Analysis of oil synthesis pathway in Cyperus esculentus tubers and identification of oleosin and caleosin genes

Zhu, Youcheng; Wang, Ying; Wei, Zunmiao; Zhang, Xiaokai; Jiao, Bingyang; Tian, Yu; Yang, Fan; Li, Jingwen; Liu, Yajing; Yang, Xuguang; Zhang, Jinhao; Wang, Xinyue; Mu, Zhongsheng; Wang, Qingyu

doi:10.1016/j.jplph.2023.153961

Cited by 4 publications

(4 citation statements)

References 49 publications

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“…The results of our study also showed a significant decrease in the pH level and a significant increase in the SWC of soil when C. esculentus was intercropped with legumes. These results are supported by previous studies, such as that of Wang et al. (2023) , who observed a considerable decrease in soil pH after intercropping tea with legumes compared with monocropping.…”

Section: Discussionsupporting

confidence: 91%

“…It is widely distributed in many northern temperate regions and is becoming increasingly popular as an energy crop in China, India, Egypt, and the United States ( Ayeni, 2022 ). C. esculentus possesses exceptional traits, such as drought resistance and salinity tolerance ( Duan et al., 2022 ; Zhang et al., 2023 ; Zhu et al., 2023 ). The tubers of C. esculentus are rich in fats, proteins, sugars, other nutrients, and a wide range of active compounds, making it a high-quality, high-yield, and high-value crop ( Coskuner et al., 2002 ).…”

Section: Introductionmentioning

confidence: 99%

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Effect of intercropping with legumes at different rates on the yield and soil physicochemical properties of Cyperus esculentus L. in arid land

Shen,

Liu,

et al. 2024

Front. Plant Sci.

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Intercropping has the potential to enhance yields and nutrient availability in resource-limited agricultural systems. However, the effects on crop yield nutrients and soil properties can vary considerably depending on the specific plant combinations and intercropping ratios used. In this study, the advantages and impacts of intercropping C. esculentus with legumes were investigated by measuring their biomass, nutrient content, and soil properties. The experiment included five intercropping treatments: monoculture of C. esculentus (MC), intercropping of C. esculentus with Medicago sativa L. (alfalfa) at row spacing ratios of 4:4 (4:4CM) and 8:4 (8:4CM), and intercropping of C. esculentus with Glycine max (L.) Merr. (soybean), also at row spacing ratios of 4:4 (4:4CG) and 8:4 (8:4CG). Our results demonstrated that all four intercropping treatments (4:4CM, 4:4CG, 8:4CM, and 8:4CG) significantly increased the biomass of C. esculentus by approximately 41.05%, 41.73%, 16.08%, and 18.43%, respectively, compared with monoculture cultivation alone, among which the 4:4CG treatment was optimum. However, no significant differences were observed in alfalfa or soybean biomass across different intercropping ratios. A notable increase was found in the total nitrogen (TN) and total phosphorus (TP) contents in the leaves, roots, and tubers of C. esculentus under intercropping, along with increased soil organic carbon (SOC), alkaline-hydrolyzed nitrogen (AN), available phosphorus (AP), microbial biomass carbon (MBC), microbial biomass nitrogen (MBN), and soil water content (SWC), and significantly reduced the soil pH. Among the intercropping treatments, the 4:4CG treatment also exhibited the most favorable soil properties. In particular, compared with MC, the 4:4CG treatment resulted in significant increases of 163.8%, 394.6%, and 716.8% in SOC, AN, and AP contents, respectively. The same treatment also led to significant increases of 48.34%, 46.40%, and 208.65% in MBC, MBN, and SWC, respectively. Overall, the findings suggest that the use of 4:4CG intercropping is an effective approach for sustainable farming management in Xinjiang.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Effect of intercropping with legumes at different rates on the yield and soil physicochemical properties of Cyperus esculentus L. in arid land

Shen,

Liu,

et al. 2024

Front. Plant Sci.

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“…To data, oleosin protein have been successively reported in different oil crops, such as soybean [ 53 ], vernicia tree [ 54 ] and peanut [ 55 ] etc. In Cyperus esculentus , 9 OLE and 21 CLO genes were identified, which can be provided a reference for the development of strategies to improve oil content of C. esculentus tubers [ 56 ]. In Carthamus tinctorius , 8 putative OLE genes were identified from the genome database, providing a way of elucidating the intricate mechanisms of oil body synthesis [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of key genes for triacylglycerol biosynthesis and storage in herbaceous peony (Paeonia lactifolra Pall.) seeds based on full-length transcriptome

Xu,

Li,

et al. 2024

BMC Genomics

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Background The herbaceous peony (Paeonia lactiflora Pall.) is extensively cultivated in China due to its root being used as a traditional Chinese medicine known as ‘Radix Paeoniae Alba’. In recent years, it has been discovered that its seeds incorporate abundant unsaturated fatty acids, thereby presenting a potential new oilseed plant. Surprisingly, little is known about the full-length transcriptome sequencing of Paeonia lactiflora, limiting research into its gene function and molecular mechanisms. Results A total of 484,931 Reads of Inserts (ROI) sequences and 1,455,771 full-Length non-chimeric reads (FLNC) sequences were obtained for CDS prediction, TF analysis, SSR analysis and lncRNA identification. In addition, gene function annotation and gene structure analysis were performed. A total of 4905 transcripts were related to lipid metabolism biosynthesis pathway, belonging to 28 enzymes. We use these data to identify 10 oleosin (OLE) and 5 diacylglycerol acyltransferase (DGAT) gene members after de-redundancy. The analysis of physicochemical properties and secondary structure showed them similarity in gene family respectively. The phylogenetic analysis showed that the distribution of OLE and DGAT family members was roughly the same as that of Arabidopsis. Quantitative real-time polymerase chain reaction (qRT–PCR) analyses revealed expression changes in different seed development stages, and showed a trend of increasing and then decreasing. Conclusion In summary, these results provide new insights into the molecular mechanism of triacylglycerol (TAG) biosynthesis and storage during the seedling stage in Paeonia lactiflora. It provides theoretical references for selecting and breeding oil varieties and understanding the functions of oil storage as well as lipid synthesis related genes in Paeonia lactiflora.

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“…Given the essential role of OLEs in the biosynthesis and degradation of TAGs, many OLE genes have been identified and characterized in various plant species, including safflower (Carthamus tinctorius), rice (Oryza sativa), flax (Linum usitatissimum), castor bean (Ricinus communis), Arabidopsis (Arabidopsis thaliana), tiger nut (Cyperus esculentus), and rapeseed (Brassica napus) (Chen et al 2019;Hyun et al 2013;Kim et al 2002;Liu et al 2012;Lu et al 2018;Zhu et al 2023). This indicates that the OLE family is highly abundant and diverse in land plants.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification of oleosin family and expression analysis in response to abiotic stresses in balloon flower (Platycodon grandiflorus)

Kim,

Hyun

2024

J Plant Biotechnol

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Oleosins (OLEs) are structural proteins commonly found within oil bodies (OBs), playing a significant role in regulating the number, size, and stability of OBs. Therefore, this study aims to comprehensively analyze the OLE family in balloon flower (Platycodon grandiflorus) -a significant medicinal plant. Through genome-wide exploration and bioinformatics analyses, we identified and characterized five putative OLE proteins from P. grandiflorus (PlgOLEs). This study provides a comprehensive overview of this gene family in balloon flowers, including phylogenic analysis, conserved motifs, isoelectric points, and hydrophobicity. The study revealed the presence of central hydrophobic regions with a proline knot motif, a characteristic feature shared by OLE proteins in various plant species. Additionally, tissue-specific expression analysis revealed that PlgOLEs were predominantly expressed in seeds, indicating their crucial role in seed OB stability. Furthermore, expression profiling under abiotic stresses demonstrated that PlgOLEs are stress-inducible genes, suggesting their diverse physiological functions in stress responses. These findings shed light on the functional diversity of OLEs in balloon flowers and establish a basis for further research, including genetic modification studies, to elucidate their physiological roles.

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Analysis of oil synthesis pathway in Cyperus esculentus tubers and identification of oleosin and caleosin genes

Cited by 4 publications

References 49 publications

Effect of intercropping with legumes at different rates on the yield and soil physicochemical properties of Cyperus esculentus L. in arid land

Effect of intercropping with legumes at different rates on the yield and soil physicochemical properties of Cyperus esculentus L. in arid land

Identification of key genes for triacylglycerol biosynthesis and storage in herbaceous peony (Paeonia lactifolra Pall.) seeds based on full-length transcriptome

Genome-wide identification of oleosin family and expression analysis in response to abiotic stresses in balloon flower (Platycodon grandiflorus)

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