Analysis of PTEN expression in large intestine polyps and its relation to the recognized histopathological and clinical risk factors for cancer development in this location

Waniczek, Dariusz; Śnietura, Mirosław; Pigłowski, Wojciech; Rdes, Jerzy; Kopeć, Agnieszka; Młynarczyk-Liszka, Joanna; Rudzki, Marek; Hudyka, Krystyna; Arendt, Jerzy; Lange, Dariusz

doi:10.5114/wo.2012.30059

Cited by 5 publications

(4 citation statements)

References 24 publications

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“…PTEN specificity of that antibody was confirmed earlier, using Western blotting and immunohistochemistry techniques in several cell lines of known PTEN status [12]. Immunostaining was performed according to standard guidelines: four-micrometer-thick sections were cut, mounted on slides, deparaffinized, and rehydrated in xylene and in a graded series of ethanol.…”

Section: Immunohistochemistrymentioning

confidence: 86%

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PTEN expression profiles in colorectal adenocarcinoma and its precancerous lesions

Waniczek¹,

Śnietura²,

Młynarczyk-Liszka³

et al. 2013

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The aim of the study was to determine expression of the PTEN suppressor gene in colorectal adenocarcinoma and its precancerous lesions (adenomatous polyps) in correlation with common clinical and histopathological features. Forty-four patients with adenomatous polyps and 32 with primary adenocarcinoma of the colon or rectum were enrolled in the study. They underwent endoscopic removal of polyps or major surgery and postoperative adjuvant chemo-and radiotherapy depending on staging of the disease. No patient had received chemotherapy and/or radiotherapy before the surgery. PTEN expression was evaluated using immunohistochemical staining on paraffin-embedded specimens and compared to clinicopathological features of tumors. In colorectal cancers, PTEN expression was found to be significantly lower than in normal intestinal mucosa and adenomatous polyps. That was associated with complete loss of PTEN expression observed more frequently in colorectal cancer, contrary to reduction of PTEN expression occurring mostly in polyps. A correlation between polyp diameter and loss of PTEN was demonstrated as well as between tumor size and TNM advanced stage and PTEN expression. The obtained results suggest that the PTEN/PI3K/Akt pathway may play an important role in early stages of sporadic colorectal carcinogenesis and reduced PTEN expression in late oncogenesis is associated with some adverse clinical and pathological features.

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Section: Immunohistochemistrymentioning

confidence: 86%

“…Representative areas of tumor were selected and scored un-DARIUSZ WANICZEK, MIROSLAW SNIETURA, JOANNA MLYNARCZYK-LISZKA, ET AL. [12] served as a positive control. Additional tumor sections, treated as described above but without primary antibody application, revealed no staining (negative control).…”

Section: Immunohistochemistrymentioning

confidence: 99%

PTEN expression profiles in colorectal adenocarcinoma and its precancerous lesions

Waniczek¹,

Śnietura²,

Młynarczyk-Liszka³

et al. 2013

pjp

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“…The literature supports progressive PTEN expression loss in colorectal carcinogenesis stages with the lowest expression in malignant lesions 47 ; however, differences between polyp types were not reported. 48 The colorectal tumorigenesis process is usually slow, with adenomas appearing usually 10 to 15 years prior to colorectal adenocarcinomas. 49 How PTEN behaves at critical tumorigenesis stages is unknown.…”

Section: Discussionmentioning

confidence: 99%

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2018

Journal of Coloproctology

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Objectives: Determine immunohistochemical expression of Phosphatase and tensin homolog (PTEN), Phosphatidylinositol 3 kinase (PI3K), Cycloxygenase-2 (COX2) and one proliferation marker (Ki67) in colorectal polyps and correlate with clinical and pathological data in search of carcinogenic pathways. Methods:The reports of 297 polyps diagnosed through endoscopy were reviewed for parameters including age, gender, prior colorectal cancer, the presence of multiple polyps, and polyps' location, appearance and size. Was conducted a microscopic morphometric computerized analysis of immunohistochemical expression using, the selected antibodies and correlated with clinical and pathological variables.Results: The tissue immunohistochemical expression was higher in right colon polyps for the proliferation marker and Phosphatidylinositol 3 kinase (p ≤ 0.0001 and 0.057 respectively).Cycloxygenase-2 and Phosphatase and tensin homolog demonstrated higher tissue immunoexpression in pedunculated polyps (p = 0.009 and 0.002 respectively). Cycloxygenase-2 exhibited higher immunoexpression in larger polyps (p = 0.005). Phosphatidylinositol 3 kinase, Cycloxygenase-2, Phosphatase and tensin homolog and the proliferation marker exhibited higher immunoexpression in high-grade dysplastic polyps (p = 0.031, 0.013, 0.044 and <0.001 respectively). Phosphatase and tensin homolog labeling was higher in polyps with high-grade dysplasia and lower in some of serrated lesions (p = 0.044). Conclusions:The greater expression of the proliferation marker and Phosphatidylinositol 3 kinase in the right colon may be related to right-sided colorectal carcinogenesis. The

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“…PTEN function enables the maintenance of normal tissues architecture by affecting the intercellular connections stabilisation, cell polarity and migration [24]. The inactivation of PTEN in combination with agents in tumour micro-environment, suchas TNF-α, may increase the CRC invasiveness[25].THE PTEN EXPREssION AND THE sTAGE OF cARcINOGENEsIsThere is considerable evidence indicating a gradual reduction of PTEN expression in the sequence of transformations: normal tissue-polyp-adenocarcinoma-distant metastases[5,[26][27][28].Different results were obtained by Colakoglu et al, indicating more frequent lack of PTEN expression (referred to as "0" by immunohistochemistry, IHC) in colonic adenomatous polyps vs. colorectal cancer specimens (40% vs. 5.3%; p < 0.0001). The authors hypothesized that a decrease in PTEN function may play a particular role in the early stages of neoplastic transformation.…”

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confidence: 99%

PTEN – clinical significance in colorectal cancer

et al. 2016

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Phosphatase and tensin homolog deleted on chromosome ten (PTEN) is a human suppressor gene. Its protein product is a bispecific phosphatase playing the complex role in the cell cycle regulating processes and apoptosis by the mechanism of signal transduction into the cell via tyrosine kinase B signaling pathway (PI3K/Akt/mTOR). Reduction or loss of PTEN function is implicated in the pathogenesis of many malignancies, including colorectal cancer. A gradual decrease in the function of PTEN in the sequence of transformations: normal tissue-polyp-adenocarcinoma-disseminated cancer was indicated. The relation between the PTEN loss and the higher clinical severity of colorectal cancer was observed, i.a. higher TNM status and higher tendency to form metastases, leading in some of the studies to shortened patients survival during the observation period. The potential predictive value of the PTEN function loss for the EGFR-targeted therapy in patients with advanced colorectal cancer is the subject of controversy. The potential application of PTEN assessment in clinical practice as a prognostic and/or predictive factor requires further well-designed prospective studies on larger patient population, using the unified methodology. The aim of the study is to summarize the current knowledge on the role of PTEN gene and PTEN protein in the pathogenesis of colorectal cancer and the role of PTEN in clinical practice.

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Analysis of PTEN expression in large intestine polyps and its relation to the recognized histopathological and clinical risk factors for cancer development in this location

Cited by 5 publications

References 24 publications

PTEN expression profiles in colorectal adenocarcinoma and its precancerous lesions

PTEN expression profiles in colorectal adenocarcinoma and its precancerous lesions

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PTEN – clinical significance in colorectal cancer

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