Analysis of Pathophysiology of Pressure Ulcer in a Microcirculatory Model

Abstract

Aim: Chronic osteomyelitis with pus discharge is very difficult to treat. This time we experienced the case of lower leg osteomyelitis, which we have treated with Trafermin spray. The result was excellent and beyond our expectation, so we will report this case.Case: 63 years, male. He sustained the open fracture injury of his right foot by a fallen rocks and resulted in the osteomyelitis. Then he was introduced to our hospital. Operation was done under spinal anesthesia, the infected bone and covering skin were excised fully and the defect (4 · 3 · 3 cm) was left open. After 2 weeks of dressing change, we began to use the Trafermin spray.Result: At the first 3 weeks, evident change was not recognized, but good granulation formation started 4 weeks later. This tendency has continued for a while and the defect completely closed 5 months later. Discussion:We have experienced the case of lower leg osteomyelitis, which showed the dramatic improvement. Through this case, we have reconfirmed that rich blood supply around the defect is essential for the healing process when we use the Trafermin spray.Aim: Taking advantage of homogeneously marked cells from green fluorescent protein (GFP) transgenic mice, we have previously demonstrated that bone marrow derived stromal cells (BSCs) differentiate into a variety of cell lineages both in vitro and in vivo. In the present study, we extend this approach to characterize adipose tissue derived stromal cells (ASCs), sometimes called processed lipoaspirate (PLA) cells.Methods: ASCs were isolated from inguinal fat pads of GFP mice. After the primary culture in control medium, the cells were incubated in osteogenic and chondrogenic medium for 2 to 4 weeks, respectively. Osteogenic and chondrogenic differentiation were assessed by the special staining and RT-PCR.Results: ASCs incubated in osteogenic medium were stained positively for von Kossa and alkaline phosphatase staining. RT-PCR analysis showed the Expression of osteocyte related genes. Positive staining cells for alcian blue and the expression of chodrocyte related genes were found in ASCs incubated in chondrogenic medium.Conclusions: These findings suggest that ASCs derived from GFP transgenic mice have both osteochondrogenic potential in vitro. Since this cell population can be easily identified through fluorescence microscope, it may be an ideal source of ASCs for further experiments of stem cell biology and tissue engineering.Aim: In the present study, expression of members of the fibroblast growth factor (FGF) family and early growth response gene 1 (Egr-1), known as a transcription factor, was analyzed by reverse transcription polymerase chain reaction (RT-PCR).Methods: After the mouse fetal small intestine was divided into single cells with collagenase-disperse, the cells were cultured. After reorganized small intestine were resected with a knife, expression of mRNAs from FGF family and Egr-1 was analyzed by RT-PCR.Results: The wound surface was covered with epithelial cells by 24 hours after resection.Among members of ...