Analysis of Proteins andDNAsUsing Inductively Coupled Plasma Mass Spectrometry and Elemental Tagging

Abstract

Inductively coupled plasma mass spectrometry (ICP‐MS) is one of the most powerful techniques allowing multielement and/or isotope quantification over broad linear dynamic range with very low detection limit. Combining with elemental tagging strategies, ICP‐MS has facilitated multiplexed analysis of both proteins and nucleic acids, which contributes to bioanalytical science. This article describes different types of bioanalytical applications for detecting these biomolecules. Common conjugation approaches and elemental tags used in these assays are reviewed. Analysis of more than 30 proteins and DNAs of 15 different sequences has been achieved using elemental tags combined with ICP‐MS. ICP‐MS‐based biomolecules assay will undoubtedly be developed in the future for biological research.

Quantitative mapping of specific proteins in biological tissues by laser ablation–ICP-MS using exogenous labels: aspects to be considered

“…There is no doubt that the combination of IHC with spectroscopic and MS techniques offers great possibilities for quantitative imaging of specific proteins in biological tissues, with detailed aid in experimental design provided in previous reviews [3]. Finally, it is worth to mention that research in this field can be extended to other cutting edge studies, such as the elucidation of mechanisms related with antibody-drug conjugates for oncology therapeutics [67,68], as well as for nucleic acids (DNA and RNA) assays [69]. [24] with permission of Springer.…”

Integral Multielement Signals by DNA-Programmed UCNP–AuNP Nanosatellite Assemblies for Ultrasensitive ICP–MS Detection of Exosomal Proteins and Cancer Identification

“…Second, the rare element label in the form of nanoparticles can amplify the ICP–MS signal and further improve the detection sensitivity conveniently. For example, NaHoF 4 nanoparticles with a diameter of 12.9 nm contain up to 15,000 Ho atoms, and Au nanoparticles with a diameter of 10 nm contain about 30,000 Au atoms . Third, ICP–MS can simultaneously analyze multiple signals at high throughput and fast speed, thereby simplifying the operation steps of multiplexed information acquirement.…”

“…For example, NaHoF 4 nanoparticles with a diameter of 12.9 nm contain up to 15,000 Ho atoms, and Au nanoparticles with a diameter of 10 nm contain about 30,000 Au atoms. 21 Third, ICP−MS can simultaneously analyze multiple signals at high throughput and fast speed, thereby simplifying the operation steps of multiplexed information acquirement. Finally, considering that the expression level of single marker on exosomes is variable between patients due to the heterogeneity of cancer phenotypes, 22,23 chemical "nose/tongue"-based sensor array methods provide a powerful data analysis strategy for the identification of complex biological analytes.…”