Analysis of Phylogenetic Affinities in the Triticinae by Protein Electrophoresis

Johnson, B. Lennart; Hall, Ove

doi:10.2307/2440267

Cited by 35 publications

(11 citation statements)

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“…(vi) Identification of parental contribution in amphiploids Zymograms of the amphiploids examined were a summation of the two parental species, a result similar to those reported by Johnson & Hall (1965) for seed proteins. Sing & Brewer (1969) were sceptical that parental contributions to polyploid species could be inferred from comparative isoenzyme studies.…”

Section: (Iii) Inter-and Intra-species Variationsupporting

confidence: 70%

Isoenzymes and polyploidy I. Qualitative and quantitative isoenzyme studies in the Triticinae

Mitra

Bhatia

1971

Genet. Res.

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Isoenzymes of alcohol (ADH), malate (MDH), glutamate (GDH) and isocitrate (IDH) dehydrogenases, and a fast migrating esterase (EST-1) were separated by disk electrophoresis from dry seed extracts of diploid, tetraploid, hexaploid and octaploid species or amphiploids belonging to the subtribe Triticinae. Only ADH and EST-1 isoenzymes showed inter-species variation; the other dehydrogenases, which show stringent substrate specificities ('critical' enzymes), revealed the same pattern in all diploid and polyploid species. The qualitative zymogram studies showed that (1) the number of variant enzyme bands increased with the level of ploidy, (2) the amphiploid isoenzyme pattern was additive of the parental species, (3) enhancement in the number of bands was due to the presence of not only parental bands, but also hybrid bands formed by association between heteromonomers. Quantitative data were obtained by densitometry of the enzyme bands as well as spectrophotometric measurements of enzyme activity in crude extracts. Increase in the level of enzyme activity was observed with ploidy level. In spite of the evidence that all duplicate/triplicate genes are expressed, increased enzyme activity observed in the polyploid species was not proportional to the level of ploidy or expected gene dosage. On the basis of ADH and EST-1 zymograms obtained in 2 x and 4 x wheat, probable zymograms for these enzymes in the B-genome donor to 4 x wheat were extrapolated. Neither Ae. speltoides nor Ae. bicornis showed the extrapolated ADH pattern. Amphiploids involving Ae. speltoides and Triticum monococcum or T. aegilopoides fully reproduced the EST-1 zymogram of 4x wheat, but not the ADH. Ae. bicornis x T. aegilopoides amphiploid showed an ADH zymogram similar to that of 4 x wheat, but the EST-1 bands were different.

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Section: (Iii) Inter-and Intra-species Variationsupporting

confidence: 70%

Isoenzymes and polyploidy I. Qualitative and quantitative isoenzyme studies in the Triticinae

Mitra

Bhatia

1971

Genet. Res.

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“…Serological analysis of pollen proteins can successfully measure the degree of genetic relationship between Citrus genotypes (SAMAAN, 1976;ABD-EL-REHIM et al ., 1978 ;SAMAAN & FADEL, 1979) . In other plants serotaxonomic studies estimated genetic relationship between species and varieties (GELL et al ., 1956 ;KLEESE & FREY, 1964;JOHNSON & HALL, 1965 ;VAUGHAN et al ., 1965;SMITH & FREY, 1970 ;HSIEH & FREY, 1972 ;EL-LAKANY et al ., 1977) .…”

Section: Resultsmentioning

confidence: 99%

Studies on the origin of Clementine tangerine (Citrus reticulata Blanco)

Samaan

1982

Euphytica

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A serological comparison of pollen proteins from `Clementine tangerine' versus those of 5 other morphologically similar Citrus cultivars was made to measure the degree of genetic relationships with 'Clementine' . Among the tested Citrus cultivars the most related to `Clementine' were 'Baladi mandarin' and 'Baladi blood orange', whereas `Sour orange' and `Satsuma mandarin' were least related . These findings support previous observations based on morphological characteristics, that the possible seed parent to 'Clementine' was 'Baladi mandarin' . The serological evidence also indicates that 'Baladi blood orange' could be the pollen parent . INTRODUCTIOǸ

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“…Electrophoretic banding homology is considered a powerful approach in estimating genetic relationships between two or more species (JOHNSON, 1968;JOHNSON et al, 1967;JOHNSON • HALL, 1965;JOHNSON 8¢ THEIN, 1970). The interrelationships, compatibility, success of crosses and different characteristics of Phaseolus species have been reported by many investigators (AL-YASIRI & COYNE, 1966;BUISHAND, 1956;COVNE, 1964;DANA, 1966;DE & KRISHNAN, 1966;FREYTAG, 1965;HONMA, 1956;PURSEGLOVE, 1968 ;SMARTT, 1970 ;STRAND, 1943 ;YARNELL, 1965).…”

Section: Discussionmentioning

confidence: 99%

An electrophoretic survey of seedling isozymes in several Phaseolus species

Bassiri

Adams

1978

Euphytica

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INDEX WORDSEsterase, acid phosphatase, peroxidase, banding pattern, tissue specificity, species relationships. SUMMARYStarch-gel electrophoresis was used to study esterase, acid phosphatase, and peroxidase isozyme patterns in the primary leaves, stems, and roots of 13 species within the genus Phaseolus (P. acutifolius, P. adenanthus, P. angularis, P. atropurpureus, P. aureus, P. bracteatus, P. calcaratus, P. lathyroides, P. mungo, P. ricciardianus, domestic P. vulgaris, and wild P. coccineus and P. vulgaris). Most species showed unique banding patterns in each isozyme system. However, a close similarity in banding patterns was observed for the domestic P. vulgaris, wild P. vulgaris, and wild P. coccinkus indicating close genetic relationships, possibly through species introgression or common descent.Great differences in isozyme patterns were found for different tissues from the same species in all systems indicating the high tissue specificity of isozymes. Upon compilation of bands from all the species, a very large number of isozymes were obtained in each system or tissue, making the electrophoretic isozyme technique of high potential in studies of isozyme variants in this genus.

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Analysis of Phylogenetic Affinities in the Triticinae by Protein Electrophoresis

Cited by 35 publications

References 0 publications

Isoenzymes and polyploidy I. Qualitative and quantitative isoenzyme studies in the Triticinae

Isoenzymes and polyploidy I. Qualitative and quantitative isoenzyme studies in the Triticinae

Studies on the origin of Clementine tangerine (Citrus reticulata Blanco)

An electrophoretic survey of seedling isozymes in several Phaseolus species

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