Analysis of poly(ethylene glycol)‐diacrylate macromer polymerization within a multicomponent semi‐interpenetrating polymer network system

Abstract

Semi-interpenetrating polymer networks (semi-IPNs) containing poly(ethylene glycol)-diacrylate (PEGdA) and modified gelatin were prepared with 2,2-dimethoxy-2-phenylacetophenone (DMPA) as a photoinitiator. The effect of (i) initiator and PEGdA concentration, and (ii) weight ratio and type of modified gelatin on the conversion of PEGdA functional end groups was monitored in situ using attenuated total reflectance-Fourier transform infrared (ATR-FTIR). Reaction induction time was dependent on DMPA concentration and increased with decreasing DMPA concentration. Relative reaction rate was strongly dependent on both DMPA and PEGdA concentrations. Gelatin weight ratio and modification did not significantly affect reaction induction time, relative reaction rate, or reaction end time. Swelling/degradation kinetics at various aqueous conditions sought to establish relationships between diacrylate conversion and the resulting semi-IPN physical properties. Semi-IPN swelling weight ratio was strongly dependent on solvent conditions and semi-IPN exposure to gamma-irradiation. Gelatin backbone modification and UV exposure time exhibited no effect on semi-IPN swelling weight ratio. In conclusion, ATR-FTIR presents a viable means of monitoring the conversion of PEGdA functional end groups within a complex mixture. UV exposure >10 s did not significantly affect the weight swelling ratio, and supports our ATR-FTIR results that network formation reached completion before 3 min of UV exposure.

Interpenetrating polymer networks containing gelatin modified with PEGylated RGD and soluble KGF: Synthesis, characterization, and application in in vivo critical dermal wound

“…17 PEGdA (600 Da) was then added to this mixture in a 4:6 gelatin:PEGdA weight ratio to form a 10 wt % solution. 24 Next, 20 mL of an initiator solution containing 2,2-dimethoxy-2-phenyl-acetophenone (DMPA) (0.02 g DMPA in 0.5 g PEGdA) was added to the mixture and vortexed. This solution was made immediately prior to the surgery.…”

“…22,23 The following procedure for IPN synthesis is adapted from previously established procedures. 21,24 PEGylated-RGD-modified or unmodified gelatin was dissolved in deionized water at a concentration of 0.1 g/mL at 608C to make RGDmodified gelatin IPNs (RGD-IPNþKGF) and unmodified gelatin IPNs (unmod-IPN), respectively. KGF-1 (QED Bioscience, 90% purity) was added to the RGD-modified gelatin solution at a concentration of 3.3 ng/mL which has been shown to stimulate keratinocyte proliferation.…”

Spatially Resolved Measurements of Crosslinking in UV-Curable Coatings Using Single-Sided NMR

“…The IR spectra are normalized to the peak at 1726 cm −1 , corresponding to the acrylic carbonyl group; these carbonyl groups are largely unaffected during curing and their IR intensities should remain fixed. In contrast, the terminal CH=CH 2 bonds on the PEGDA monomers produce a peak at 1410 cm −1 [17][18][19][20]. A decrease in the intensity of the 1410 cm −1 peak when the sample is exposed to UV light indicates curing.…”

Extended interaction of β1 integrin subunit-deficient cells (GD25) with surfaces modified with fibronectin-derived peptides: Culture optimization, adhesion and cytokine panel studies

“…The following procedure for sIPN synthesis is adapted from previously established procedures [39,40]. Methoxy-PEGylated-modified, PEGylated-GGG-modified, PEGylated-RGDmodified, PEGylated-PHSRN-modified or unmodified gelatin was dissolved in deionized water at a concentration of 0.1 g ml −1 at 60°C to synthesize methoxy-PEG-modified gelatin sIPNs (mPEG-sIPN), PEGylated-GGG-modified gelatin sIPNs (GGG-sIPN), RGD-sIPN, PHSRN-sIPN, and unmodified gelatin sIPNs (Unmod-sIPN or mold-cast Unmod-sIPN), respectively.…”