Vehicle automation is among the best possible solutions for traffic issues, including traffic accidents, traffic jams, and energy consumption. However, the user acceptance of automated vehicles is critical and is affected by riding comfort. In addition, human factors in automated vehicle control should be clear. This study evaluates the effect of different courses on driving comfort in automated vehicles using field experiments with 25 subjects. This study focused on lateral motion, but speed control was not targeted. Further, generating a path for obstacle avoidance and lane keeping, which have several constraining conditions, was also not targeted. Rendering a comfortable path is beneficial for developing an acceptable system as a car developer and for building new curves for automated or driving assistance systems from the perspective of construction. The automated vehicle drove at a speed of 30 km/h on four courses, namely, clothoid, two types of spline curves, and arc, based on the real intersection. Each participant sat on both the driver and passenger seat and answered a questionnaire. The experimental data indicated the clothoid course to be the most comfortable, while the arc was most uncomfortable for a significance level of 1%. These tendencies are applicable to driver and passenger seats, all genders, and experiences and will be beneficial for human factor research in automated vehicle control.