Analysis of protein expression changes in the blood plasma of cows during the last month before parturition and 2 months after calving

Kurpińska, Anna; Jarosz, Agnieszka; Ożgo, M.; Skrzypczak, W. F.

doi:10.3906/biy-1501-30

Cited by 9 publications

(8 citation statements)

References 43 publications

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“…Glucose regulates the concentrations of other blood metabolites [6], and low blood glucose in the PPP causes repeat breeding and reduces the conception rate (CR) [7,8]. Many protein-related parameters are highly affected around parturition and may significantly impose to further reproductive performance [9]. Animals require minerals such as calcium (Ca), magnesium (Mg), and phosphorus (P) during late pregnancy and the postpartum period for growth, reproduction, and lactation, which often affect specific requirements and serve as catalytic components of enzymes or regulate several mechanism involved in pregnancy and lactation [10,11].…”

Section: Introductionmentioning

confidence: 99%

Determination of Blood Glucose, Total Protein, Certain Minerals, and Triiodothyronine during Late Pregnancy and Postpartum Periods in Crossbred Dairy Cows

Mohammed

Ahmad

Frah

et al. 2021

Veterinary Medicine International

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The late pregnancy (3rd trimester) and the postpartum period (PPP) (calving date or day zero to day 45) are very critical periods for the fertility and production in dairy cows. This study was designed to investigate blood glucose, total protein (TP), calcium (Ca), phosphorus (P), magnesium (Mg), iron (Fe), and triiodothyronine (T3) during late pregnancy and the PPP. Twenty-seven apparently healthy multiparous crossbred dairy cows (Friesian × Kenana) were included in this study. The cows were randomly allocated into three groups: group A (n = 10), cows with late pregnancy, group B (n = 7), cows in the PPP, and group C (n = 10), nonpregnant cows as control. One-way ANOVA was used to analyze the data. The results of this study showed that blood glucose was higher in late pregnancy and the PPP than in nonpregnant cows. The TP was significantly lower in late pregnant cows than during the PPP and in nonpregnant cows. Ca, P, and Mg were not significantly different between periods. Serum Fe and T3 were significantly lower during the PPP than that in late pregnant and nonpregnant cows. The results can provide indications of the nutritional status of dairy cows and a diagnostic tool to avoid the metabolic disorders that may occur during late pregnancy and the PPP.

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Section: Introductionmentioning

confidence: 99%

Determination of Blood Glucose, Total Protein, Certain Minerals, and Triiodothyronine during Late Pregnancy and Postpartum Periods in Crossbred Dairy Cows

Mohammed

Ahmad

Frah

et al. 2021

Veterinary Medicine International

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“…Many protein-related parameters are highly affected around parturition and may significantly impose to further reproductive performance [Kurpińska et al 2015, Kurpińska et al 2016. Gonzales and Rocha [1998] reported that higher levels of urea and lower levels of protein are noted in cows with prolonged inter-pregnant period and Jorritsma et al [2003] underlined that urea may impact the oocyte development, fertilization and embryo development.…”

Section: Introductionmentioning

confidence: 99%

Parameters of protein and iron metabolism in dairy cows during periparturient period

Kurpińska¹,

Jarosz²,

Skrzypczak³

2020

Acta Sci. Pol. Zootech.

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Knowledge of the changes in the concentrations of biochemical parameters gives the possibility to conclude about the health status of the cows, the quality of nutrition, or reproductive performance and milk productivity. The studies aimed to assess the health status of the primiparous dairy cows at the time of prospectus new fertilization and to screen the challenges of the preceding periparturient period through the analysis of protein metabolism and iron parameters in context of their potential role as markers of the reproductive status. Despite significant variations, the studies showed that homeostatic mechanisms and adaptational processes in cows organism are rather efficient during this period. Nevertheless, it should be underlined that at the time of prospectus new pregnancy higher concentration of urea, lower concentration of creatinine and iron parameters was noted, emphasizing the dysregulation of the organism condition and pointing the possible reason of/association with prospectus difficulties in further reproductive performance. If analyzed for the particular herd, these parameters may be useful in considerations about the selection of the time for new fertilization.

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“…Kurpińska et al. () examined bovine plasma using 2DE and MS identification. Authors collected samples at similar time frames as in present study but identified different proteins—23 spots represented 13 identified proteins of 283 detected spots.…”

Section: Discussionmentioning

confidence: 99%

“…There are few reports about the analysis of periparturient plasma protein profile in cows but due to different sample protocols as well as different criteria for the selection of spots for identification, full profile was not established yet (Kurpińska, Jarosz, Ożgo, & Skrzypczak, ; Ma, Bu, Yang, Yan, & Wang, ; Yang et al., ).…”

Section: Introductionmentioning

confidence: 99%

Preliminary data on possible protein markers of parturition in cows

Wawrzykowski

Franczyk

Hoedemaker

et al. 2017

Reprod Domestic Animals

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Parturition is one of the most important events in reproduction. Regardless of many studies, exact time for pregnancy termination and onset of parturition is impossible to determine. The aim of this study was to describe and to compare protein profile of plasma from healthy pregnant cows (n = 6) at following five time points: 2 weeks, 1 week before, at parturition, 1 week and 2 weeks after parturition to search for possible protein markers of parturition. Plasma samples were analysed by 1D and 2D electrophoresis, and selected spots were identified by mass spectrometry. Protein profile showed no uniform pattern. Seventy spots differed at least for one sampling point from the time point 2 weeks before parturition which served as reference. Thirty spots expressed higher intensity of staining 1 week as 2 weeks before parturition while 13 showed opposite relationship. Twenty two spots expressed higher intensity of staining at parturition as 2 weeks before delivery while 15 showed opposite relationship. Eighteen spots expressed higher intensity of staining 2 weeks before parturition as 1 week post-partum while 2 showed opposite relationship. Fifteen spots expressed higher intensity of staining 2 weeks before parturition as 2 weeks after delivery while 14 showed opposite relationship. Thirty-five proteins, belonging to different functional groups, were identified. Of them, 15 spots differed significantly between parturition and 2 weeks before delivery. Among them were metalloproteinase inhibitor and LDH which seem to be the most promising molecules considered as parturition markers due to their functions.

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Analysis of protein expression changes in the blood plasma of cows during the last month before parturition and 2 months after calving

Cited by 9 publications

References 43 publications

Determination of Blood Glucose, Total Protein, Certain Minerals, and Triiodothyronine during Late Pregnancy and Postpartum Periods in Crossbred Dairy Cows

Determination of Blood Glucose, Total Protein, Certain Minerals, and Triiodothyronine during Late Pregnancy and Postpartum Periods in Crossbred Dairy Cows

Parameters of protein and iron metabolism in dairy cows during periparturient period

Preliminary data on possible protein markers of parturition in cows

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