Analysis of phospholipids, sterols, and fatty acids in Taxus chinensis var. mairei cells in response to shear stress

Abstract

Plant cell culture has been developed as an alternative method for the production of an anticancer drug, paclitaxel. However, the sensitivity of plant cells to shear stress has been one of the main obstacles to the scale-up of the plant cell culture. To gain a better understanding of the mechanism of plant cells' response to hydrodynamic mechanical stress, lipid profiling of suspension-cultured Taxus chinensis (Chinese yew) var. mairei cells under shear stress was carried out using liquid chromatography-tandem MS and gas chromatography-time-of-flight MS. T. chinensis var. mairei cells cultured in a Couette-type shear reactor responded with an increase of cell membrane permeability compared with control cells, which indicated that the adaptation to shear stress altered membrane lipid composition. The main changes of lipid profiles in the shear-stress-induced cells were the following: (a) the total phospholipid content decreased, especially that of structural phospholipids such as phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine; (b) cells showed increased stigmasterol at the expense of sitosterol and campesterol; (c) the stigmasterol/phospholipid ratio increased; (d) the mono-unsaturated-fatty-acid content increased; (e) the shear-stress-induced cells accumulated very-long-chain saturated fatty acids (C22:0, C23:0, C24:0 and C25:0). These findings suggested alterations in membrane composition and hinted at a possible molecular basis for the mechanotransduction processes induced by shear stress in T. chinensis var. mairei cells.

Perfluorodecalins and Hexenol as Inducers of Secondary Metabolism in Taxus media and Vitis vinifera Cell Cultures

“…Total taxane production was highest at 24 days of culture, with a lower peak at day 12, in all the conditions studied ( Figure 3A ). The drop in total taxane yield at day 18 matches previous observations ( Bonfill et al, 2003 ; Palazón et al, 2003 ; Onrubia et al, 2010 , 2013b ), indicating that TX and related taxanes are not end-products, but can be metabolized to other compounds with similar structures ( Seki et al, 1995 ; Moon et al, 1998 ; Han et al, 2013 ). Conversely, the highest t -R levels were achieved earlier, at day 7 after elicitation ( Figure 4 ).…”

Metabolome analysis reveals ethanolamine as potential marker for improving lipid accumulation of model photosynthetic organisms

“…Metabolites extraction was conducted according to the method described by Ding et al 25 and Han et al 26 with a slight modification. A total of 20 mg of lyophilized cells were sprayed into 1 mL of methanol/water solution (1 : 1, v/v,-40…”

“…25,26 Automatic peak detection and deconvolution were performed using a peak width of 2.0 s. The signal-to-noise (S/N) values were based on the masses chosen by the software for quantification, and peaks with S/N values lower than 10 were rejected. Automatic assignments of unique fragment ions for each fatty acid or metabolite were taken as default, and manually corrected when necessary.…”

Paclitaxel-Containing Extract Exerts Anti-Cancer Activity through Oral Administration in A549-Xenografted BALB/C Nude Mice: Synergistic Effect between Paclitaxel and Flavonoids or Lignoids

“…Taxus yunnanensis Cheng et L.K. Fu is a traditional Chinese medicine, which is widely distributed in the southern regions of China [ 1 ] and is traditionally used for treating diabetes, cancer, etc [ 2 ]. It is recorded as “Hongdoushan” [ 3 ] in folk and local pharmacopeia in some regions of China, and it is abbreviated as HDS in our research.…”