Analysis of point mutations of the BRCA1 gene by hybridization with hydrogel microarrays

Федорова, О Е; Siņicka, Olga; Tihomirova, Laima; Visnevskaya, Ya. V.; Заседателев, А. С.; Наседкина, Т. В.

doi:10.1134/s0026893306010055

Cited by 12 publications

(2 citation statements)

References 20 publications

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“…However, in the S30 extract they all have substantial activity. As the relative translation efficiencies in the nuclease untreated S30 extract reflect much better the situation observed in transfected cells, we conclude that RRL is not an adequate system to study the translation initiation mechanisms for mRNAs with complex 5′ UTRs, a conclusion that had been already made in our recent reports (16,17). …”

Section: Resultsmentioning

confidence: 61%

Differential contribution of the m7G-cap to the 5′ end-dependent translation initiation of mammalian mRNAs

Andreev

Dmitriev

Terenin

et al. 2009

Nucleic Acids Research

100

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Many mammalian mRNAs possess long 5′ UTRs with numerous stem-loop structures. For some of them, the presence of Internal Ribosome Entry Sites (IRESes) was suggested to explain their significant activity, especially when cap-dependent translation is compromised. To test this hypothesis, we have compared the translation initiation efficiencies of some cellular 5′ UTRs reported to have IRES-activity with those lacking IRES-elements in RNA-transfected cells and cell-free systems. Unlike viral IRESes, the tested 5′ UTRs with so-called ‘cellular IRESes’ demonstrate only background activities when placed in the intercistronic position of dicistronic RNAs. In contrast, they are very active in the monocistronic context and the cap is indispensable for their activities. Surprisingly, in cultured cells or cytoplasmic extracts both the level of stimulation with the cap and the overall translation activity do not correlate with the cumulative energy of the secondary structure of the tested 5′ UTRs. The cap positive effect is still observed under profound inhibition of translation with eIF4E-BP1 but its magnitude varies for individual 5′ UTRs irrespective of the cumulative energy of their secondary structures. Thus, it is not mandatory to invoke the IRES hypothesis, at least for some mRNAs, to explain their preferential translation when eIF4E is partially inactivated.

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Section: Resultsmentioning

confidence: 61%

Differential contribution of the m7G-cap to the 5′ end-dependent translation initiation of mammalian mRNAs

Andreev

Dmitriev

Terenin

et al. 2009

Nucleic Acids Research

100

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“…According to the data of a number of researchers, the BRCA1 5382insC mutation is the most frequent in Russia. For instance, the studies carried out in Mos cow and St. Petersburg showed that the percentage of the BRCA1 5382insC carriage among the BRCA patients constituted 3 to 4% [18][19][20].…”

Section: Frequencies Of the Mutations Examinedmentioning

confidence: 98%

Prevalence of mutations BRCA1 5382insC, and CHEK2 1100delC in the population of Siberian region

et al. 2012

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Frequencies of the 5382insC mutation in the BRCA1 gene and the 1100delC mutation in the CHEK2 gene were compared in the group of breast cancer patients and the large scale sample, consisting of 7920 DNA specimens from healthy residents of the city of Novosibirsk. Higher frequencies of these mutations in the patient group compared to the control sample (1.95% versus 0.25% for BRCA1 5382insC, and 1.78% versus 0.40% for CHEK2 1100delC) were observed, pointing to their association with susceptibility to breast cancer (OR = 7.86; 95% CI 3.51-17.30 and OR =4.46: 95% CI 2.

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A researcher’s guide to the galaxy of IRESs

Terenin

Smirnova

Andreev

et al. 2016

Cell. Mol. Life Sci.

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The idea of internal initiation is frequently exploited to explain the peculiar translation properties or unusual features of some eukaryotic mRNAs. In this review, we summarize the methods and arguments most commonly used to address cases of translation governed by internal ribosome entry sites (IRESs). Frequent mistakes are revealed. We explain why "cap-independent" does not readily mean "IRES-dependent" and why the presence of a long and highly structured 5' untranslated region (5'UTR) or translation under stress conditions cannot be regarded as an argument for appealing to internal initiation. We carefully describe the known pitfalls and limitations of the bicistronic assay and artefacts of some commercially available in vitro translation systems. We explain why plasmid DNA transfection should not be used in IRES studies and which control experiments are unavoidable if someone decides to use it anyway. Finally, we propose a workflow for the validation of IRES activity, including fast and simple experiments based on a single genetic construct with a sequence of interest.

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Analysis of point mutations of the BRCA1 gene by hybridization with hydrogel microarrays

Cited by 12 publications

References 20 publications

Differential contribution of the m7G-cap to the 5′ end-dependent translation initiation of mammalian mRNAs

Differential contribution of the m7G-cap to the 5′ end-dependent translation initiation of mammalian mRNAs

Prevalence of mutations BRCA1 5382insC, and CHEK2 1100delC in the population of Siberian region

A researcher’s guide to the galaxy of IRESs

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