Analysis of oxidized glycerophosphocholine lipids using electrospray ionization mass spectrometry and microderivatization techniques

Harrison, Kathleen A.; Davies, Sean S.; Marathe, Gopal K.; McIntyre, Thomas M.; Prescott, Stephen M.; Reddy, K. Mohan; Falck, J. R.; Murphy, Robert Cushman

doi:10.1002/(sici)1096-9888(200002)35:2<224::aid-jms933>3.3.co;2-7

Cited by 15 publications

(18 citation statements)

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“…2). MALDI‐TOF/TOF tandem MS analysis of fragmentation of the OxPC positive ions indicates a phosphotidylcholine head group but does not provide information about the position of oxidation or chain length of the fatty acid (data not shown); however, a broad collection of studies on OxPCs strongly suggests the same series of masses as oxidation species when analyzed by ESI or MALDI . Additionally, the highly abundant positive species PC(34:0) at m/z 734.5 exhibited less decrease relative to the global average (Supplemental Fig.…”

Section: Resultsmentioning

confidence: 99%

Monitoring time‐dependent degradation of phospholipids in sectioned tissues by MALDI imaging mass spectrometry

2014

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Imaging mass spectrometry (IMS) is useful for visualizing the localization of phospholipids on biological tissue surfaces creating great opportunities for IMS in lipidomic investigations. With advancements in IMS of lipids, there is a demand for large-scale tissue studies necessitating stable, efficient and well-defined sample handling procedures. Our work within this article shows the effects of different storage conditions on the phospholipid composition of sectioned tissues from mouse organs. We have taken serial sections from mouse brain, kidney and liver thaw mounted unto ITO-coated glass slides and stored them under various conditions later analyzing them at fixed time points. A global decrease in phospholipid signal intensity is shown to occur and to be a function of time and temperature. Contrary to the global decrease, oxidized phospholipid and lysophospholipid species are found to increase within 2 h and 24 h, respectively, when mounted sections are kept at ambient room conditions. Imaging experiments reveal that degradation products increase globally across the tissue. Degradation is shown to be inhibited by cold temperatures, with sample integrity maintained up to a week after storage in -80 °C freezer under N2 atmosphere. Overall, the results demonstrate a timeline of the effects of lipid degradation specific to sectioned tissues and provide several lipid species which can serve as markers of degradation. Importantly, the timeline demonstrates oxidative sample degradation begins appearing within the normal timescale of IMS sample preparation of lipids (i.e. 1-2 h) and that long-term degradation is global. Taken together, these results strengthen the notion that standardized procedures are required for phospholipid IMS of large sample sets, or in studies where many serial sections are prepared together but analyzed over time such as in 3-D IMS reconstruction experiments.

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Section: Resultsmentioning

confidence: 99%

Monitoring time‐dependent degradation of phospholipids in sectioned tissues by MALDI imaging mass spectrometry

2014

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“…PC has pro‐ and anti‐inflammatory activities with a variety of oxidative modifications to polyunsaturated sn‐2 fatty acyl substituents 34 . Some of these lipid species are implicated in the formation of oxLDL 35 and atherosclerotic lesions 36 . PC molecules include a lot of acyl chains that differ in length and double bond positions.…”

Section: Discussionmentioning

confidence: 99%

Insights into the prognosis of lipidomic dysregulation for death risk in patients with coronary artery disease

Qin

Zhu

Lai

et al. 2020

Clinical & Translational Med

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Background: Dyslipidaemia contributes to the progression of coronary artery disease (CAD) toward adverse outcomes. Plasma lipidomic measure may improve the prognostic performances of clinical endpoints of CAD. Our research is designed to identify the correlations between plasma lipid species and the risks of death, major adverse cardiovascular event (MACE) and left ventricular (LV) remodeling in patients with CAD. Methods: A total of 1569 Chinese patients with CAD, 1011 single-centre patients as internal training cohort, and 558 multicentre patients as external validation cohort, were enrolled. The concentration of plasma lipids in both cohorts was determined through widely targeted lipidomic profiling. Least absolute shrinkage and selection operator Cox and multivariate Cox regressions were used to develop prognostic models for death and MACE, respectively. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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“…Reverse-phase HPLC coupled with thermospray ionization has been used to identify lipid hydroperoxides and hydroperoxyl fatty acids esterified to phospholipids. Murphy et al applied electrospray ionization (ESI) MS to the direct analysis of lipid hydroperoxides and other oxidation products (25,39,49). ESI in the negative ion mode allowed the generation of abundant carboxylate anions from free acid hydroperoxides.…”

Section: Ag + Cis-msmentioning

confidence: 99%

New Insights Regarding the Autoxidation of Polyunsaturated Fatty Acids

Yin

Porter

2005

Antioxidants & Redox Signaling

166

106

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Free radical-initiated autoxidation of polyunsaturated fatty acids (PUFAs) has been implicated in numerous human diseases, including atherosclerosis and cancer. This review covers the free radical mechanisms of lipid oxidation and recent developments of analytical techniques to analyze the lipid oxidation products. Autoxidation of PUFAs generates hydroperoxides as primary oxidation products, and further oxidation leads to cyclic peroxides as secondary oxidation products. Characterization of these oxidation products is accomplished by several mass spectrometric techniques. Ag+ coordination ion spray mass spectrometry has proven to be a powerful tool to analyze the intact lipid peroxides. Monocyclic peroxides, bicyclic endoperoxides, serial cyclic peroxides, and a novel class of endoperoxides (dioxolane-isoprostane peroxides) have been identified from the oxidation of arachidonate. Electron capture atmospheric pressure chemical ionization mass spectrometry has been applied to study lipid oxidation products after derivatization. All eight possible diastereomeric isoprostanes are observed from the oxidation of a single hydroperoxide precursor. 5- and 15-series isoprostanes are more abundant than the 8- and 12-series because the precursors that lead to 8- and 12-series compounds can undergo further oxidation and form dioxolane-isoprostane peroxides. Furthermore, formation of isoprostanes from 15-hydroperoxyeicosatetraenoate occurs from beta-fragmentation of the corresponding peroxyl radical to generate a pentadienyl radical rather than a "dioxetane" intermediate, as previously suggested.

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Analysis of oxidized glycerophosphocholine lipids using electrospray ionization mass spectrometry and microderivatization techniques

Cited by 15 publications

References 0 publications

Monitoring time‐dependent degradation of phospholipids in sectioned tissues by MALDI imaging mass spectrometry

Monitoring time‐dependent degradation of phospholipids in sectioned tissues by MALDI imaging mass spectrometry

Insights into the prognosis of lipidomic dysregulation for death risk in patients with coronary artery disease

New Insights Regarding the Autoxidation of Polyunsaturated Fatty Acids

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