentration

Fermino, Fábio; Conte, Hélio; Falco, José Ricardo Penteado

doi:10.1590/s1519-566x2010000400016

Cited by 6 publications

(3 citation statements)

References 14 publications

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“…Fermino et al (2010) also used CECs to study the nuclear activity of Malpighian tubules of D. saccharalis, which are divided into 3 regions: proximal, middle, and distal; differences in the CECs between the regions were identified. The 5th instar has chromatin that is more decondensed than in the fourth instar.…”

Section: Discussionmentioning

confidence: 99%

Critical electrolyte concentration of silk gland chromatin of the sugarcane borer Diatraea saccharalis, induced using agrochemicals

Gigliolli¹,

Fermino²,

Moreira³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. The sugarcane borer Diatraea saccharalis is widely known as the main pest of sugarcane crop, causing increased damage to the entire fields. Measures to control this pest involve the use of chemicals and biological control with Cotesia flavipes wasps. In this study, we evaluated the insecticides fipronil (Frontline; 0.0025%), malathion (Malatol Bio Carb; 0.4%), cipermetrina (Galgotrin; 10%), and neem oil (Natuneem; 100%) and the herbicide nicosulfuron (Sanson 40 SC; 100%) in the posterior region silk glands of 3rd-and 5th-instar D. saccharalis by studying the variation in the critical electrolyte concentration (CEC). Observations of 3rd-instar larvae indicated that malathion, cipermetrina, and neem oil induced increased chromatin condensation that may consequently disable genes. Tests with fipronil showed no alteration in chromatin condensation. With the use of nicosulfuron, there was chromatin and probable gene decompaction. In the 5th-instar larvae, the larval CEC values indicated that malathion and neem oil induced increased chromatin condensation. The CEC values for 5th-instar larvae using cipermetrina, fipronil, and nicosulfuron Agrochemical-induced CEC in sugarcane borer indicated chromatin unpacking. These observations led us to conclude that the quantity of the pesticide does not affect the mortality of these pests, can change the conformation of complexes of DNA, RNA, and protein from the posterior region of silk gland cells of D. saccharalis, activating or repressing the expression of genes related to the defense mechanism of the insect and contributing to the selection and survival of resistant individuals.

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Section: Discussionmentioning

confidence: 99%

Critical electrolyte concentration of silk gland chromatin of the sugarcane borer Diatraea saccharalis, induced using agrochemicals

Gigliolli¹,

Fermino²,

Moreira³

et al. 2014

Genet. Mol. Res.

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“…However, the maintenance of the integrity of the host organs is paramount for the life cycle of the parasitoid [41] [42]. In D. saccharalis parasitized by C. flavipes, no morphological alterations were detected in the Malpighian tubules and the ampullae, except for the presence of hemocytes and teratocytes.…”

Section: Discussionmentioning

confidence: 99%

Malpighian Tubules in Larvae of Diatraea saccharalis (Lepidoptera; Crambidae): A Morphological Comparison between Non-Parasitized and Parasitized by Cotesia flavipes (Hymenoptera; Braconidae)

Rigoni¹,

Conte²

2014

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In Diatraea saccharalis larvae, the Malpighian tubules are found along the digestive tube, extending from the middle mesenteric region to the end of the posterior intestine, where they come in contact with the rectum to form the cryptonephridium. Scanning and transmission electron microscopy of non-parasitized and parasitized larvae by Cotesia flavipes have indicated that the tubules consist of secretory and reabsorption cells. In parasitized larvae, the occurrence of hemocytes and teratocytes around the tubules is indicative of their role in immunological defense; however, they were not observed in non-parasitized larvae. At day 9 of parasitism, the mitochondria-containing vacuoles and myelin-like figures show signs of degeneration. The results of this study have confirmed that C. flavipes manipulates the physiology and biochemistry of D. saccharalis because the Malpighian tubules of the parasitized larvae remain active until the parasitoid completes its pupal stage and is released from the host organism.

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“…In B. mori, as in other Lepidoptera, the MT comprises six tubules, three on each side of the body of the insect, which unite to form a sac-like structure known as the ampullae in the region of the pyloric valve, the site of the junction of middle and posterior intestine (TEIGLER; ARNOTT, 1972;LEVY et al, 2004LEVY et al, , 2008MARANHÃO, 1978;RIGONI et al, 2004). The tubules can be further subdivided into three regions from the ampullae: the proximal, which extends to the middle portion of the midgut; the medial, with tubules that return to the pyloric valve; and the distal, with tubules that continue to the rectum, which makes up the cryptonephric system (FERMINO et al, 2010;RIGONI et al, 2004). Functionally, the proximal and medial sections act in the absorption of ions and the transport of fluids, forming urine (CHAPMAN, 1998;GULLAN;CRANSTON, 2010).…”

Section: Introductionmentioning

confidence: 99%

Ampullae infection of the malpighian tubules of Bombyx mori by Alphabaculovirus

Santorum¹,

Beu²,

Ribeiro³

et al. 2017

Biosci. J.

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ABSTRACT:Alphabaculovirus is a genus of the entomopathogenic virus, whose species Bombyx mori nucleopolyhedrovirus (BmNPV) infects the silkworm, Bombyx mori, which is an important insect in the sericulture industry. A geographic isolate of BmNPV was identified in the state of Paraná, Brazil. It was infecting B. mori larvae and various organs and target tissues were identified, however, there was no information about the infection of Malpighian tubules (MT). The MT comprises the excretory system of B. mori and acts in the elimination of toxic substances and in hydroelectrolytic homeostasis. Thus, the present study examined the susceptibility and cytopathology of B. mori MT to BmNPV. To this end, hybrid fifth instar larvae were inoculated with a virus suspension at different days post-inoculation (dpi). MT segments were collected and divided into the ampullae, proximal, medial and distal regions. These were processed for light microscopy and transmission electron analysis. The MT regions revealed differences in susceptibility to BmNPV and the ampullae in its transition area was infected from the sixth dpi; the other regions did not reveal any evidence of infection. The transition area of the ampullae has not been previously described in Lepidoptera and its cytopathology revealed a hypertrophic nucleus with viroplasm, followed by the formation and development of viral polyhedra, which are common characteristics of infections by Alphabaculovirus. Thus, infection of the ampullae of the MT of B. mori by BmNPV, together with other known targets, compromises the metabolic balance of the insect, which results in consequences for silk production and damage to the sericulture sector.

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Analysis of nucleus activity in malpighian tubules of Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae) larvae by critical electrolyte concentration

Cited by 6 publications

References 14 publications

Critical electrolyte concentration of silk gland chromatin of the sugarcane borer Diatraea saccharalis, induced using agrochemicals

Critical electrolyte concentration of silk gland chromatin of the sugarcane borer Diatraea saccharalis, induced using agrochemicals

Malpighian Tubules in Larvae of Diatraea saccharalis (Lepidoptera; Crambidae): A Morphological Comparison between Non-Parasitized and Parasitized by Cotesia flavipes (Hymenoptera; Braconidae)

Ampullae infection of the malpighian tubules of Bombyx mori by Alphabaculovirus

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