Analysis of Plant Metabolites Damaged in Chemical Accidents Using NMR Spectroscopy

Abstract

Chemical accidents can cause a variety of damage, including damage to human health and ecosystems. In Korea, there are a limited number of crops that can be used to calculate damage done to plants after chemical accidents, and there are insufficient data on testing of damaged plants and methods for evaluation thereof because damage calculation methods and procedures have not been specifically established. Therefore, in order to estimate economic losses due to chemical accidents, it is necessary to establish specific calculation methods and procedures. At this point, improvements in Nuclear Magnetic Resonance (NMR) spectroscopy are needed to improve the accuracy and precision of assessment of plant damage caused by chemical accidents. The purpose of this study is to standardize sample pretreatment methods for NMR application and to develop a methodology for estimation of plant damage via testing and evaluation methods for solid samples such as plants and powders at chemical accident sites.

Structural Studies of Expressed tIK, Anti-Inflammatory Peptide

“…A solution state 2D 1 H- 15 N heteronuclear single quantum coherence (HSQC) NMR experiment was performed by uniformly expressing the 15 N-labeled tIK peptide series. The 1 H- 15 N HSQC experiments showed cross-peaks correlated with a proton directly bonded to 15 N [24]. Therefore, each cross-peak represented one amino acid.…”