Analysis of pharmaceuticals and other solutes of biochemical importance by supercritical fluid chromatography

Abstract

SummaryThe feasibility of using supercritical fluid chromatography (SFC) for analysis of polar and/or ionic analytes of interest to the pharmaceutical industry is described. Specifically, the analyses of cyclosporin (a cyclic undecapeptide), several ionophores, and a group of fat-soluble vitamins are illustrated. The separation of a group of fat-soluble vitamins is evaluated on two bonded stationary phases, DB-5 (95'/0 dimethyl-5%-diphenylpolysiloxane) and DB-WAX (Carbowax 20M type, "bonded"). Lastly, a new restrictor technology known as a converging-diverging restrictor is described.

Determination of vitamins D2 and D3 in pharmaceuticals by supercritical-fluid extraction and HPLC separation with UV detection

“…Vitamin D in pharmaceutical products has been determined by gas chromatography [4], supercritical-fluid chromatography [5], and, most frequently, by liquid chromatography [6][7][8][9]. These methods involve liquidliquid or solid -liquid extraction for sample preparation, and are wasteful of organic solvents and time as a result.…”

Fluidos supercríticos em química analítica. II. Cromatografia com fluido supercrítico: instrumentação

“…Bertsch e Green 17 compararam 5 tipos de restritores: restritor de sílica fundida, restritor de sílica fundida não tratada, restritor de aço inox, restritor de vidro Pyrex e restritor de filtro sinterizado microporoso, tendo obtido um melhor resultado com o primeiro. A Figura 3 ilustra, de forma esquemática, diferentes geometrias de restritores que foram investigados para SFC e SFC-MS [18][19][20][21][22][23] .…”

Applications of Supercritical Fluids in Industrial Analysis

“…With a molecular weight of 1202 it is at first sight an unpromising candidate for capillary SFC with unmodified C0 2 • However, White et al (1988) obtained good peak shape using a 9 m x 50 ftm i.d. fused-silica capillary column coated with a 0.20 ftm film of DB-5.…”

“…The capillary SFC of some polar ionophores used as veterinary antibiotics and a range of fat-soluble vitamins (including vitamins A, A acetate, K1, E, E acetate and K3, and provitamins D, D2 and D3) with C0 2 as mobile phase was reported by White et al (1988). In addition to the SFC-MS of mitomycin C described earlier, Musser and Callery (1990) also demonstrated the microbore packed-column SFC-MS of three further anticancer drugs ( diaziquone, thiotepa and cyclophosphamide) under the same conditions.…”