Analysis of Oxidative Stress Enzymes and Structural and Functional Proteins on Human Aortic Tissue from Different Aortopathies

Soto, Marí­a Elena; Soria-Castro, Elizabeth; Guarner-Lans, Verónica; Ontiveros, Eleazar Muruato; Mejía, Benjamín Iván Hernández; Hernandez, Humberto Jorge Martínez; García, Rodolfo Barragán; Herrera, Valentín; Pérez-Torres, Israel

doi:10.1155/2014/760694

Cited by 47 publications

(67 citation statements)

References 88 publications

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“…Needless to say, it remains possible that apocynin has other beneficial off‐target affects, including acting as a ROS‐scavenger, blocking other elastases or increasing SMC proliferation . Several investigators have studied the involvement of ROS in thoracic aortic root/ascending aortic aneurysm formation, including individuals with MFS, as well as patients with bicuspid aortic valves . The hypothesized important role of ROS in MFS aneurysm formation presented here agrees with the findings of Jimenez‐Altayo et al who also reported increased nitrotyrosine residues in both aortic aneurysms and cultured SMC from MFS patients .…”

Section: Discussionsupporting

confidence: 87%

“…C, In vitro quantification of NADPH oxidase activity by LGCL in Fbn1 AS and Des SMCs treated with AngII or vehicle (n = 6, *P = .03). D, In vitro quantification of NADPH oxidase activity by LGCL in Fbn1 AS SMCs treated with AngII and with either TGF-β neutralizing antibody (TGFb NA) or vehicle (n = 6, *P = .009) ascending aortic aneurysm formation, including individuals with MFS, 24,27,[40][41][42] as well as patients with bicuspid aortic valves. [43][44][45] The hypothesized important role of ROS in MFS aneurysm formation presented here agrees with the findings of Jimenez-Altayo et al who also reported increased nitrotyrosine residues in both aortic aneurysms and cultured SMC from MFS patients.…”

Section: Discussionmentioning

confidence: 99%

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Anatomically specific reactive oxygen species production participates in Marfan syndrome aneurysm formation

Emrich

Penov

Arakawa

et al. 2019

J Cellular Molecular Medi

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Marfan syndrome (MFS) is a connective tissue disorder that results in aortic root aneurysm formation. Reactive oxygen species (ROS) seem to play a role in aortic wall remodelling in MFS, although the mechanism remains unknown. MFS Fbn1C1039G/+ mouse root/ascending (AS) and descending (DES) aortic samples were examined using DHE staining, lucigenin‐enhanced chemiluminescence (LGCL), Verhoeff's elastin‐Van Gieson staining (elastin breakdown) and in situ zymography for protease activity. Fbn1C1039G/+ AS‐ or DES‐derived smooth muscle cells (SMC) were treated with anti‐TGF‐β antibody, angiotensin II (AngII), anti‐TGF‐β antibody + AngII, or isotype control. ROS were detected during early aneurysm formation in the Fbn1C1039G/+ AS aorta, but absent in normal‐sized DES aorta. Fbn1C1039G/+ mice treated with the unspecific NADPH oxidase inhibitor, apocynin reduced AS aneurysm formation, with attenuated elastin fragmentation. In situ zymography revealed apocynin treatment decreased protease activity. In vitro SMC studies showed Fbn1C1039G/+‐derived AS SMC had increased NADPH activity compared to DES‐derived SMC. AS SMC NADPH activity increased with AngII treatment and appeared TGF‐β dependent. In conclusion, ROS play a role in MFS aneurysm development and correspond anatomically with aneurysmal aortic segments. ROS inhibition via apocynin treatment attenuates MFS aneurysm progression. AngII enhances ROS production in MFS AS SMCs and is likely TGF‐β dependent.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Anatomically specific reactive oxygen species production participates in Marfan syndrome aneurysm formation

Emrich

Penov

Arakawa

et al. 2019

J Cellular Molecular Medi

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“…In addition, other studies in animal models of MFS have shown that the expressions of the precursor enzymes of O 2 − , xanthine oxidase, and NADPH oxidase are increased [ 8 ]. A previous study from our laboratory has shown an increase in oxidative stress and an alteration of the antioxidant enzymes super oxide dismutase (SOD) and glutathione peroxidase (GPx) in the aortic aneurysm of MFS patients [ 9 ]. In addition, antioxidants enzymes such as glutathione peroxidase (GPx) and glutathione-S-transferase (GST) require GSH as cofactor for ROS detoxification [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Infusion ofHibiscus sabdariffa L.Modulates Oxidative Stress in Patients with Marfan Syndrome

Soto

Zúñiga-Muñoz

Guarner-Lans

et al. 2016

Mediators of Inflammation

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Marfan syndrome (MFS) is associated with progressive aortic dilatation, endothelial dysfunction, and oxidative stress that contribute to the early acute dissection of the vessel and can end up in rupture of the aorta and sudden death. Many studies have described that the organic acids from Hibiscus sabdariffa Linne (HSL) calyces increase cellular antioxidant capacity and decrease oxidative stress. Here we evaluate if the antioxidant properties of HSL infusion improve oxidative stress in MFS patients. Activities of extra cellular super oxide dismutase (ECSOD), glutathione peroxidase (GPx), glutathione-S-transferase (GST), glutathione reductase (GSSG-R), glutathione (GSH), lipid peroxidation (LPO) index, total antioxidant capacity (TAC), and ascorbic acid were determined in plasma from MFS patients. Values before and after 3 months of the treatment with 2% HSL infusion were compared in control and MFS subjects. After treatment, there was a significant decrease in ECSOD (p = 0.03), EGPx (p = 0.04), GST (p = 0.03), GSH (p = 0.01), and TAC and ascorbic acid (p = 0.02) but GSSG-R activity (p = 0.04) and LPO (p = 0.02) were increased in MFS patients in comparison to patients receiving the HSL treatment and C subjects. Therefore, the infusion of HSL calyces has antioxidant properties that allow an increase in antioxidant capacity of both the enzymatic and nonenzymatic systems, in the plasma of the MSF patients.

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“…Endothelial dysfunction and downregulated NO would contribute to the stiffness, reduced distensibility, and aortic complications such as AOD [ 5 ] . Our results showed a decrease of nitrite levels in SAS group may be due to endothelium dysfunction.…”

Section: Discussionmentioning

confidence: 99%

Oxidative Damage in Human Aorta of Patients with Advanced Aortoiliac Occlusive Disease

Lucas

Carraro

Belló‐Klein

et al. 2016

Brazilian Journal of Cardiovascular Surgery

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IntroductionOxidative stress seems to be a role in the atherosclerosis process, but research in human beings is scarce.ObjectiveTo evaluate the role of oxidative stress on human aortas of patients submitted to surgical treatment for advanced aortoiliac occlusive disease.MethodsTwenty-six patients were divided into three groups: control group (n=10) formed by cadaveric organ donors; severe aortoiliac stenosis group (patients with severe aortoiliac stenosis; n=9); and total aortoiliac occlusion group (patients with chronic total aortoiliac occlusion; n=7). We evaluated the reactive oxygen species concentration, nicotinamide adenine dinucleotide phosphate-oxidase, superoxide dismutase and catalase activities as well as nitrite levels in samples of aortas harvested during aortofemoral bypass for treatment of advanced aortoiliac occlusive disease.ResultsWe observed a higher level of reactive oxygen species in total aortoiliac occlusion group (48.3±9.56 pmol/mg protein) when compared to severe aortoiliac stenosis (33.5±7.4 pmol/mg protein) and control (4.91±0.8 pmol/mg protein) groups (P<0.05). Nicotinamide adenine dinucleotide phosphate oxidase activity was also higher in total aortoiliac occlusion group when compared to the control group (3.81±1.7 versus 1.05±0.31 µmol/min.mg protein; P<0.05). Furthermore, superoxide dismutase and catalase activities were significantly higher in the severe aortoiliac stenosis and total aortoiliac occlusion groups when compared to the control cases (P<0.05). Nitrite concentration was smaller in the severe aortoiliac stenosis group in comparing to the other groups.ConclusionOur results indicated an increase of reactive oxygen species levels and nicotinamide adenine dinucleotide phosphate-oxidase activity in human aortic samples of patients with advanced aortoiliac occlusive disease. The increase of antioxidant enzymes activities may be due to a compensative phenomenon to reactive oxygen species production mediated by nicotinamide adenine dinucleotide phosphate oxidase. This preliminary study offers us a more comprehensive knowledge about the role of oxidative stress in advanced aortoiliac occlusive disease in human beings.

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Analysis of Oxidative Stress Enzymes and Structural and Functional Proteins on Human Aortic Tissue from Different Aortopathies

Cited by 47 publications

References 88 publications

Anatomically specific reactive oxygen species production participates in Marfan syndrome aneurysm formation

Anatomically specific reactive oxygen species production participates in Marfan syndrome aneurysm formation

Infusion ofHibiscus sabdariffa L.Modulates Oxidative Stress in Patients with Marfan Syndrome

Oxidative Damage in Human Aorta of Patients with Advanced Aortoiliac Occlusive Disease

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