Analysis of phenols in water by high-performance liquid chromatography using coumarin-6-sulfonyl chloride as a fluorogenic precolumn label

Suliman, Fakhr Eldin O.; Al-Kindi, Suad S.; Al-Kindy, Salma M.Z.; Lawati, Haider A. J. Al

doi:10.1016/j.chroma.2005.09.094

Cited by 57 publications

(25 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, many phenols are not strong acids and therefore show low ionization efficiency in ESI-MS, leading to higher detection limits compared with the limits that are obtained for many highly ionizable compounds. Efforts have been focused on chemical derivatization as a method for enhancing the detection of phenolic compounds by HPLC with fluorescence [13] and mass spectrometric detection [14]. For example, derivatization of phenols with dansyl chloride was originally developed to incorporate a fluorogenic label into the analytes to improve fluorescence detection in HPLC [15,16].…”

Section: Introductionmentioning

confidence: 99%

1,2-Dimethylimidazole-4-sulfonyl chloride, a novel derivatization reagent for the analysis of phenolic compounds by liquid chromatography electrospray tandem mass spectrometry: Application to 1-hydroxypyrene in human urine

Spink

2007

Journal of Chromatography B

View full text Add to dashboard Cite

A novel derivatization method employing 1,2-dimethylimidazole-4-sulfonyl chloride (DMISC) to improve the mass spectrometric response for phenolic compounds in liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS) and tandem mass spectrometry (LC-ESI-MS/MS) is described. Several environmentally relevant compounds, including chloro-, aryl-and alkylphenols, steroidal estrogens, and hydroxy-polycyclic aromatic hydrocarbons (OHPAHs), were selected to evaluate this technique. A facile derivatization procedure employing DMISC results in dimethylimidazolesulfonyl (DMIS) derivatives that are stable in aqueous solution. These DMIS derivatives produced intense [M+H] + ions in positive-ion LC-ESI-MS. The product ion spectra of the [M+H] + ions of simple phenols were dominated by ions representing the DMIS and dimethylimidazole moieties, whereas product ion spectra of the DMIS derivatives of OHPAHs with three or more fused aromatic rings showed prominent ArO + ions, the relative intensity of which increased with the number of rings. The DMIS derivatives of the selected phenolic compounds showed excellent chromatographic properties. To substantiate the utility of derivatization with DMISC, an analytical method employing enzyme hydrolysis, solid phase extraction, derivatization with DMISC, and analysis by LC-ESI-MS/MS with multiple reaction monitoring for determination in human urine of 1-hydroxypyrene, a widely used biomarker for the assessment of human exposure to PAHs, was developed and validated.

show abstract

Section: Introductionmentioning

confidence: 99%

1,2-Dimethylimidazole-4-sulfonyl chloride, a novel derivatization reagent for the analysis of phenolic compounds by liquid chromatography electrospray tandem mass spectrometry: Application to 1-hydroxypyrene in human urine

Spink

2007

Journal of Chromatography B

View full text Add to dashboard Cite

show abstract

“…The solvent concentration ranges (SCR) were MeOH (40 -60%), ACN (30 -40%), and THF (30 -35%). The retention factors, k, were obtained from the retention times of PPs and from the retention time of a solution of KNO 3 . When retention factors (ln k) for PPs were plotted versus the organic modifier concentration, b, linear plots were obtained and selectivity changes were observed.…”

Section: Separation Characteristics Of Pps Using An Hyp Columnmentioning

confidence: 99%

“…Nitrophenols are used as pesticides, insecticides, herbicides, and synthetic intermediates and are produced photochemically in the atmosphere from exhaust gases. Chlorophenols have a different origin and they are used as fungicides, herbicides, wood preservatives, and in water disinfection process [3,4]. Some of these compounds are either known or suspected endocrine disruptors or carcinogens [5,6].…”

Section: Introductionmentioning

confidence: 99%

Comparison of the performance of different reversed‐phase columns for liquid chromatography separation of 11 pollutant phenols

Castro

Santos-Montes

Izquierdo-Hornillos³

et al. 2007

J of Separation Science

View full text Add to dashboard Cite

A systematic optimization of the HPLC separation of a mixture containing 11 pollutant phenols (PPs) using a Hypersil ODS (250 mm64.6 mm, 5 lm) column and UV-DAD detection has been carried out. The binary mobile phases used were obtained by mixing 50 mM phosphate (pH = 3.0) and methanol, ACN, or THF as organic modifiers. After selecting ACN as an organic modifier, the effects of pH and temperature on PPs separation were studied. A mobile phase of 50 mM acetate (pH = 5.0) -ACN (60 : 40 v/v) at 508C allowed the separation of 11 phenols but not to baseline in 17 min. To improve the performance of this separation, the following RP columns were tested: Luna C18 (2), Purospher C18, Synergi C12, Synergi Fusion C18, Gemini C18, Luna Cyano, Lichrospher C8, and Envirosep-PP (polymeric). In all the cases, the performance (analysis time, retention, selectivity, resolution, asymmetry factors, and efficiency) was evaluated. A further reoptimization of the mobile phase was carried out for all the columns by studying the ACN content and pH, with the aim of improving the above-mentioned separations and selecting the most suitable one for PPs analysis.

show abstract

“…They are usually needed to derive with suitable derivatization reagent to increase their volatility before injection into GC. HPLC coupled with various detectors are used for the determination of phenols, such as ultraviolet (UV) [8][9][10], fluorescence [11] and mass spectroscopy (MS) [12]. To improve the sensitivity of CE, online/inline stacking techniques have been presented, such as large volume sample stacking [13].…”

Section: Introductionmentioning

confidence: 99%

Molecularly imprinted polymers for dispersive solid‐phase extraction of phenolic compounds in aqueous samples coupled with capillary electrophoresis

et al. 2016

View full text Add to dashboard Cite

A simple and sensitive method for the simultaneous determination of seven phenolic compounds in aqueous samples was developed by dispersive solid-phase extraction (DSPE) coupled with capillary electrophoresis (CE), namely phenol, 4-chlorophenol (4-CP), pentachlorophenol (PCP), 2,4,6-trichlorophenol (2,4,6-TCP), 2,4-dichlorophenol (2,4-DCP), 2-chlorophenol (2-CP) and 2,6-dichlorophenol (2,6-DCP). Molecularly imprinted polymers (MIPs) prepared by precipitation polymerization with 2,4,6-trichlorophenol (2,4,6-TCP) as template were used as DSPE sorbents to selectively adsorb the phenols. Various parameters affecting the extraction efficiency were evaluated and excellent CE separation was achieved within 13.5 min by using 15 mmol/L borate buffer containing 15% (v/v) methanol at pH = 9.8. Under the optimized conditions, good linearity was obtained in the range of 2-200 g/L for phenol, 2,4-DCP and 2,6-DCP, 2-300 g/L for 4-CP and 2-CP, 1.5-150 g/L for PCP, and 2-400 g/L for 2,4,6-TCP, with the correlation coefficient (R 2 ) higher than 0.9938. Limits of detection and limits of quantification were in the range of 0.18-0.44 g/L and 0.59-1.45 g/L, respectively. The developed MIPs-DSPE-CE method was also successfully applied to determine the seven phenolic compounds in lake, tap, seawater and tannery wastewater. The average recoveries at three different spiked levels ranged from 70.75 to 106.7% with the relative standard deviations of 1.15-6.28%.

show abstract

Analysis of phenols in water by high-performance liquid chromatography using coumarin-6-sulfonyl chloride as a fluorogenic precolumn label

Cited by 57 publications

References 28 publications

1,2-Dimethylimidazole-4-sulfonyl chloride, a novel derivatization reagent for the analysis of phenolic compounds by liquid chromatography electrospray tandem mass spectrometry: Application to 1-hydroxypyrene in human urine

1,2-Dimethylimidazole-4-sulfonyl chloride, a novel derivatization reagent for the analysis of phenolic compounds by liquid chromatography electrospray tandem mass spectrometry: Application to 1-hydroxypyrene in human urine

Comparison of the performance of different reversed‐phase columns for liquid chromatography separation of 11 pollutant phenols

Molecularly imprinted polymers for dispersive solid‐phase extraction of phenolic compounds in aqueous samples coupled with capillary electrophoresis

Contact Info

Product

Resources

About