Analysis of protein content and level of 7S and 11S globulins, their ratio in the soybean seed of F6 - F8 hybrid lines

Abstract

Aims. The goal of research was to study a character of changes and relationship between level of 7S and 11S globulins, their ratio and protein content in the soybean seed of F6-F8 hybrid lines and their parental forms of different genetic origin in the connection of protein quality breeding. Methods. 7S and 11S globulins were isolated by method, which was developed in the Laboratory of Plant Biochemistry (Patent # 42181). Results. The features of 7S and 11S globulins content and their ratio in the soybean seed of F6 -F8 hybrid lines and their parental forms of different genetic origin were identified. It was established, that varieties and hybrid lines are characterized by considerable distinctions of 7S and 11S globulins contents and their ratio in the soybean protein. A connection between 7S and 11S globulins levels and protein content in the seed of soybean F6- F8 hybrid lines wasn’t established. Conclusions. It was elucidated that the growth conditions influence on the protein content, 11S and 7S globulins level, their ratio and relationship between these parameters in the soybean seeds of F6-F8 hybrid lines and their parental forms of different genetic origin, so that have to be introduced in the selection program of soybean genotypes of food direction soybean breeding as these parameters connected with the food nutritional quality.Key words: Glicine max L., protein, 11S globulin, 7S globulin.