Analysis of Proteus mirabilis proteins by using SDS-page technique in rheumatoid arthritis patients

Abstract

This study was aimed to detecting difference in proteins separation by SDS-Page for Proteus mirabilis isolated from urine of non-Rheumatoid arthritis patients have urinary tract infection , urine from Rheumatoid arthritis patients have urinary tract infection. Two hundred (200) urine sample collected from non RA patient30 urine sample from RA patient . These clinical samples were collected and cultured on blood agar and macConky agar. 32 isolates were identified as p. mirabilis by using biochemical test and VITEK 2 system used to complete the identification. The current study used SDS-Page technique for proteins analysis of Proteus mirabilis for both RA patient and non RA patient to detect the differences between them and the result appeared that there are no differences in total protein, insoluble proteins and soluble protein at different pH. Values (6.0, 7.0 & 8.0) at 37 cº, also the result shown differences in the cell –free culture supernatants between Proteus mirabilis from urine of both rheumatoid arthritis patients and non-rheumatoid arthritis patient in pH.8.0 at 37 cº in 7 h. by band appeared in pH.8.0 at 37 cº in 7 h in RA .and there is no band in non RA at the same condition.