Analysis of oral microorganism diversity in healthy individuals before and after chewing areca nuts using PCR-denatured gradient gel electrophoresis

Xiong, Xiong; Hou, Aixiang; Yi, Shuhan; Guo, Yichen; Zhao, Ziwei; Wu, Zhongkun; Cheng, Huan; Li, Ke; Li, Zongjun; Ren, Youhua; Wang, Yuanliang

doi:10.1016/j.aninu.2018.07.001

Cited by 6 publications

(3 citation statements)

References 11 publications

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“…The abundance of Serratia (p-value = 0.022), Veillonella (p-value = 0.015), Streptococcus (p-value = 0.026) and Leptotrichia (p-value = 0.022) bacterial genera was found to be significantly associated with gutka chewers (Figure 3b). Our results are in agreement with a previous study which by using denaturing gradient gel electrophoresis (DGGE) correlated the abundance of Streptococcus and Veillonella in response to usage of betel nut preparations 31 . Serratia species are gram-negative, facultatively anaerobic bacteria possessing the ability to act as opportunistic pathogens in immunocompromised patients.…”

Section: Discussionsupporting

confidence: 93%

Microbiome evaluation revealed salivary dysbiosis in addicts of betel nut preparations

Saleem

Mujtaba

Kori

et al. 2020

Preprint

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Betel nut addiction is recognized as the causative agent of oral microbiome dysbiosis and other systematic disorders. A number of betel nut preparations containing ingredients such as slaked lime, catechu extract and tobacco are being commonly used particularly in South Asia. The underlying variations in the oral microbiome due to usage of betel nut preparations are poorly understood. We evaluated salivary microbiome in response to chewing of betel nut preparation(s). In order to assess the microbiome dynamics, metagenomic analysis of 16S rRNA gene (V3-V4 hypervariable region) from salivary bacteria in chewers of betel nut preparation (n = 16) and non-chewers (n = 55) was carried out by Greengenes and SILVA ribosomal sequence databases. It was observed that Gutka chewers demonstrated lower alpha diversity and number of bacterial genera than the non-chewers. Taxonomic assignment on phylum level revealed Firmicutes (p-value = 0.042 at 95% confidence interval) to be significantly more abundant in Gutka chewers in comparison with non-chewers. Beta diversity analysis at genus level by weighted unifrac distance matrices unveiled both groups to be divergent from each other. On the genus level, Veillonella (p-value = 0.015), Streptococcus (p-value = 0.026), Leptotrichia (p-value = 0.022) and Serratia (p-value = 0.022) species appeared to be significantly more abundant in Gutka chewers in comparison to non-chewers. The present study suggests salivary dysbiosis in response to gutka chewing and concludes that gutka chewers possess higher abundance of acidogenic and aciduric bacteria. This study contributes additional information regarding oral microbiome variations with response to gutka consumption.

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Section: Discussionsupporting

confidence: 93%

Microbiome evaluation revealed salivary dysbiosis in addicts of betel nut preparations

Saleem

Mujtaba

Kori

et al. 2020

Preprint

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“…As a matter of fact, areca nut is one of the four major southern medicines in China [ 10 ] and is recorded in the Compendium of Materia Medica [ 11 ]. Since 1953, areca nut has been included in the Pharmacopoeia of China [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Bioactive Components of Areca Nut: An Overview of Their Positive Impacts Targeting Different Organs

Sun,

Yu,

et al. 2024

Nutrients

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Areca catechu L. is a widely cultivated tropical crop in Southeast Asia, and its fruit, areca nut, has been consumed as a traditional Chinese medicinal material for more than 10,000 years, although it has recently attracted widespread attention due to potential hazards. Areca nut holds a significant position in traditional medicine in many areas and ranks first among the four southern medicines in China. Numerous bioactive compounds have been identified in areca nuts, including alkaloids, polyphenols, polysaccharides, and fatty acids, which exhibit diverse bioactive functions, such as anti-bacterial, deworming, anti-viral, anti-oxidant, anti-inflammatory, and anti-tumor effects. Furthermore, they also display beneficial impacts targeting the nervous, digestive, and endocrine systems. This review summarizes the pharmacological functions and underlying mechanisms of the bioactive ingredients in areca nut. This helps to ascertain the beneficial components of areca nut, discover its medicinal potential, and guide the utilization of the areca nut.

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“…Several techniques can be used to determine whether malaria parasite DNA is present following PCR amplification [11]. Gel electrophoresis is a popular technique that uses an electric field to separate the amplified DNA fragments according to size [12]. DNA-staining dyes can be used to see the resultant bands.…”

Section: Introductionmentioning

confidence: 99%

Designing Microfluidic PCR Chip Device Using CFD Software for the Detection of Malaria

Austria,

Garcia,

Caparanga

et al. 2023

Computation

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Polymerase chain reaction (PCR) technique is one of the molecular methods in amplifying DNA for the detection of malaria. However, the collection and transportation of samples and the processing and dissemination of results via conventional PCR, especially when used for routine clinical practice, can hamper the technique’s sensitivity and specificity. The rampancy of such disease in the Philippines is aggravated by the limited supply of medical machinery and the poor economic state of the country; thus, the need to innovate a device for the early detection of malaria is necessary. With that, this study focuses on designing a microfluidic device that will mimic the function of a conventional genus-specific PCR based on the 18S rRNA gene to detect malaria parasites (Plasmodium falciparum) at low-grade parasitemia. The design was intended to be portable, accessible, and economical, which none from past literature has dealt with specifically for malaria detection. This in silico design is a first in the country specially crafted for such reasons. The proposed device was developed and simulated using ANSYS software for Computational Fluid Dynamics (CFD) analyses. The simulation shows that adding loops to the design increases its relative deviation but minimally compared to having only a straight path design. This indicates that looping is acceptable in designing a microfluidic device to minimize chip length. It was also found that increasing the cross-sectional area of the fluid path decreases the efficiency of the design. Lastly, among the three materials utilized, the chip made of polypropylene is the most efficient, with a relative deviation of 0.94 compared to polycarbonate and polydimethylsiloxane, which have relative deviations of 2.78 and 1.92, respectively. Future researchers may mesh the 44-cycle microfluidic chip due to the limitations of the software used in this study, and other materials, such as biocomposites, may be assessed to broaden the application of the design.

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Analysis of oral microorganism diversity in healthy individuals before and after chewing areca nuts using PCR-denatured gradient gel electrophoresis

Cited by 6 publications

References 11 publications

Microbiome evaluation revealed salivary dysbiosis in addicts of betel nut preparations

Microbiome evaluation revealed salivary dysbiosis in addicts of betel nut preparations

Bioactive Components of Areca Nut: An Overview of Their Positive Impacts Targeting Different Organs

Designing Microfluidic PCR Chip Device Using CFD Software for the Detection of Malaria

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