Analysis of phenolic pollutants in human samples by high performance capillary electrophoresis based on pretreatment of ultrasound-assisted emulsification microextraction and solidification of floating organic droplet

Wang, Huili; Yan, Hao; Wang, Chengjun; Chen, Fan; Ma, Meiping; Wang, Wenwei; Wang, Xuedong

doi:10.1016/j.chroma.2012.06.088

Cited by 39 publications

(12 citation statements)

References 31 publications

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“…In particular, the LODs of CSAC-UASEM-HPLC were in the range of 0.02-0.10 mg=L, which are much lower than those for solid phase microextraction-HPLC (1.1-2.4 mg=L) (Nerin et al 2002), ionic liquid cold-induced aggregation-DLLME-HPLC (0.58 mg=L), and molecular complex-DLLME (29 mg=L) (Hu et al 2010). On the other hand, the LODs of CSAC-UASEM-HPLC were comparable to those of UAEM-sodification of floating organic drop combined with HPCE (0.01-0.04 mg=L) in urine and blood samples (H. L. Wang et al 2012). The comparable RSDs (3.7-8.1%) in Arthrospira (Spirulina) platensis were obtained with the reported sample preparation techniques, as listed in Table 5.…”

Section: Microextraction Of Phenolic Pollutants 1255supporting

confidence: 55%

“…The comparable RSDs (3.7-8.1%) in Arthrospira (Spirulina) platensis were obtained with the reported sample preparation techniques, as listed in Table 5. The previously reported techniques focused on the preparation of water or urine samples, while this proposed technique may be used in preparation of Arthrospira (Spirulina) platensis samples, which are more complicated matrices than waters (Saraji and Marzban 2010) and urine samples (H. L. Wang et al 2012). Most importantly, CSAC-UASEM does not require environmentally harmful dispersive solvents required in DLLME procedures.…”

Section: Microextraction Of Phenolic Pollutants 1255mentioning

confidence: 97%

“…Chromatogram of five phenolics in water and Arthrospira (Spirulina) platensis samples by CSAC-UASEM technique. Conditions: extraction solvent, CHCl 3 (50 mL); surfactant, sodium dodecyl sulfate (500 mL); sample solution temperature, 25 AE 1 C; pH, 3; ultrasonic extraction time, 3 min; no salt addition; without fortification of phenolics or fortification at 1.0 mg=L phenolics; 800 W ultrasonic power, at on:off cycle of 5 s:3 s, and cycle times of 30. higher enrichment factors (201-269) than molecular complex-DLLME (55) (Hu et al 2010), ionic liquid cold-induced aggregation-DLLME (112) (Jiang et al 2011), and UAEM-solidification of floating organic drop (114-172) (H. L. Wang et al 2012). In particular, the LODs of CSAC-UASEM-HPLC were in the range of 0.02-0.10 mg=L, which are much lower than those for solid phase microextraction-HPLC (1.1-2.4 mg=L) (Nerin et al 2002), ionic liquid cold-induced aggregation-DLLME-HPLC (0.58 mg=L), and molecular complex-DLLME (29 mg=L) (Hu et al 2010).…”

Section: Microextraction Of Phenolic Pollutants 1255mentioning

confidence: 99%

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Determination of Phenolics in Water andArthrospira(Spirulina)platensisby Concentrated Sulfuric Acid and Ultrasound-Assisted Surfactant-Enhanced Emulsification Microextraction and High Performance Liquid Chromatography

Wang

et al. 2014

Analytical Letters

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A novel, practical, and environmentally friendly technique, termed concentrated sulfuric acid cleanup and ultrasound-assisted surfactant-enhanced emulsification microextraction (CSAC-UASEM), was combined with HPLC for the preconcentration and determination of five phenolics in water and Arthrospira (Spirulina) platensis samples. The main advantages include that the concentrated sulfuric acid is used to decrease macromolecular interferences prior to microextraction and, unlike dispersive liquid-liquid microextraction procedures, no dispersive organic solvent is required. Chloroform and sodium dodecyl sulfate were used as the extraction solvent and emulsifier, respectively. The algal cell preparation and CSAC-UASEM procedure parameters, including selection of cleanup method, ultrasound power, cell cytocylasis time, type and volume of extraction solvent, extraction temperature, ultrasound-extracted time, and sample pH, were optimized. At the fortification levels of 1.0 and 10.0 lg/L, the enrichment factors of analytes were in the range of 201.38 to 269.24. The percent extraction ranged from 71.57% to 107.42% in environmental Arthrospira-350, -793, and -834 samples, whereas the range was from 74.17% to 106.72% in water samples. The limits of detection (at S=N ¼ 3) were 0.02 to 0.04 lg/L (except for 4-bromobisphenol A of 0.10 lg/L). These values indicate an approximately ten-fold improvement compared with the values reported by other techniques. In summary, the CSAC-UASEM sample preparation technique has great potential in the routine determination of trace phenolics in environmental waters and aquatic biological samples.

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Section: Microextraction Of Phenolic Pollutants 1255supporting

confidence: 55%

Section: Microextraction Of Phenolic Pollutants 1255mentioning

confidence: 97%

Section: Microextraction Of Phenolic Pollutants 1255mentioning

confidence: 99%

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Determination of Phenolics in Water andArthrospira(Spirulina)platensisby Concentrated Sulfuric Acid and Ultrasound-Assisted Surfactant-Enhanced Emulsification Microextraction and High Performance Liquid Chromatography

Wang

et al. 2014

Analytical Letters

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“…Nowadays, there is growing interest in the health threat posed by endocrine disrupting chemicals (EDCs) and phenolic pollutants . These pollutants originate mostly from the domestic waste water (from the use of detergents and personal care products and pharmaceuticals) and leachates from the agriculture area or from the industrial waste water that eventually flow into environmental water bodies such as rivers, lake, and sea .…”

Section: Introductionmentioning

confidence: 99%

Online preconcentration by electrokinetic supercharging for separation of endocrine disrupting chemical and phenolic pollutants in water samples

et al. 2016

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Online preconcentration using electrokinetic supercharging (EKS) was proposed to enhance the sensitivity of separation for endocrine disrupting chemical (methylparaben (MP)) and phenolic pollutants (2-nitrophenol (NP) and 4-chlorophenol (CP)) in water sample. Important EKS and separation conditions such as the concentration of BGE; the choice of terminating electrolyte (TE); and the injection time of leading electrolyte (LE), sample, and TE were optimized. The optimum EKS-CE conditions were as follows: BGE comprising of 12 mM sodium tetraborate pH 10.1, 100 mM sodium chloride as LE hydrodynamically injected at 50 mbar for 30 s, electrokinetic injection (EKI) of sample at -3 kV for 200 s, and 100 mM CHES as TE hydrodynamically injected at 50 mbar for 40 s. The separation was conducted at negative polarity mode and UV detection at 214 nm. Under these conditions, the sensitivity of analytes was enhanced from 100- to 737-fold as compared to normal CZE with hydrodynamic injection, giving LOD of 4.89, 5.29, and 53 μg/L for MP, NP and CP, respectively. The LODs were adequate for the analysis of NP and CP in environmental water sample having concentration at or lower than their maximum admissible concentration limit (240 and 2000 μg/L for NP and CP). The LOD of MP can be suitable for the analysis of MP exists at mid-microgram per liter level, even though the LOD was slightly higher than the concentration usually found in water samples (from ng/L to 1 μg/L). The method repeatabilities (%RSD) were in the range of 1.07-2.39% (migration time) and 8.28-14.0% (peak area).

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“…When salts were added in a non-saturated state, some researchers found that salt additions to the aqueous sample had differential effects on microextraction: it may enhance, not influence, or limit extraction (Wu, Tragas, Lord, & Pawliszyn, 2002). In these studies, the NaCl dissolved in the aqueous solution may have changed the physical properties of the Nernst diffusion film and reduced the rate of diffusion of the target analyte into the microdrop (Psillakis & Kalogerakis, 2001), thus affecting the extraction efficiency except for salting-out effect (Wang et al, 2012). In our study, the addition of 2.5 g of Na 2 SO 4 was selected as the optimum salt amount for subsequent experiments.…”

Section: Selection Of Solvent and Saltmentioning

confidence: 99%

Optimization of a phase separation based magnetic-stirring salt-induced liquid–liquid microextraction method for determination of fluoroquinolones in food

Gao

Wang

et al. 2015

Food Chemistry

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a b s t r a c tHerein, we developed a novel integrated apparatus to perform phase separation based on magneticstirring, salt-induced, liquid-liquid microextraction for determination of five fluoroquinolones in animal-based foods by HPLC analysis. The novel integrated apparatus consisted of three simple HDPE (high density polyethylene) parts that were used to separate the solvent from the aqueous solution prior to retrieving the extractant. The extraction parameters were optimized using the response surface method based on central composite design: 791 lL of acetone solvent, 2.5 g of Na 2 SO 4 , pH 1.7, 3.0 min of stir time, and 5.5 min centrifugation. The limits of detection were 0.07-0.53 lg kg À1 and recoveries were 91.6-105.0% for the five fluoroquinolones from milk, eggs and honey. This method is easily constructed from inexpensive materials, extraction efficiency is high, and the approach is compatible with HPLC analysis. Thus, it has excellent prospects for sample pre-treatment and analysis of fluoroquinolones in animal-based foods.

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Analysis of phenolic pollutants in human samples by high performance capillary electrophoresis based on pretreatment of ultrasound-assisted emulsification microextraction and solidification of floating organic droplet

Cited by 39 publications

References 31 publications

Determination of Phenolics in Water andArthrospira(Spirulina)platensisby Concentrated Sulfuric Acid and Ultrasound-Assisted Surfactant-Enhanced Emulsification Microextraction and High Performance Liquid Chromatography

Determination of Phenolics in Water andArthrospira(Spirulina)platensisby Concentrated Sulfuric Acid and Ultrasound-Assisted Surfactant-Enhanced Emulsification Microextraction and High Performance Liquid Chromatography

Online preconcentration by electrokinetic supercharging for separation of endocrine disrupting chemical and phenolic pollutants in water samples

Optimization of a phase separation based magnetic-stirring salt-induced liquid–liquid microextraction method for determination of fluoroquinolones in food

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