Analysis of P67 Expression in Different Mouse Tissues

Abstract

A ~1.9kb cDNA containing the entire coding region, 5' UTR, and 3' UTR is cloned from rat tumor hepatoma (KRC-7) cells. It was further characterized for protein expression in rabbit reticulocyte lysates. Later, this cDNA was used for extensive mutagenesis and biochemical studies to identify specific domains/sites of rat p67 that are essential for its functions. To investigate whether similar size transcript is also present in mouse tissues, we examined the tissue distributions of mouse p67 transcript and protein from male and female mice. We found that there are two mouse p67 transcriptsone is ~1.4kb long whereas the other is ~2.2 kb long. The ~1.4 kb long transcript is present in almost all tissues tested whereas the ~2.2 kb transcript is present in brain, spleen, skeletal muscle, and possibly in lungs. Our analysis for the tissue distributions of p67 protein detected several proteins corresponding to ~67 kDa, ~60 kDa, 52 kDa, 26 kDa, and possibly others in different proportions in various tissues of both male and female mice. Keywords: mouse p67 gene, tissue distribution of mouse p67 transcript, tissue distribution of p67 in both male and female mice Abbreviations used kb, kilobase pairs; p67, a 67 kDa glycoprotein that binds to both eukaryotic initiation factor 2 (eIF2) and extracellular signal-regulated kinases 1 & 2 (ERK1/2); p52, the downstream 108-480 segment of p67; and p26, the N-terminal 1-107 amino acid segment of p67.