Analysis of rabbit β-like globin gene transcripts during development

Rohrbaugh, Mark L.; Hardison, Ross C.

doi:10.1016/0022-2836(83)90058-x

Cited by 26 publications

(10 citation statements)

References 40 publications

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“…The gamma-globin genes of both the prosimian primate, galago, and species in order Glires (rabbit, mouse and rat) are expressed in embryonic erythroid cells (Rohrbaugh and Hardison 1983; Whitelaw et al 1990; Satoh et al 1999), whereas the gamma-globin genes of anthropoid primates (monkeys, apes and humans) are expressed only in fetal erythroid cells (Johnson et al 1996; Johnson et al 2000) (Fig. 4).…”

Section: Hemoglobin Gene Clusters In Jawed Vertebratesmentioning

confidence: 99%

Evolution of Hemoglobin and Its Genes

Hardison

2012

Cold Spring Harbor Perspectives in Medicine

146

107

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Insights into the evolution of hemoglobins and their genes are an abundant source of ideas about hemoglobin function and regulation of globin gene expression. This chapter presents the multiple genes and gene families encoding human globins, summarizes major events in the evolution of the hemoglobin gene clusters, and discusses how these studies provide insights into regulation of globin genes. While the genes in and around the alpha-like globin gene complex are relatively stable, the beta-like globin gene clusters are more dynamic, showing evidence of transposition to a new locus and frequent lineage-specific expansions and deletions. The cis-regulatory modules controlling levels and timing of gene expression are a mix of conserved and lineage-specific DNA, perhaps reflecting evolutionary constraint on core regulatory functions shared broadly in mammals and adaptive fine-tuning in different orders of mammals.

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Section: Hemoglobin Gene Clusters In Jawed Vertebratesmentioning

confidence: 99%

Evolution of Hemoglobin and Its Genes

Hardison

2012

Cold Spring Harbor Perspectives in Medicine

146

107

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“…The two y-globin genes are very similar because of a recent duplication followed by gene conversion (Slightom et al 1980;Shen et al 198 1). The pattern of expression of p-like globins differs substantially between rabbits and humans Rohrbaugh and Hardison 1983). Rabbits have two embryonic genes, p3 and p4, whereas humans have one, E. Humans have duplicated, fetal specific genes, 9 and G'y, whereas rabbits have none.…”

Section: Pairwise Comparisons Between Rabbit and Human P-like Globin mentioning

confidence: 99%

“…This family is composed of four genes arranged in the order 5'-p4-p3-~p2-p1-3' . Genes p4 and, p3 are expressed only in embryonic erythrocytes; gene pl is expressed in fetal and adult red cells Rohrbaugh and Hardison 1983); and vIp2 is a pseudogene incapable of producing a functional globin (Lacy and Maniatis 1980). The sequences of two alleles of pl van Ooyen et al 1979) and one allele each of @2 (Lacy and Maniatis 1980;Hardison and Margot 1984), p3 (Hardison 1981), and P4 (Hardison 1983) have been published.…”

Section: Introductionmentioning

confidence: 99%

Comparison of the beta-like globin gene families of rabbits and humans indicates that the gene cluster 5'-epsilon-gamma-delta-beta-3' predates the mammalian radiation.

1984

Molecular Biology and Evolution

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The members of the rabbit and human P-like globin gene families have been compared both by a computer-generated dot matrix graphical analysis of each entire gene and by calculating divergences in the coding regions. The rabbit-human gene pairs P~-E, p3-y, vp2-6, and p 1 -p were identified as orthologous on the basis of sequence similarities found in flanking and intervening sequences as well as by quantitative divergence calculations. The orthologous genes are in the same order on the chromosome in each species, which suggests that an ancestral family with the arrangement S-a-y-6-p-3 preceded the mammalian radiation. Descendants of ancestral E have diverged more slowly than other P-like genes and are expressed only in embryonic life. Descendants of ancestral y and p diverged at a higher rate and are expressed at wider range of developmental times. Descendants of 6 have undergone nonreciprocal recombination at a high frequency and are often pseudogenes. Paralogous comparisons among the rabbit P-like globin genes show that the p4-p3 and w/32-p1 pairs are most similar and that p4 and p3 are more closely related to pl than to wp2. This fits with a branching pattern where the primordial p split into a ncestral a/y and 6/p genes, which later split into a and y or 6 and p, respectively. Rabbit genes p4 and p 1 acquired similar 3' untranslated regions after the E/Y split but prior to the mammalian radiation, presumably via a gene conversion event. The 5' end of j32 apparently converted with pl after the radiation, and afterward it became a pseudogene.

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“…Gene 133 and 134 are expressed in embryonic life, gene 13 is expressed in fetal and adult life (22,50), and gene 4432 is an unexpressed pseudogene (33). The coordinate change in the amounts of polypeptides, mature mRNAs, and pre-mRNAs from each active gene suggests that the switch in expression from embryonic to fetal-adult 1-like globin genes is accomplished primarily by differential transcription of the genes (50). Transcriptional regulation of rabbit 13-globin gene expression is demonstrated more directly in this paper.…”

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confidence: 99%