Analysis of Phosphorus-containing Amino Acid-type Herbicides by Capillary Electrophoresis/Mass Spectrometry Using a Chemically Modified Capillary Having Amino Groups

Abstract

We describe a simple and practical method for the analysis of phosphorus-containing amino acid-type herbicides and their decomposition products without derivatization by capillary electrophoresis/mass spectrometry using a chemically modified capillary having amino groups. The compounds were glyphosate (GLYP), glufosinate (GLUF), bialaphos (BIAL), aminomethylphosphonic acid (AMPA) and 3-methylphosphinicopropionic acid (MPPA). AMPA and MPPA are the decomposition products of GLYP and GLUF, respectively. The optimum running conditions were found to be 100 mM formic acid adjusted to pH 3.4 with 100 mM ammonia with an applied voltage of −30 kV using an amino capillary (FunCap-CE/Type A) and mass spectrometry. The five compounds were separately determined within 10 min. Relative standard deviations of the migration times of analytes were less than 0.52%. Total analysis time of the proposed method was 1/6 to 1/3 of that of gas chromatography/mass spectrometry methods. The method was applicable for the analysis of these compounds in soil and tea beverage samples. The decomposition rates of GLYP, GLUF and BIAL in soil are discussed.

The role of derivatization techniques in the analysis of glyphosate and aminomethyl-phosphonic acid by chromatography

“…Capillary electrophoresis with electrochemiluminescence (CE-ECL) [71] and mass selective [72] detections were published recently.…”

“…Microscale solid phase extraction on alumina-coated iron oxide nanoparticles [71] was applied for GLYP and AMPA detection with a total analysis time less than 1 h (including sample pretreatment, SPE, and CE-ECL analysis). LODs for GLYP and AMPA, in water were 0.3 μg/L and 30 μg/L; in guava fruit extracts GLYP was detected only (LOD: 10 μg/1000 g).…”

“…In this review those relevant papers were compiled -without completeness -in which for GLYP and AMPA analysis -GC , LC , CEC [71,72], ion chromatography (IC) [73][74][75], spectrophotometry [76,77] and neutron magnetic resonance (NMR) [78][79][80] protocols were used. Further classifications were listed according to the matrix they had to be extracted from and/or to the detailed process they were subjected to, prior to their identification and quantitation analysis.…”

On the Relation between the Interfacial Charge of a Discharging Nozzle and Electrification of a Liquid Microjet

“…5 potentials of liquid microjets of aqueous NaI solutions using a chemically-modified fused silica capillary (GL Science FunCap-CE/Type A). 6,7 This capillary has an amino group chemically bonded to a silanol (SiOH) group on a fused silica surface, so that · surf þ · ad is always positive at pH ¼7 regardless of the concentration of NaI. After confirmation of the relation between μ and · surf þ · ad , we present a model that explains the reversal of a surface potential of a liquid microjet with the concentration of Na…”

Analysis of Phosphorus-containing Amino Acid-type Herbicides by Sheathless Capillary Electrophoresis/Electrospray Ionization-Mass Spectrometry Using a High Sensitivity Porous Sprayer

“…4 Subsequently, we developed a CE/electrospray ionization-mass spectrometry (CE/ESI-MS) method using a chemically modified capillary under acidic conditions for a rapid and reproducible analysis of these herbicides. 5 In this report, we describe a sheathless CE/ESI-MS method using a high sensitivity porous sprayer (HSPS) for the forensic analysis of phosphorus-containing amino acid-type herbicides and we compare the results with those obtained with CE/ESI-MS using a sheath liquid.…”