Analysis of plant LTR-retrotransposons at the fine-scale family level reveals individual molecular patterns

Abstract

BackgroundSugarcane is an important crop worldwide for sugar production and increasingly, as a renewable energy source. Modern cultivars have polyploid, large complex genomes, with highly unequal contributions from ancestral genomes. Long Terminal Repeat retrotransposons (LTR-RTs) are the single largest components of most plant genomes and can substantially impact the genome in many ways. It is therefore crucial to understand their contribution to the genome and transcriptome, however a detailed study of LTR-RTs in sugarcane has not been previously carried out.ResultsSixty complete LTR-RT elements were classified into 35 families within four Copia and three Gypsy lineages. Structurally, within lineages elements were similar, between lineages there were large size differences. FISH analysis resulted in the expected pattern of Gypsy/heterochromatin, Copia/euchromatin, but in two lineages there was localized clustering on some chromosomes. Analysis of related ESTs and RT-PCR showed transcriptional variation between tissues and families. Four distinct patterns were observed in sRNA mapping, the most unusual of which was that of Ale1, with very large numbers of 24nt sRNAs in the coding region. The results presented support the conclusion that distinct small RNA-regulated pathways in sugarcane target the lineages of LTR-RT elements.ConclusionsIndividual LTR-RT sugarcane families have distinct structures, and transcriptional and regulatory signatures. Our results indicate that in sugarcane individual LTR-RT families have distinct behaviors and can potentially impact the genome in diverse ways. For instance, these transposable elements may affect nearby genes by generating a diverse set of small RNA's that trigger gene silencing mechanisms. There is also some evidence that ancestral genomes contribute significantly different element numbers from particular LTR-RT lineages to the modern sugarcane cultivar genome.

Encapsidation of Host RNAs by Cucumber Necrosis Virus Coat Protein during both Agroinfiltration and Infection

“…6B and C). The retrotransposons included both major superfamilies of long terminal repeat (LTR) class I transposons, Gypsy and Copia, which are abundant in plant genomes, as well as LINE-1 transposons, which are non-LTR class I transposons also known to be present in plants (51)(52)(53)(54)(55) (Fig. 7).…”

Building the sugarcane genome for biotechnology and identifying evolutionary trends

“…TEs were screened using RepeatMasker [54] against repeats from Viridiplantae [55] and sugarcane LTR retrotransposons [10], with a cut-off score of 250. Both complete and incomplete elements were identified.…”

Virus-Like Attachment Sites and Plastic CpG Islands: Landmarks of Diversity in Plant Del Retrotransposons

“…Within these superfamilies, evolutionary lineages have been identified which have distinct patterns in terms of structure, expression, regulation and chromosomal distribution [10], [17]. Del, a Ty3/Gypsy lineage, has the largest described LTRs, and also the largest LTR length variation, from 1.1 to 4.4 kb [18].…”