Analysis of organic and non-organic arsenious or selenious compounds by capillary electrophoresis

Albert, M.; Demesmay, Claire; Rocca, J. L.

doi:10.1007/bf00322913

Cited by 68 publications

(39 citation statements)

References 18 publications

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“…Preliminary experiments used 2% acetic acid (pH 2) as the CE electrolyte aiming for protonation of Se species. The pK values of Se species were higher than two (organic Se species) or close to two (inorganic Se species) [18]. The applied voltage was set to +18 kV.…”

Section: Methods Development 'Offline'mentioning

confidence: 99%

“…As a first approach a method was developed for getting a separation of the six species in one capillary zone electrophoresis (CZE) run. Partly, the upper pK values of the Se species are considerably high, e.g., Se (IV): 8.3, SeM: 9.21 [18]; therefore, an electrolyte/pH was chosen which deprotonized all Se species of interest. Thus, the species were analyzed in the anionic form, principally moving to the anode and in opposite direction to a fast EOF.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, carrier-free CE affords an advantage compared with liquid chromatography [16]. Several articles deal with application of CE to Se speciation problems [12,18,191. However, analytical solutions are not available for all Se species.…”

Section: Introductionmentioning

confidence: 99%

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Selenium speciation by interfacing capillary electrophoresis with inductively coupled plasma‐mass spectrometry

Michalke

Schramel

1998

Electrophoresis

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The high resolution potential of capillary electrophoresis (CE) makes CE techniques valuable for separations of selenium species. Further, inductively coupled plasma-mass spectrometry (ICP-MS) affords element-specific multi-element detection, providing extremely low detection limits. The combination of CE with ICP-MS promises to become a powerful tool for metal speciation. Therefore, an on-line hyphenation of CE with ICP-MS, which was developed earlier (Michalke, B., Schramel, P., Fresenius' J. Anal. Chem. 1997, 257, 594-599), was modified and applied to selenium speciation. For this reason, capillary zone electrophoresis (CZE) methods were developed, providing the possibility to analyze six Se species of interest in one run: Se (IV), Se (VI), selenium carrying glutathione (GSSeSG), selenomethionine (SeM), selenocystine (SeC), and selenocystamine (SeCM). The final CE method used an alkaline background electrolyte (Na2CO3/NaOH) with separation differentiated from the detection step during hyphenation. This resulted in short separation times (10 min) and a subsequent detection step of 100s. The Se species were sufficiently separated from each other and appeared at 7s (SeCM), 16s (Se [VI]), 22s [SeC], 27s (Se [IV]), 35s [SeM] and 56s (GSSeSG) during the detection step. Detection limits were calculated as 10 or 20 microg Se/L for inorganic Se species and 35-50 microg Se/L for organic Se species.

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Section: Methods Development 'Offline'mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Selenium speciation by interfacing capillary electrophoresis with inductively coupled plasma‐mass spectrometry

Michalke

Schramel

1998

Electrophoresis

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“…Various instrumental techniques including capillary electrophoresis (CE), high performance liquid chromatography -inductively coupled plasma -mass spectrometry (HPLC-ICP-MS), and ion chromatography -inductively coupled plasma -mass spectrometry (IC-ICP-MS) have been used by others for the speciation of selenium/tellurium-containing compounds (Casiot et al, 2002;Vogt and Werner, 1994;Schlegel et al, 1996;Albert et al, 1995;Gilon and Potin-Gautier, 1996;Kannamkumarath et al, 2002;Kahakachchi et al, 2004;Caruso and B'Hymer, 2006).…”

Section: Analytical Methods For Detection Of Compounds Of Interestmentioning

confidence: 99%

Capillary electrophoretic determination of selenocyanate and selenium and tellurium oxyanions in bacterial cultures

Pathem

Pradenas

Castro

et al. 2007

Analytical Biochemistry

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Experiments were also designed to detect the biological production of selenocyanate in Se-amended bacterial cultures using IC-ICP-MS. A selenium-resistant bacterium, 130404, when amended with 1.0 mM selenate biologically produced selenocyanate in replicate cultures grown to stationary phase. The amounts produced were statistically significantly different than the amounts of SeCN -produced in sterile but warmed growth media. The headspace gases above growing cultures with or without metalloid amendments were analyzed using gas chromatography. Organo-selenium iii compounds that were detected were methaneselenol, dimethyl selenide, dimethyl selenenyl sulfide, dimethyl diselenenyl sulfide, dimethyl diselenide. Two late eluting peaks were observed in the same cultures that were not characterized but can reasonably be inferred to be dimethyl diselenenyl sulfide and dimethyl triselenide.

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“…[49][50][51][52] This method led to low detection limits, usually in the mg l -1 range with a small sample volume of only a few nanoliters. Nevertheless, the lack of selectivity and sensitivity of the UV detector is a limit to its application to more specific and sensitive complex matrices.…”

Section: Selenoamino Acidsmentioning

confidence: 99%

Speciation of Selenium Compounds

Pyrzyńska

1998

ANAL. SCI.

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Analysis of organic and non-organic arsenious or selenious compounds by capillary electrophoresis

Cited by 68 publications

References 18 publications

Selenium speciation by interfacing capillary electrophoresis with inductively coupled plasma‐mass spectrometry

Selenium speciation by interfacing capillary electrophoresis with inductively coupled plasma‐mass spectrometry

Capillary electrophoretic determination of selenocyanate and selenium and tellurium oxyanions in bacterial cultures

Speciation of Selenium Compounds

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